Preparation of aconite primary diterpene alkaloid component and use in disinsection

A technology of diterpene alkaloids and alkaloids, applied in the direction of chemicals, applications, and biocides for biological control, which can solve the problems of less research

Inactive Publication Date: 2009-05-20
SOUTHWEAT UNIV OF SCI & TECH
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, research on the insecticidal activity of diterpene alkaloids in aconite foc...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Raw aconite powder 50kg, leached 4 times with 95% ethanol, each time for a week, combined and recovered ethanol under reduced pressure to obtain extract. Dissolve the extract in water, adjust the pH to 3 with hydrochloric acid, adjust the pH to 10 with alkaline water after degreasing with petroleum ether, then extract with an equal volume of chloroform for 5 times, combine and recover the chloroform to obtain 327.5 g of total alkaloid extract. The total alkaloid extract was separated by column chromatography on a silica gel column, and the chloroform-methanol system was used to roughly separate the total alkaloids at a ratio of 200:1 to 10:1 to obtain alkaloids of different polar segments, a total of 8 parts, a total of 296.2 g. Among them, part 1 and part 2 are subjected to silica gel column chromatography (petroleum ether: ethyl acetate 4:1~1:1), and aconitine, mesoaconitine, and hypoaconitine are obtained through recrystallization; Column chromatography (chloroform-...

Embodiment 2

[0018] Insecticidal activity test of aconitine: according to the evaluation SOP (pesticide volume) of creating pesticide bioactivity, take a certain quality of aconitine with an analytical balance (0.0001g), add 0.1% Tween-80 Solvent (DMF), formulated as a 5% formulation. A certain amount of preparation is weighed, diluted with distilled water (acetone for spot method dilution) to prepare a medicinal solution for measuring the required concentration, and the general screening concentration is selected as 500mg / L.

[0019] The rice seedlings were fixed in a petri dish with white quartz sand, and then CO 2 Anesthetize the mid-third instar nymphs of rice brown planthopper and spray them under the POTTER spray tower. After spraying, cover it with a transparent plastic cup, mark it and put it in the observation room. Check the results after 72 hours.

[0020] Cut off both ends of broad bean leaves, put the back side up on a small piece of cotton, put it in a plastic petri dish, ...

Embodiment 3

[0023] The insecticidal activity test of song Guoling: according to create pesticide biological activity evaluation SOP (insecticide volume), take the song Guoling of certain quality with analytical balance (0.0001g), add the solvent (DMF) that contains 0.1% Tween-80, prepare Into a 5% preparation. A certain amount of preparation is weighed, diluted with distilled water (acetone for spot method dilution) to prepare a medicinal solution for measuring the required concentration, and the general screening concentration is selected as 500mg / L.

[0024] The rice seedlings were fixed in a petri dish with white quartz sand, and then CO 2 Anesthetize the mid-third instar nymphs of rice brown planthopper and spray them under the POTTER spray tower. After spraying, cover it with a transparent plastic cup, mark it and put it in the observation room. Check the results after 72 hours.

[0025] Cut off both ends of broad bean leaves, put the back side up on a small piece of cotton, put i...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for preparing main diterpenoid alkaloids of radix aconiti carmichaeli by means of extraction and separation from crude radix aconiti carmichaeli of the traditional Chinese medicine and a desinsection application thereof, pertaining to the technical field of the preparation of a natural active ingredient and the application thereof. The preparation method for the main diterpenoid alkaloids of the crude radix aconiti carmichaeli comprises the steps that the Mianyang radix aconiti carmichaeli is taken as a raw material which is extracted by ethanol after being dried and ground, then subjected to acid and alkali treatment and is leached by an organic solvent (such as chloroform, acetic ether, and the like) to obtain the general diterpenoid alkaloids; and the general diterpenoid alkaloid obtained is subjected to silica gel column chromatography and separation and then the main diterpenoid alkaloids are obtained through recrystallization. The main diterpenoid alkaloids in radix aconiti carmichaeli are proven to have relatively good desinsection activity by activity tests and can be used for developing pesticides with novel plant sources and also serving as a lead compound and a synthesized material of pesticides to study and create novel pesticides through the structure-activity relationship.

Description

technical field [0001] The invention relates to a method for extracting and separating main diterpene alkaloids of aconite from raw aconite of traditional Chinese medicine and its insecticidal application, and belongs to the technical field of preparation and application of natural active ingredients. Background technique [0002] Aconitum is a well-known commonly used traditional Chinese medicine in my country. The original plant is Aconitum carmichaeli, a plant of Ranunculaceae, which is rich in various diterpene alkaloids such as aconitine, mesoaconitine, and hypoaconitine. It is mainly cultivated in Mianyang, Sichuan Province, with an annual output of about 6 million kg. It has a medicinal history of more than 2,000 years. According to "Shen Nong's Materia Medica", aconite has the effects of restoring yang and rescuing adverse events, dispelling cold and dispelling wind, but because of its It is classified as an inferior drug because of its high toxicity. Modern pharmac...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07D221/22A01N43/42A01N65/32A01P7/00
Inventor 袁小红侯大斌陈亚玲徐春霞贺新生索志荣陈红英
Owner SOUTHWEAT UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap