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Preparation and application of sephadex surface apigenin molecular engram sorbing material

A technology of dextran gel and molecular imprinting, which is applied in other chemical processes, chemical instruments and methods, etc., can solve the problems of unsolved apigenin selectivity, destruction of imprinted points, poor controllability, etc., and achieve Good physical and chemical stability, strong adsorption capacity and low production cost

Inactive Publication Date: 2012-08-01
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The selectivity of separating and extracting apigenin from complex Chinese herbal medicines and plants has not been effectively resolved
This method requires a simple device and strong universality, but there are usually the following problems: (1) The controllability is poor during the grinding process, and some irregular particles are inevitably generated, and some imprinted spots are destroyed at the same time.
However, there are no published patents and literature reports on the synthesis of apigenin molecularly imprinted adsorption materials using dextran gel as a support.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Dextran gel activation treatment: pre-swelled and washed dextran gel several times in 6mol / L hydrochloric acid solution, soaked at room temperature for 24 hours, washed with deionized water until neutral, and dried at 80°C after suction filtration , to obtain activated dextran gel;

[0020] (2) Acylation treatment of Sephadex: Add 35mL of water, 40mL of tetrahydrofuran, and 10g of activated Sephadex into a four-neck flask, heat the solution at 60°C; add 3.0g of NaOH under stirring, stir evenly, and keep Add 3.0 g of 3-bromopropene dropwise under pressure. After the dropwise addition, keep the temperature at 60°C, stir and react for 5 hours, cool to room temperature, filter, wash with deionized water and methanol, and dry to obtain acylated dextran gel;

[0021] (3) Preparation of apigenin molecularly imprinted adsorption material on the surface of dextran gel: add 11.3mL tetrahydrofuran into a four-neck flask, then add 0.10g apigenin and 1.0g acrylamide, stir and di...

Embodiment 2

[0024] (1) Dextran gel activation treatment: pre-swelled and washed dextran gel several times in 6mol / L hydrochloric acid solution, soaked at room temperature for 24 hours, washed with deionized water until neutral, and dried at 80°C after suction filtration , to obtain activated dextran gel;

[0025] (2) Acylation treatment of Sephadex: Add 35mL of water, 40mL of tetrahydrofuran, and 10g of activated Sephadex into a four-neck flask, heat the solution at 60°C; add 3.0g of NaOH under stirring, stir evenly, and keep Add 3.0 g of 3-bromopropene dropwise under pressure. After the dropwise addition, keep the temperature at 60°C, stir and react for 5 hours, cool to room temperature, filter, wash with deionized water and methanol, and dry to obtain acylated dextran gel;

[0026] (3) Preparation of apigenin molecularly imprinted adsorption material on the surface of dextran gel: add 13.5mL tetrahydrofuran into a four-neck flask, then add 0.15g apigenin and 0.8g acrylamide, stir at roo...

Embodiment 3

[0029] (1) Dextran gel activation treatment: pre-swelled and washed dextran gel several times in 6mol / L hydrochloric acid solution, soaked at room temperature for 24 hours, washed with deionized water until neutral, and dried at 80°C after suction filtration , to obtain activated dextran gel;

[0030](2) Acylation treatment of Sephadex: Add 35mL of water, 40mL of tetrahydrofuran, and 10g of activated Sephadex into a four-neck flask, heat the solution at 60°C; add 3.0g of NaOH under stirring, stir evenly, and keep Add 3.0 g of 3-bromopropene dropwise under pressure. After the dropwise addition, keep the temperature at 60°C, stir and react for 5 hours, cool to room temperature, filter, wash with deionized water and methanol, and dry to obtain acylated dextran gel;

[0031] (3) Preparation of apigenin molecularly imprinted adsorption material on the surface of dextran gel: add 11.5mL tetrahydrofuran to a four-neck flask, then add 0.10g apigenin and 1.0g acrylamide, stir at room t...

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PUM

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Abstract

The invention discloses a preparation method of sephadex surface apigenin molecular engram sorbing material as well as the application of the sephadex surface apigenin molecular engram sorbing material to selective adsorption separation of apigenin molecules in the analysis of foods and medicine. In the method, sephadex is taken as a support, and apigenin molecular engram polymer is decorated on the surface of the sephadex. The invention is characterized in that apigenin, acrylamide, ethylene-glycol dimethyl acrylate, azodiisobutyronitrile and acylation sephadex are added in a certain proportion; and in the medium of furanidine, argon gas is used to remove oxygen, an reaction lasts for 20 to 30 h in thermostatic water bath at the temperature of 60 to 65 DEG C, and then filtering and washing are performed. Acetic acid and methanol solution with the volume fraction of 12 to 18 percent is used for Soxhlet extraction for 12 to 22 h so as to remove apigenin template molecules, and then the material is obtained through washing and drying. The invention has the advantages that a specific recognition capability to apigenin molecules is achieved, the selectivity is high, the adsorbing speed is fast, the adsorbing performance is excellent, the biological degradation is achieved, the process is simple, and regeneration capability and environmental protection are achieved.

Description

technical field [0001] The invention relates to a preparation method and application technology of a molecularly imprinted adsorption material, in particular to a preparation method of an apigenin molecularly imprinted adsorption material on the surface of a dextran gel and its application technology in food testing. Background technique [0002] Apigenin, also known as apigenin, belongs to flavonoids. It has various pharmacological activities such as anti-tumor, anti-inflammation, lowering blood pressure, anti-arteriosclerosis and thrombosis, anti-anxiety, anti-bacterial, anti-virus, and anti-oxidation. It has high research and application value. The selectivity of separating and extracting apigenin from complex Chinese herbal medicines and plants has not been effectively resolved. Therefore, it is of great significance to apply molecularly imprinted polymers with special affinity and selectivity to template molecules in the purification and analysis of traditional Chinese...

Claims

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Application Information

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IPC IPC(8): C08F290/10C08F222/14C08F220/56C08B37/02C08J9/26B01J20/285
Inventor 李慧芝许崇娟李冬梅陈亚明魏琴
Owner UNIV OF JINAN
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