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Preparation method of wheat germ anti-oxidation peptides

A technology of wheat germ and antioxidant peptides, which is applied in the field of preparation of wheat germ antioxidant peptides, can solve the problem that wheat germ is not fully utilized, achieve good application value, high protein extraction rate, and improve the effect of utilization

Inactive Publication Date: 2018-06-15
HANGZHOU HENGTIAN FLOUR GROUP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, wheat germ is not fully utilized and has potential value for intensive processing

Method used

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  • Preparation method of wheat germ anti-oxidation peptides
  • Preparation method of wheat germ anti-oxidation peptides
  • Preparation method of wheat germ anti-oxidation peptides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Such as figure 1 Shown, the preparation method of wheat germ antioxidant peptide of the present invention is specifically as follows:

[0029] (1) Microwave vacuum drying and superfine grinding of wheat germ to obtain wheat germ superfine powder, take 50 g of a sample passed through a 60-mesh sieve, use 0.5 mol / L sodium chloride, pH value 10, and solid-liquid ratio 1:10 , the sample was leached for 60min by an ultrasonic-assisted method, the ultrasonic wave was 40W, leached twice, and the leaching solution was combined to obtain a wheat germ protein solution;

[0030] (2) adopt 10000rpm centrifugal 30min to carry out solid-liquid separation, get supernatant, be wheat germ protein extract;

[0031] (3) Get 900mL of the wheat germ protein extract obtained in step (2) and carry out ultrafiltration concentration, the temperature is 40°C, the operating pressure is 0.1MPa, the ultrafiltration membrane aperture is 200nm, and concentrated to 260mL to obtain the wheat germ prot...

Embodiment 2

[0046] (1) After the wheat germ is subjected to microwave vacuum freeze-drying and superfine pulverization, the wheat germ superfine powder is obtained, and 50 g of a sample of 60 mesh sieves is taken, 0.8mol / L sodium chloride is used, the pH value is 10, and the solid-liquid ratio is 1: 8. The sample was extracted for 100 minutes by the ultrasonic-assisted method, and the ultrasonic wave was 60W, and the extraction was repeated once to obtain the wheat germ protein solution;

[0047] (2) adopt 10000rpm centrifugal 60min to carry out solid-liquid separation, get supernatant, be wheat germ protein extract;

[0048] (3) Get 800mL of the wheat germ protein extract obtained in step (2) and carry out ultrafiltration and concentration, the temperature is 40°C, the operating pressure is 0.7MPa, the ultrafiltration membrane aperture is 10nm, and concentrated to 300mL to obtain the wheat germ protein concentrate;

[0049] (4) Take 50mL of wheat germ protein concentrate, add pepsin at a...

Embodiment 3

[0054] (1) Microwave vacuum drying and superfine grinding of wheat germ to obtain superfine powder of wheat germ, take 100g of sample through a 60-mesh sieve, use 0.2mol / L sodium chloride, pH value 8, solid-liquid ratio 1:5 , the sample was extracted for 120min by an ultrasonic-assisted method, and the ultrasonic wave was 30W, and the extraction was repeated once to obtain a wheat germ protein solution;

[0055] (2) adopt 10000rpm centrifugal 60min to carry out solid-liquid separation, get supernatant, be wheat germ protein extract;

[0056] (3) Get 950mL of the wheat germ protein extract obtained in step (2) and carry out ultrafiltration concentration, the temperature is 40°C, the operating pressure is 0.1MPa, the ultrafiltration membrane aperture is 200nm, and concentrated to 500mL to obtain the wheat germ protein concentrate;

[0057] (4) adding 0.1% pepsin to the wheat germ protein concentrate, adjusting the pH value to 1.0, incubating at 30°C for 360 minutes, boiling at 1...

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Abstract

The invention discloses a preparation method of wheat germ anti-oxidation peptides. The method comprises the following steps: performing microwave vacuum drying and ultrafine crushing on wheat germs,and adding an alkaline salt solution to extract wheat germ proteins; and separating the obtained extract and raffinate after the extraction, performing ultrafiltration and concentration on the extract, carrying out enzymatic hydrolysis on the obtained concentrate by using pepsin to obtain a wheat germ active peptide solution, performing enzyme deactivation, secondarily concentrating the obtained solution to obtain a wheat germ peptide concentrate, performing separation and purification through Sephadex gel chromatography and ion exchanger chromatography, and drying the obtained solution to obtain the purified wheat germ anti-oxidation peptides. The method allows the protein extraction rate of the wheat germs to be high, and the wheat germ anti-oxidation peptides have good activity and goodapplication values.

Description

technical field [0001] The invention relates to the technical field of food processing, and in particular provides a preparation method of wheat germ antioxidant peptide. Background technique [0002] Wheat germ is one of the main by-products of the wheat processing industry, and it is also the most nutritious part of wheat, accounting for 2 to 3% of the wheat grain. It is rich in protein, carbohydrates, fat, ash, amino acids and some vitamins and other physiologically active ingredients. It is called It is the essence and nutritional treasure house of wheat. Wheat germ protein content is high, up to about 30%, second only to soybeans, contains albumin, gliadin and glutenin, etc., water-soluble protein accounts for more than 30%. The most beneficial component after proteolysis of wheat germ is bioactive peptide, which is a peptide compound that is beneficial to the life activities of organisms or has physiological effects, also known as functional peptide. Active peptides ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/14C07K1/36C07K1/34C07K1/16C07K1/18
CPCC12P21/06C07K1/145C07K1/16C07K1/18C07K1/34C07K1/36
Inventor 蔡成岗邬大江毛建卫李建华沙如意蔡海莺余波
Owner HANGZHOU HENGTIAN FLOUR GROUP
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