Ginseng saponin Rg1 and Rb1 in pseudo-ginseng and preparation of total saponin thereof
A technology of Panax notoginseng saponins and ginsenosides, applied in the field of medicine, can solve the problems of large side effects, limited therapeutic effect and the like
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Embodiment 1
[0031]Take 1 kg of Panax notoginseng medicinal material, add 10 times the amount of water, heat and reflux for 1 hour, filter, repeat the whole process three times, combine the filtrate, and pass the filtrate through a macroporous adsorption resin column (HP-20 wet resin, 1kg) that has been balanced with water in advance, First use water 3 times the weight of the resin to elute the impurities, discard the water solution, then use 20% ethanol 3 times the weight of the resin to elute the impurities, and discard the dilute alcohol solution. Then desorb the saponin with 40% ethanol which is 3 times the weight of the resin, and the eluent is decompressed to recover ethanol, concentrated to an extract, dried in vacuum (70° C.), and pulverized to obtain 25 g of extract (extraction rate: 2.5%). This medicinal extract 10g 50% ethanol ultrasonic dissolving, filters, and filtrate passes through the good ODS C18 post of equilibrium, 30% ethanol eluting, ultraviolet 210nm detects, collects ...
Embodiment 2
[0033] Take 1 kg of Panax notoginseng medicinal material, add 10 times the amount of water, heat and reflux for 1 hour, filter, repeat the whole process three times, combine the filtrate, and pass the filtrate through a macroporous adsorption resin column (HP-20 wet resin, 1kg) that has been balanced with water in advance, First use water 3 times the weight of the resin to elute the impurities, discard the water solution, then use 20% ethanol 3 times the weight of the resin to elute the impurities, and discard the dilute alcohol solution. Then desorb the saponin with 40% ethanol which is 3 times the weight of the resin, and the eluent is decompressed to recover ethanol, concentrated to an extract, dried in vacuum (70° C.), and pulverized to obtain 25 g of extract (extraction rate: 2.5%). This extract 10g 40% ethanol ultrasonic dissolving, filter, filtrate 40% ethanol passes through the LH-20 column that has balanced, 40% ethanol elutes, ultraviolet 210nm detects, collects the e...
Embodiment 3
[0035] Take 1 kg of Panax notoginseng medicinal material, add 10 times the amount of water, heat and reflux for 1 hour, filter, repeat the whole process three times, combine the filtrate, and the filtrate extract is passed through a macroporous adsorption resin column (HP-20 wet resin, 1kg ), first elute the impurities with water 3 times the weight of the resin, discard the aqueous solution, then elute the impurities with 20% ethanol 3 times the weight of the resin, and discard the dilute alcohol solution. Then desorb the saponin with 40% ethanol which is 3 times the weight of the resin, and the eluent is decompressed to recover ethanol, concentrated to an extract, dried in vacuum (70° C.), and pulverized to obtain 25 g of extract (extraction rate: 2.5%). 10 g of this extract was added with chloroform: methanol (4:1) for heating and dissolving, and then passed through a silica gel chromatography column (300 grams), and eluted with a mixed solution of chloroform: methanol (4:1) ...
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