Process for abstracting high-purity hemoglobin from pig blood

Abstract

The invention relates to a method for extracting high-purity hemoglobin from pig blood, and on the condition of 2 to 6 DEG C, the following operations are carried out sequentially: first, anticoagulant pig blood is used for preparing packed red blood cells; hypotonic solution is used for dissolving packed red blood cells, in which inert gas is pumped, antioxidant is added, the pH value of the solution is adjusted and protective agent and antibacterial drugs are added, and after high-speed centrifugation, crude hemoglobin solution is obtained; toluene is added into the crude hemoglobin solution which is extracted in low temperature and the hemoglobin solution in the lower layer is extracted for dialysis; after being frozen and dried the hemoglobin solution after dialysis is then dissolved into high-concentration and small-volume liquid which is then purified by a sephadex chromatography column as a sample and high-concentration hemoglobin with even molecular weight is obtained to be frozen and dried in vacuum after being filtered for removing pyrogen so as to prepare high-concentration hemoglobin dry powder with no matrix or pyrogen. The hemoglobin obtained through the method of the invention has no matrix or pyrogen, which can be further modified to produce blood substitute safely and effectively and the operation is simple; the cost is low.

Description

technical field [0001] The invention relates to a technology for separating and purifying hemoglobin based on blood substitutes, in particular to a method for extracting high-purity hemoglobin from pig blood. Background technique [0002] Blood transfusion is often one of the important means of saving lives. In our country, almost all hospitals in major cities have experienced blood shortages, and some operations have to wait for the arrival of blood before they can be performed. Generally, blood can only be stored for 21 days under low temperature conditions, and there are problems of infection of viruses such as AIDS and hepatitis and matching of blood types. Therefore, the preparation of a safe and effective blood substitute is of great significance to blood transfusion medicine. [0003] The research on blood substitutes has a history of nearly a hundred years, and there are two main research directions: fluorocarbons and hemoglobin Hb products. Fluorocarbons are inco...

AI Technical Summary

Problems solved by technology

Practice has proved that the amount of miscellaneous protein is still relatively large after heating, and some hemoglobin will be denatured and precipitated after heating, and its further ultrafiltration not only increases bacterial and virus contamination, but also increases production costs. The filter membrane is easy to be blocked, and the cost of the ultrafiltration membrane is also high, so it is not easy to expand the production scale

In addition, it is well known that hemoglobin solutions are very unstable and are prone to oxidation and denaturation even at low temperatures

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