Ulva fasciata polysaccharide and application thereof

Abstract

The invention discloses ulva fasciata polysaccharide and application thereof. The ulva fasciata polysaccharide is prepared by the following steps: performing an ultrasonic auxiliary water extract and alcohol precipitation process on washed ulva fasciata as a raw material, thereby obtaining crude ulva fasciata polysaccharide; decoloring and removing protein from the crude ulva fasciata polysaccharide by using a radial flow chromatographic column by tan ion exchanger A103S as a packing; separating and purifying the crude ulva fasciata polysaccharide of which the color and the protein are removed by using the radial flow chromatographic column by taking Sephadex-G100 as a packing, thereby obtaining pure ulva fasciata polysaccharide with uniform molecular weight. The method for preparing the ulva fasciata polysaccharide has the characteristics of environment protection, rapidness, high efficiency and low cost, and is applicable to on-scale production; the contents of protein and the pigment in the prepared ulva fasciata polysaccharide are both relatively low, the relatively high purity is achieved, the molecular weight distribution is concentrated, the moisture absorption and retention property is improved, and the result that of ulva fasciata polysaccharide has the shearing thickening property is found for the first time. Therefore, the ulva fasciata polysaccharide disclosed by the invention can be used as a moisture absorption and retention agent or a thickening agent.

Description

(1) Technical field [0001] The present invention relates to the research and development of marine substances, in particular to Ulva sliver polysaccharide and its application, especially its application in moisture absorption and moisturizing, and belongs to the field of marine hydrophilic colloids. (2) Background technology [0002] At present, the most commonly used moisturizers mainly include hyaluronic acid, chitin and its derivatives, etc. The industrial production of hyaluronic acid has the disadvantages of high manufacturing cost and low production efficiency, and chitin cannot be directly dissolved in water, which limits its use in daily chemicals. wide range of applications. Commonly used chemically synthesized moisturizers such as glycerin have remarkable hygroscopicity but average moisturizing properties, and the moisturizing effect will change with changes in the environment. The polysaccharide extracted from natural plants has the characteristics of non-toxic, ...

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Problems solved by technology

The paper has the following technical problems: 1. There is no step of removing protein and pigment, resulting in a certain amount of impurity protein and pigment in the polysaccharide after ultrafiltration, which increases the difficulty in post-processing; 2. The purification method used The method is very complicated. It needs to be diluted, filtered and concentrated separately, and then purified by DEAE-Sepharose Fast Flow ion column and Sephadex-G100 Sephadex column to obtain a pure product with relatively uniform molecular weight; 3. The DEAE-Sepharose Fast used The Flow ion column needs to be eluted with distilled water first, and then eluted with a NaCl gradient of 0.2, 0.4, 1.0 and 2mol / L, and collected in sections, and the eluted polysaccharide contains a considerable concentration of NaCl, and the subsequent Sephadex column needs Desalted and flushed from Sephadex gel with copious amounts of pure water

CN 103483460A discloses a preparation method of Ulva lobula sulfated polysaccharide with anti-tumor activity. The invention discloses a preparation method of Ulva lobata sulfated polysaccharide with anti-tumor activity: pulverize Ulva lobata and extract it with ultrasonic water, Alcohol precipitation to obtain crude polysaccharides, depigmentation and deproteinization with radial flow chromatographic column filled with weakly basic anion exchange resin, after elution, ultrafiltration separation according to molecular weight, and Ulva polysaccharide fraction with molecular weight of 10KD to 30KD Adding a radial flow chromatographic column filled with DEAE agarose gel for enrichment and purification, the prepared polysaccharide with an average molecular weight of 20KD has significant anti-tumor activity, but the polysaccharide is not suitable for use as a thickener and hygroscopic moisturizer

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