Treatment method for enhancing stability of blueberry cyanidin

Abstract

The invention relates to a treatment method for enhancing stability of blueberry cyanidin. The treatment method comprises the following steps: extracting with a 0.5% trifluoroacetic acid (TFA) methanol solution, and sequentially purifying through an ion exchange resin column Amberlite XAD-7 and a Sephadex column Sephadex LH-20 to obtain a blueberry cyanidin refined substance; carrying out nutgall acylation reaction on the blueberry cyanidin refined substance and prepared triacetyl nutgall acyl chloride to introduce galloyl group with ortho-triphenolhydroxy group into the molecular structure, thereby obtaining the modified product nutgall acylated cyanidin. When high-performance liquid chromatography is used for determining the gallic acid amount generated after hydrolyzing the modified product, the nutgall acylation degree is 55-63%. The experiment proves that the stability of the modified product is obviously enhanced. The method for treating blueberry cyanidin is simple to operate, and has the advantages low cost, low pollution and high acylation degree. The molecular modification can enhance the primary characteristics of the blueberry cyanidin, so that the effects are enhanced; and multiple functional groups are introduced to endow the blueberry cyanidin with new physiological activity, thereby widening the application range.

Description

Technical field [0001] The invention relates to a processing method for improving the stability of blueberry anthocyanins. Background technique [0002] Bilberry, a plant belonging to the genus Vaccinium of the azalea family (Eriaceae), is a perennial deciduous or evergreen shrub. The fruit is a blue or red nearly round berry. The blueberry has the largest cultivation area in the world. Commonly known as blueberries. The blueberry fruit is not only attractive in color, but also has delicate meat, moderate sweet and sour, rich nutrition, refreshing aroma and unique flavor. It can be eaten fresh or processed into a variety of foods, and is deeply loved by consumers. Not only that, research has found that blueberries are rich in functional ingredients, such as anthocyanins, trace elements, vitamins, pectin, SOD, etc.; especially, the content of anthocyanins ranks first among all kinds of fruits, which makes blueberries also have health care And drug value. Modern scientific resea...

AI Technical Summary

Problems solved by technology

[0003] Since anthocyanins were extracted and separated, great progress has been made in basic and applied research, but in terms of their practical application, there are still the following problems: (1) The polyhydric water solubility of anthocyanins limits its The application in lipid products, especially as an oil antioxidant, is difficult to reach the effective antioxidant concentration threshold in oil; (2) In terms of human body efficacy, it is difficult to reach the target due to poor fat solubility and difficult to penetrate the double lipid layer cell membrane. to the point of action and greatly reduce its proper activity

In addition, it has poor stability and low effective concentration in physiological environment, resulting in low bioavailability in vivo