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Lactobacillus paracasei subsp. paracasei and application thereof

A paracheese and lactobacillus technology, applied in the field of microorganisms, can solve problems such as carcinogenicity and liver damage, and achieve the effect of good salt tolerance

Inactive Publication Date: 2020-06-19
FOSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are a variety of antioxidants on the market, such as tert-butyl hydroxyanisole, 2,6-di-tert-butyl p-cresol, tert-butyl hydroquinone and butylated hydroxytoluene, etc. However, studies have found that these synthetic Antioxidants have certain damage to the liver and are carcinogenic

Method used

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  • Lactobacillus paracasei subsp. paracasei and application thereof
  • Lactobacillus paracasei subsp. paracasei and application thereof
  • Lactobacillus paracasei subsp. paracasei and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Isolation and identification of Lactobacillus paracasei subspecies CGMCC NO.17893

[0020] 1. Isolation and screening of strains

[0021] Weigh 25g of soy sauce residue samples in a conical flask under aseptic conditions, add 225mL of sterile saline, shake slightly to prepare 10 -1 Diluent. Prepare 10 sequentially -2 ~10 -5 Diluents of equal concentration. Pipette 0.1 mL of soy sauce residue dilutions with different gradients and spread them on the MRS medium. Seal with a parafilm, label with relevant information, and incubate at a constant temperature of 37°C for 24-48 hours until colonies grow.

[0022] Pick suspected lactic acid bacteria colonies and purify them in three sections of MRS medium, and operate 2 to 3 times until the colonies on the medium have the same morphology. The purified strain was inoculated in MRS liquid medium, cultured in a 37°C incubator for 24h, mixed with 50% glycerol 1:1, and stored in a -80°C refrigerator. The screened Lact...

Embodiment 2

[0042]Example 2 Study on the growth characteristics of Lactobacillus paracasei subspecies CGMCC 17892

[0043] (1) Determination of growth curve

[0044] The Lactobacillus paracasei subspecies CGMCC NO.17893 bacterial strain screened out in the above-mentioned embodiment 1 is activated for three generations, cultivated at 37°C for 40h in MRS liquid medium, and measured the strain OD in every 2 hours 600 nm value.

[0045] The result is as image 3 As shown, it can be seen that the Lactobacillus paracasei subspecies CGMCC NO.17893 screened in embodiment 1 has good growth activity.

[0046] (2) Salt tolerance research

[0047] Prepare salt-containing MRS medium with 9 gradients of salt mass fractions of 0-9%, and sterilize it for use. The Lactobacillus paracasei subspecies CGMCC NO.17893 bacterial strain screened out in the above-mentioned embodiment 1 was activated for three generations, cultivated at 37°C for 24h in MRS liquid medium, and measured OD 600 nm value, the r...

Embodiment 3

[0050] Example 3: Research on the probiotic properties of Lactobacillus paracasei subspecies CGMCC17893

[0051] (1) Simulated gastric juice tolerance test

[0052] Activate the Lactobacillus paracasei subspecies CGMCC NO.17893 strain screened in Example 1 for three generations, draw 3mL of culture solution, centrifuge at 4000r / min for 10min at room temperature, remove the supernatant, obtain thallus, and suspend the thallus Prepare bacterial suspension in 3mL sterilized normal saline for later use.

[0053] Add artificial gastric juice and bacterial suspension (9:1) into a sterile centrifuge tube and mix well. Incubate at 37°C, at 0h (dilution of 10 -4 、10 -5 、10 -6 ) and 3h (dilution of 10 -3 、10 -4 、10 -5 ) to take a sample of 0.1 mL, and calculate the survival rate of the strains using the plate count method. The experiment was set up in triplicate.

[0054] Survival rate = (A 1 / A 0 )×100%

[0055] Note: A 1 is the surviving number of lactic acid bacteria tha...

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Abstract

The present invention provides a lactobacillus paracasei subsp. paracasei. The lactobacillus paracasei subsp. paracasei is deposited in the China General Microbiological Culture Collection Center andhas deposit number of CGMCC NO.17893. The lactobacillus paracasei subsp. paracasei has certain antioxidant capacity, can remove DPPH and hydroxyl radicals, also has good salt resistance, acid resistance, bile salt resistance, gastric juice resistance, intestinal juice resistance, and hydrophobicity, thus increases chances of playing a probiotic role, and can be used in fields of fermented feeds, fermented food, health-care food, etc.

Description

technical field [0001] The disclosure relates to the technical field of microorganisms, in particular to a Lactobacillus paracasei subspecies and an application thereof. Background technique [0002] Biological oxidation is a vital process in the activities of living organisms. The oxidation process will generate free radicals, but excessive free radicals will cause oxidative stress. Oxidative stress is the imbalance between pro-oxidative components (such as superoxide, hydrogen peroxide, etc.) and antioxidant components (superoxide dismutase, catalase, glutathione, etc.) in the cells of the body. Oxygen free radicals are generated too much in the body and cannot be removed in time, causing excessive oxidation of the body and causing a series of adaptive reactions that damage the body's cells and tissue functions. This reaction can accelerate the aging of the body and cause related diseases. [0003] Eliminating excessive oxygen free radicals in the body is one of the ways...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23C9/123A23K10/12C12R1/225
CPCA23C9/1234A23K10/12C12N1/20C12N1/205C12R2001/225
Inventor 黄桂东孙张乐区锡敏钟先锋魏梓晴蒋木培
Owner FOSHAN UNIVERSITY
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