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Process for abstracting high-purity hemoglobin from pig blood

InactiveCN101289493AGuaranteed pHReduce the chance of denaturation inactivationPeptide preparation methodsHigh concentrationAntioxidant
The invention relates to a method for extracting high-purity hemoglobin from pig blood, and on the condition of 2 to 6 DEG C, the following operations are carried out sequentially: first, anticoagulant pig blood is used for preparing packed red blood cells; hypotonic solution is used for dissolving packed red blood cells, in which inert gas is pumped, antioxidant is added, the pH value of the solution is adjusted and protective agent and antibacterial drugs are added, and after high-speed centrifugation, crude hemoglobin solution is obtained; toluene is added into the crude hemoglobin solution which is extracted in low temperature and the hemoglobin solution in the lower layer is extracted for dialysis; after being frozen and dried the hemoglobin solution after dialysis is then dissolved into high-concentration and small-volume liquid which is then purified by a sephadex chromatography column as a sample and high-concentration hemoglobin with even molecular weight is obtained to be frozen and dried in vacuum after being filtered for removing pyrogen so as to prepare high-concentration hemoglobin dry powder with no matrix or pyrogen. The hemoglobin obtained through the method of the invention has no matrix or pyrogen, which can be further modified to produce blood substitute safely and effectively and the operation is simple; the cost is low.
Owner:INST OF FIELD OPERATION SURGERY NO 3 MILITARY MEDICL UNIV PLA

Circuit and device for detecting temperature of reference voltage bjt tube

The invention provides a circuit for detecting a reference voltage bjt tube section temperature, which is characterized in that a PNP1 base electrode is connected with a PNP2 base electrode to the ground, and a PNP1 emitting electrode is connected with a first end of an R4; the second end of R4 and the second end of R3 are connected to the drain electrode of MP1; the first end of R3 and the secondend of R1 are connected to the first input end of OP; the emitter of the PNP2 and the second end of the R5 are connected to the drain of the MP2; the first end of R5 and the second end of R2 are connected to the second input end of OP; the grid electrode of the MP1, the grid electrode of the MP2 and the grid electrode of the MP3 are all connected to an OP output end; the source electrode of the MP1, the source electrode of the MP2 and the source electrode of the MP3 are connected with a power supply VDD; the drain electrode of the MP3 is connected with the second end of the R6; the first endof R2, the collector electrode of PNP2, the collector electrode of PNP1, the first end of R1 and the first end of R7 are all connected with a circuit ground GND; the first end R6 and the second end R7are connected with the negative end of the comparator; the voltage VBE1 between the base electrode and the emitting electrode of the PNP1 is transmitted to the positive end VBE2 of the comparator through the switch S1; and an output signal temp _ o of the comparator is connected to the test pad through S2. According to the invention, the temperature-saving working temperature of the BJT tube canbe indicated, so that the temperature of the chip can be indicated and alarmed.
Owner:ETOWNIP MICROELECTRONICS BEIJING CO LTD

Edge grinding machine with dustproof structure for glasses machining

The invention relates to an edge grinding machine with a dustproof structure for glasses machining. The edge grinding machine comprises a machining part and a clamping block, a positioning device is arranged outside the machining part, a supporting and adjusting device is installed at one end of the exterior of the positioning device, a sliding way is installed at one end of the exterior of the supporting and adjusting device, and a hydraulic air rod is arranged at one end of the interior of the sliding way; and a connecting disc is installed outside the hydraulic air rod, a stabilizing device is arranged outside the connecting disc, and a connecting base is installed in the middle of the outer portion of the rear end of the stabilizing device. The edge grinding machine with the dustproof structure for glasses machining has the beneficial effects that the edge grinding machine comprises the machining part and the clamping block, the positioning device is arranged outside the machining part, the supporting and adjusting device is installed at one end of the exterior of the positioning device, the sliding way is installed at one end of the exterior of the supporting and adjusting device, the hydraulic air rod is arranged at one end of the interior of the sliding way, the connecting disc is installed outside the hydraulic air rod, and the stabilizing device is arranged outside the connecting disc.
Owner:海口尹特赛斯智能科技有限公司

A circuit and device for detecting the junction temperature of a reference voltage bjt tube

A circuit for detecting the junction temperature of the reference voltage bjt tube provided by the present invention includes: the base of PNP1 is connected to the base of PNP2 to ground, the emitter of PNP1 is connected to the first end of R4; the second end of R4 and the second end of R3 are connected to the drain of MP1 pole; the first end of R3 and the second end of R1 are connected to the first input end of OP; the emitter of PNP2 and the second end of R5 are connected to the drain of MP2; the first end of R5 and the second end of R2 are connected to the second input end of OP; MP1 gate, MP2 gate and MP3 gate are all connected to OP output terminal; MP1 source, MP2 source and MP3 source are connected to power supply VDD; MP3 drain is connected to the second terminal of R6; R2 first terminal, PNP2 collector , The collector of PNP1 and the first terminal of R1 and the first terminal of R7 are connected to the circuit ground GND; the first terminal of R6 and the second terminal of R7 are connected to the negative terminal of the comparator; the voltage VBE1 between the base and emitter of PNP1 is transmitted through the switch S1 To the positive terminal VBE2 of the comparator; the output signal temp_o of the comparator is connected to the test pad through S2. The invention can indicate the junction temperature working temperature of the BJT tube, thereby indicating and alarming the temperature of the chip.
Owner:ETOWNIP MICROELECTRONICS BEIJING CO LTD

Process for abstracting high-purity hemoglobin from pig blood

InactiveCN101289493BGuaranteed pHReduce the chance of denaturation inactivationPeptide preparation methodsHigh concentrationAntioxidant
The invention relates to a method for extracting high-purity hemoglobin from pig blood, and on the condition of 2 to 6 DEG C, the following operations are carried out sequentially: first, anticoagulant pig blood is used for preparing packed red blood cells; hypotonic solution is used for dissolving packed red blood cells, in which inert gas is pumped, antioxidant is added, the pH value of the solution is adjusted and protective agent and antibacterial drugs are added, and after high-speed centrifugation, crude hemoglobin solution is obtained; toluene is added into the crude hemoglobin solution which is extracted in low temperature and the hemoglobin solution in the lower layer is extracted for dialysis; after being frozen and dried the hemoglobin solution after dialysis is then dissolved into high-concentration and small-volume liquid which is then purified by a sephadex chromatography column as a sample and high-concentration hemoglobin with even molecular weight is obtained to be frozen and dried in vacuum after being filtered for removing pyrogen so as to prepare high-concentration hemoglobin dry powder with no matrix or pyrogen. The hemoglobin obtained through the method of the invention has no matrix or pyrogen, which can be further modified to produce blood substitute safely and effectively and the operation is simple; the cost is low.
Owner:INST OF FIELD OPERATION SURGERY NO 3 MILITARY MEDICL UNIV PLA
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