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Sheep placenta antioxidant polypeptide as well as enzymatic hydrolysis preparation method and application thereof

An anti-oxidant peptide and sheep placenta technology, applied in the preparation method of peptide, chemical equipment and method, cosmetic preparations, etc., to achieve the effect of increasing added value, good activity and high purity

Active Publication Date: 2017-02-22
江苏艾玛仕生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] To sum up, there is no existing use of sheep placenta scraps as raw materials to further utilize them, and to obtain polypeptide products with functional activity, especially antioxidant activity, through optimized process operations; therefore, how to realize full utilization of resources and improve product quality Adding value and obtaining more natural and efficient cosmetics has become a major technical problem in this field that needs to be solved urgently

Method used

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  • Sheep placenta antioxidant polypeptide as well as enzymatic hydrolysis preparation method and application thereof
  • Sheep placenta antioxidant polypeptide as well as enzymatic hydrolysis preparation method and application thereof
  • Sheep placenta antioxidant polypeptide as well as enzymatic hydrolysis preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] The sheep placenta was made into a 10% homogenate with a tissue homogenizer, and after repeated freezing and thawing three times, it was centrifuged at low temperature to collect the residue, which was freeze-dried, crushed with a pulverizer, and passed through a 60-mesh sieve to obtain dry powder of sheep placenta scraps.

[0079] Weigh 3.3g dry powder of sheep placenta scraps, add 1000mL deionized water, rapidly raise the temperature to 90°C, keep the temperature for 15min, then cool down to 55°C, adjust the pH to 6.5 with 0.2mol / L HCl, add 1.27g papain for enzymatic hydrolysis for 120min After hydrolysis, heat up to 90°C, keep for 20min for enzyme inactivation treatment, cool to room temperature, adjust pH to neutral with 0.2mol / L NaOH, centrifuge at 4°C and 8000r / min for 20min at low temperature, collect the supernatant, Freeze-dried and set aside.

[0080] Prepare a 10mg / mL polypeptide solution, use DA201-C macroporous adsorption resin for sugar removal and desalin...

Embodiment 2

[0082] The antioxidant polypeptide prepared in Example 1 was used to prepare an anti-aging lotion added with sheep placental antioxidant polypeptide, and the formula is shown in Table 2. The preparation process of skin care lotion is as follows:

[0083] (1) Record the weight of the empty cup, weigh the components of phase A in sequence according to the formula table, sterilize in boiling water for 15 minutes, and then replenish the evaporated water;

[0084] (2) Weigh the components of phase B in sequence according to the formula table, and fully dissolve them in boiling water for 15 minutes;

[0085] (3) After the two phases are fully dissolved, cool down to 80°C, slowly pour phase B into phase A, homogenize at 4000r / min for 5 minutes, cool to 45°C, add flavor, preservative and sheep placenta antioxidant polypeptide;

[0086] (4) Vacuumize to remove air bubbles, stir and cool, then discharge and fill.

[0087] The MC 750 skin tester of German CK Company was used to evaluat...

Embodiment 3

[0108] The antioxidant polypeptide prepared in Example 1 was used to prepare an anti-aging skin care night cream added with sheep placenta antioxidant polypeptide, and the formula is shown in Table 3.

[0109] The preparation process of skin care night cream is as follows:

[0110] (1) Record the weight of the empty cup, weigh the components of phase A in sequence according to the formula table, sterilize in boiling water for 15 minutes, and then replenish the evaporated water;

[0111] (2) Weigh the components of phase B in sequence according to the formula table, and fully dissolve them in boiling water for 15 minutes;

[0112] (3) After the two phases are fully dissolved, cool down to 80°C, slowly pour phase B into phase A, homogenize at 4000r / min for 5 minutes, cool to 45°C, add flavor, preservative and sheep placenta antioxidant polypeptide;

[0113] (4) Add 0.1mol / mL NaOH to adjust the pH of the cream to about 6.5;

[0114] (5) Stand still for 24 hours, vacuumize and r...

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Abstract

The invention provides a sheep placenta antioxidant polypeptide as well as an enzymatic hydrolysis preparation method and an application thereof. With sheep placenta leftovers as raw materials, the sheep placenta polypeptide, which is high in antioxidant activity, is prepared by conducting enzymatic hydrolysis by virtue of papain, then conducting separation and purification by virtue of macroporous adsorption resin, an ultrafiltration membrane, sephadex gel chromatography and semi-preparative RP-HPLC reversed-phase high-performance liquid chromatography, collecting an obtained material and freeze-drying the material, wherein the amino acid sequence of the sheep placenta polypeptide is shown as Glu-Pro-Val-Ser-His-Phe. According to the method provided by the invention, sheep placenta processing wastes can be effectively utilized, so as to improve an added value and increase an economic benefit, and the method has the advantages of being high in product activity, convenient and feasible in preparation process, environment-friendly and the like; with the application of the method provided by the invention, the natural polypeptide, which is high in antioxidant activity, can be obtained, and the prepared polypeptide, replacing an artificially synthesized antioxidant, such as a functional raw material in anti-aging cosmetics, can be used for preparing lotions, night creams, eye creams, gels, essences, facial masks and the like.

Description

technical field [0001] The invention relates to a biological peptide and its preparation method and application, in particular to a sheep placenta antioxidant polypeptide and its enzymolysis preparation method and application. Background technique [0002] Since ancient times, the placenta has been valued by famous doctors of all dynasties for its medicinal value, and is regarded as a treasure of traditional Chinese medicine. Compared with other animal placenta, sheep placenta is most similar to human placenta in structure and composition, and has good nutritional value. Modern medical research has found that sheep placenta contains a large number of bioactive factors such as epidermal growth factor and hyaluronic acid stimulating factor, a variety of enzymes that promote and improve tissue metabolism, 17 kinds of amino acids and 14 kinds of mineral elements. The active substances extracted and purified from sheep placenta by scientific methods are mainly composed of small ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K7/06C07K1/36C07K1/34C07K1/22C07K1/16C07K1/20A61K8/64A61Q19/08A61P17/18
CPCA61K8/64A61K2800/805A61Q19/08C07K7/06C12P21/06
Inventor 李明曹光群杨成姜惠敏
Owner 江苏艾玛仕生物科技有限公司
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