Preparation method of cordyceps militaris polypeptide

A technology of Cordyceps militaris and residues, which is applied in the field of preparation of Cordyceps militaris polypeptides, and achieves the effect of simple operation

Inactive Publication Date: 2016-07-06
HEILONGJIANG JOHNSUN BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] During the extraction process of active ingredients such as cordycepin and cordyceps polysaccharides, most of the residues of the subunit are discarded in the form of residues, resulting in waste of resources and environmental pollution. In the residue of Cordyceps militaris obtained by extracting cordycepin, there will be some residues of cordycepin and cordyceps polysaccharides, which have the functions of anti-oxidation, antibacterial, and improving the body's immunity

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0022] (2) Peptide extraction from the residue of Cordyceps militaris: Take 1.0g of the residue of Cordyceps militaris in an Erlenmeyer flask, add distilled water to adjust the mass concentration of the substrate to 20.0-100.0mg / mL, adjust the pH value of the solution to 6.5 with 1mol / L hydrochloric acid , add 0.01g helicase, hydrolyze at 37℃ for 3h, boil for 10min to inactivate the enzyme; after cooling, use 1mol / L sodium hydroxide to readjust the pH of the solution to 8.0-12.0, add alkaline protease 2-10%, 40- Hydrolyze at 70°C for 5-20 hours, after the hydrolysis is completed, stop the enzymolysis reaction in a boiling water bath for 10 minutes, filter with suction, dry the filter residue to constant weight, and obtain the enzymolysis solution of the residue of Cordyceps militaris;

[0023] (3) Activated carbon decolorization: Accurately measure 30mL of the enzymatic hydrolysis solution of the residue of Cordyceps militaris in a conical flask, adjust the pH value of the solu...

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Abstract

The invention discloses cordyceps militaris polypeptide and a production method thereof. The cordyceps militaris polypeptide is prepared by the following technical steps: extraction of cordyceps militaris protein, extraction of cordyceps militaris extracted residue polypeptide, activated carbon decoloration, separation and purification of the polypeptide and the like. According to the preparation method disclosed by the invention, cordyceps militaris extracted residues, which serve as a raw material, are enzymatically hydrolyzed so as to prepare the polypeptide, then cordyceps militaris HWM residue enzymatic hydrolysate is decolorized by virtue of activated carbon, and finally, the cordyceps militaris polypeptide is separated and purified by virtue of sephadex gel chromatography.

Description

Technical field: [0001] The invention relates to the technical field of extracting Cordyceps militaris polypeptides, in particular to a preparation method of Cordyceps militaris polypeptides. Background technique [0002] Cordyceps militaris is also known as Cordyceps militaris, Cordyceps militaris, Cordyceps militaris, etc. Relevant studies have shown that Cordyceps militaris is rich in three types of nutrients: protein, lipid, and sugar, as well as a variety of vitamins and trace elements, and is rich in cordycepic acid (mannitol ), Cordycepin (3'-deoxyadenosine), Cordyceps polysaccharide and superoxide dismutase (SOD) and other biologically active substances. Cordyceps militaris is a medicinal and edible fungus of the same species as Cordyceps sinensis. Its main medicinal ingredients are the same as those of Cordyceps sinensis. It has the functions of regulating immunity, inhibiting tumors, and delaying aging. [0003] Bioactive peptides are widely used in medicine, food...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/16
Inventor 陈喜军
Owner HEILONGJIANG JOHNSUN BIOLOGICAL ENG CO LTD
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