Preparation method of cordyceps militaris polypeptide
A technology of Cordyceps militaris and residues, which is applied in the field of preparation of Cordyceps militaris polypeptides, and achieves the effect of simple operation
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[0022] (2) Peptide extraction from the residue of Cordyceps militaris: Take 1.0g of the residue of Cordyceps militaris in an Erlenmeyer flask, add distilled water to adjust the mass concentration of the substrate to 20.0-100.0mg / mL, adjust the pH value of the solution to 6.5 with 1mol / L hydrochloric acid , add 0.01g helicase, hydrolyze at 37℃ for 3h, boil for 10min to inactivate the enzyme; after cooling, use 1mol / L sodium hydroxide to readjust the pH of the solution to 8.0-12.0, add alkaline protease 2-10%, 40- Hydrolyze at 70°C for 5-20 hours, after the hydrolysis is completed, stop the enzymolysis reaction in a boiling water bath for 10 minutes, filter with suction, dry the filter residue to constant weight, and obtain the enzymolysis solution of the residue of Cordyceps militaris;
[0023] (3) Activated carbon decolorization: Accurately measure 30mL of the enzymatic hydrolysis solution of the residue of Cordyceps militaris in a conical flask, adjust the pH value of the solu...
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