Lactobacillus plantarum SR9-3 with functions of reducing cholesterol and antioxidant activity and application thereof

Abstract

The invention discloses Lactobacillus plantarum SR9-3 with functions of reducing cholesterol and antioxidant activity. The Lactobacillus plantarum SR9-3 is characterized in that a lactic acid bacterium separated from traditional fermented sour meat of minority nationalities in Guizhou goes through an antioxidation test and is identified, and an identification result is that the lactic acid bacterium is Lactobacillus plantarum SR9-3, the Lactobacillus plantarum SR9-3 is preserved in CCTCC (china center for type culture collection) on September 26, 2016, and the preservation No. is CCTCC No: M 2016529. The Lactobacillus plantarum SR9-3 is a functional lactic acid bacterium and has good feature in the aspects of reducing cholesterol and antioxidant activity, the removal rate of cholesterol of an egg yolk source is 21.98%, and the removal rate of cholesterol in a culture medium with 1% of lecithin is 31.84%; in the aspect of antioxidation, the total reducing capacity is 24.62%, the eliminating ability of DPPH and the eliminating ability of OH are 46.32% and 257.92% respectively, and the anti-lipid peroxidation ability is 67.13%. The strain has potential in developing a probiotics strain and is applied in health-care food or fermented food.

Description

technical field [0001] The invention relates to a microorganism, and further to a strain of lactobacillus plantarum (Lactobacillus plantarum) SR9-3 capable of lowering cholesterol and anti-oxidation function, and its screening method and industrial application. Background technique [0002] Medical research has proved that elevated serum cholesterol levels in humans are considered to be one of the most important risk factors for cardiovascular diseases such as atherosclerosis and coronary heart disease. Clinical studies have also shown that the normal serum cholesterol level is 5.20mmol / L, 1mmol more than the normal value, the risk of coronary heart disease increases by 35%, and reducing the serum cholesterol level by 1% may reduce the risk of disease by 2%-3%. In addition, reactive oxygen species and free radicals can cause abnormal metabolism in the body, resulting in some diseases. [0003] Probiotics are a class of microorganisms that can improve the microbial balance o...

AI Technical Summary

Problems solved by technology

Despite these applications, only single reference to cholesterol-lowering or anti-oxidant antioxidant function

[0007] However, only CN201610182616.4 "A Strain of Lactobacillus plantarum Effectively Removing Cholesterol and Free Radicals and Its Application" is the only patent application involving Lactobacillus plantarum capable of lowering cholesterol and scavenging free radicals, but it does not involve acid production performance, resistance to In terms of osmotic pressure characteristics, antibacterial properties, and anti-oxidation, this application does not involve the test of total reducing ability and anti-oxidation ability. The cholesterol determination method in this application adopts the P-Fe-S reagent colorimetric method

Studies have reported that the P-Fe-S method is affected by many factors, and the detection results are unstable

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