Feeding silk antibacterial peptide preparation and preparation method thereof

Abstract

The invention discloses a feeding silk antibacterial peptide preparation and a preparation method thereof. The preparation method comprises the following steps of (1) degumming waste silk and dissolving calcium chloride of the degummed silk; (2) mixing the degummed silk solution prepared by the step (1) with the adopted Alcalase 2.4L and Flavorzyme according to the proportion of 3 to 2, controlling the reaction conditions that the pH is 8.5, the temperature is 55 DEG C and the reaction timeis 5h, and preparing silk polypeptide enzymatic hydrolysate; (3) dialyzing, desalting and concentrating the silk polypeptide enzymatic hydrolysate prepared by the step (2); (4) separating and purifying silk antibacterial peptide through DEAE-52, and Sephadex G-25 sephadex; (5) performing vacuum freeze drying on the silk antibacterial peptide solution prepared by the step (4), and obtaining a finished product. The invention aims to provide a green silk polypeptide preparation process which is used for replacing a feed supplement antibiotics and recovering the waste silk, and is simple in preparation process and easy to clean.

Description

technical field [0001] The invention belongs to the technical field of animal feed additives, and in particular relates to a feed silk antibacterial peptide preparation and a preparation method thereof. Background technique [0002] Xinhuanet reported on November 23, 2015 that a "superbug" gene that can resist powerful antibiotics was recently discovered in livestock and humans, which means that the "last line of defense" in antibiotics used by humans has been broken. risk. The researchers analyzed bacterial samples collected from pork, chicken, and human patients and found that there is a special gene MCR-1, and bacteria carrying this gene show strong resistance to colistin, and this drug resistance can also Quick transfer to other strains. [0003] Due to the abuse of various antibiotics, hormones and illegal drugs to feed livestock and poultry in China, frequent food safety incidents have occurred, and the export of livestock and poultry meat products has declined year ...

AI Technical Summary

Problems solved by technology

[0004] In recent years, people have researched and developed antibiotic substitutes such as feed enzyme preparations, feed microbial preparations, acidifiers, antibacterial peptides, oligosaccharides, allicin, Chinese herbal medicines, and organometallic trace elements. These green feed additives have been favored by people. The favor of these antibiotics is based on the research on the basis of inhibiting the proliferation of harmful bacteria, promoting the proliferation of beneficial bacteria, and improving the intestinal microenvironment. However, in general, these antibiotic substitute products still have certain effects compared with antibiotics. gap

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