Method for separating and purifying polysaccharides from winter jujubes

Abstract

The invention relates to a method for separating and purifying polysaccharides from winter jujubes, which comprises the following steps: extracting primary crude polysaccharides from winter jujubes through water extraction and alcohol precipitation processes; carrying out deproteinization treatment on the primary crude polysaccharides through an enzyme-trichloroacetic acid process, and carrying out centrifugal separation to obtain primary supernatant liquid; adding activated carbon into the primary supernatant liquid to carry out decolorizing treatment to obtain secondary supernatant liquid; carrying out membrane filtration on the secondary supernatant liquid to obtain secondary crude polysaccharides; carrying out primary chromatography on the secondary crude polysaccharides on an ion exchange column to obtain polysaccharide DP1 crude products and polysaccharide DP2 crude products; and respectively carrying out secondary chromatography on the polysaccharide DP1 crude products and the polysaccharide DP2 crude products after primary chromatography on a gel chromatography column to obtain polysaccharide DP1 pure products and polysaccharide DP2 pure products. According to the invention, the winter jujube polysaccharides DP1 and DP2 obtained from the winter jujube polysaccharides separated and purified from the winter jujubes have certain antioxidant activity, the product purity is high, the energy consumption is low, and high-quality polysaccharides lay the foundation for better development and utilization in the fields of foods, health products, cosmetics and the like in the future.

Description

technical field [0001] The invention relates to the technical field of polysaccharide extraction, in particular to a method for separating and purifying polysaccharides from jujube. Background technique [0002] Dongzao (Zizyphus jujubecv. Dongzao) is a plant of the Rhamnaceae Jujube genus, which is the fruit of the jujube tree (Zizyphus jujubecv), and is a kind of late-maturing fresh jujube unique to my country. It is mainly produced in Shandong, Hebei and other places. At present, the planting area in northern Shandong has exceeded several million mu, so it is also called Lubei Dongzao. Because of its beautiful fruit shape, bright color, crisp and juicy flesh, winter jujube is rich in 19 kinds of amino acids and multivitamins needed by the human body, and also rich in calcium, potassium, iron, zinc, copper and other minerals and trace elements And more medicinal substances, such as polysaccharides, flavonoids, cyclic adenosine monophosphate, etc., have high therapeutic va...

AI Technical Summary

Problems solved by technology

At present, domestic and foreign research reports on winter jujube mainly focus on the cultivation and management of jujube trees, fruit storage and processing, and pest control. There are few research reports on the extraction, separation and purification process of winter jujube polysaccharides. Currently, there are reports on the extraction process of winter jujube polysaccharides. Mainly hot water extraction, ultrasonic extraction, microwave extraction and microwave-assisted enzyme extraction, but the extracted polysaccharide contains a large amount of protein, pigment and other small molecular impurities, the product has low purity and high energy consumption

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