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Lactobacillus plantarum capable of degrading biogenic amines and salt tolerance and application thereof

A Lactobacillus plantarum, biogenic amine technology, applied in the directions of Lactobacillus, microorganism-based methods, applications, etc., can solve the problems of inability to degrade biogenic amines, food flavor or quality impact, increased toxicity of histamine and tyramine, etc. Excellent probiotic properties, good salt tolerance, good growth and reproduction

Active Publication Date: 2022-08-05
SOUTHWEST UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that the intake of an appropriate concentration of biogenic amines can promote growth, enhance metabolic activity, strengthen the intestinal immune system, control blood pressure and eliminate free radicals, etc., but biogenic amines are also precursors of carcinogens and nitrous substances. If it is too high, it will have a toxic effect on the human body, causing cardiovascular disease and nervous system damage.
Although cadaverine itself has low toxicity, it can inhibit histamine and tyramine metabolizing enzymes and enhance the toxicity of histamine and tyramine
[0005] At present, the control of biogenic amines in production generally adopts methods such as ultra-high pressure treatment, use of food additives, and low-temperature storage. These methods can reduce the content of biogenic amines in food to a certain extent, but cannot degrade the biogenic amines that have already been produced, and these methods will cause affect the flavor or quality of the food

Method used

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  • Lactobacillus plantarum capable of degrading biogenic amines and salt tolerance and application thereof
  • Lactobacillus plantarum capable of degrading biogenic amines and salt tolerance and application thereof
  • Lactobacillus plantarum capable of degrading biogenic amines and salt tolerance and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Identification of Lactobacillus plantarum SHY610 and biogenic amine degradation and salt tolerance experiments

[0030] 1 Experimental material

[0031] Lactic acid bacteria were isolated and screened from traditional fermented yak yogurt collected from herdsmen's homes in Hongyuan County, Aba Tibetan and Qiang Autonomous Prefecture, Sichuan Province.

[0032] 2 Experimental methods

[0033] 2.1 Strain identification test

[0034] The lactic acid bacteria stored in ampoules were inoculated into MRS broth, and after activation for two generations, plate streaking and Gram staining were performed. If the strain was pure, the bacterial genome DNA extraction kit was used to extract the DNA of the strain. Using bacterial universal primers 27F (5'-AGA GTT TGA TCC TGG CTC AG-3', SEQ ID No. 1) and 1492R (5'-TAC GGY TACCTT GTT ACG ACT T-3', SEQ ID No. 2), The 16S rDNA was amplified by PCR in a 25 μL reaction system, and detected by agarose gel electrophoresis after ...

Embodiment 2

[0085] Embodiment 2 Lactobacillus plantarum SHY610 inoculum is used for the preparation of pepper sprouts

[0086] Preparation of Lactobacillus plantarum SHY610 inoculum: take the activated culture solution in the MRS broth in Example 1, centrifuge at 4 000 r / min for 10 min, discard the supernatant, and wash the collected cell precipitate with sterile saline to obtain active bacteria 1 mL of lyophilized protective agent (10% skimmed milk+0.1% sodium glutamate) was added to the obtained bacterial slurry per 10 mL of culture solution, placed in an ultra-low temperature refrigerator and taken out after quick freezing, and vacuum freeze-dried to obtain Lactobacillus plantarum SHY610 inoculum.

[0087] Remove impurities and inedible parts from 50 parts by weight of prickly ash sprouts, wash the sediment and dirt with clean water, and use a stainless steel tunnel-type steam-killing equipment for steam greening, so that the green prickly ash sprouts (seedlings) are thoroughly killed a...

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Abstract

The invention discloses a salt-tolerant Lactobacillus plantarum capable of degrading biogenic amines, a bacterial agent containing the Lactobacillus plantarum, and their applications in degrading biogenic amines and in preparing fermented foods; The Lactobacillus plantarum is preserved in the China Center for Type Culture Collection, the preservation address is Wuhan City, Hubei Province, and the preservation number is CCTCC NO: M 2019757. The Lactobacillus plantarum SHY610 can degrade the biogenic amines in food with high degradation rate, and the degradation rates of cadaverine, histamine and tyramine reach 62.42%, 74.32% and 89.97% respectively; SHY610 also has good salt tolerance, It can also grow and reproduce well in an environment with a salt content of up to 9.00%, and is also suitable for the production of fermented food with a high salt content.

Description

technical field [0001] The invention relates to the technical field of microorganisms and food fermentation, in particular to a salt-tolerant Lactobacillus plantarum capable of degrading biogenic amines and applications thereof. Background technique [0002] Traditional yak yogurt is made by herdsmen in the Qinghai-Tibet Plateau using yak milk through traditional natural fermentation. It is a special food eaten by herdsmen in the Qinghai-Tibet Plateau. It has a long history. After a long period of natural selection, some of them have probiotic properties and good quality. of bacteria were retained. Yak milk is not only rich in vitamins, but also contains 8 essential amino acids needed by the human body, and is rich in lactic acid bacteria resources. [0003] Lactic acid bacteria is a general term for a class of bacteria that are beneficial to the host and can ferment carbohydrates to produce large amounts of lactic acid. Studies have shown that probiotic lactic acid bacter...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23C9/123A23L11/50A23L13/40A23L17/00A23L19/20A23L27/50C12R1/25
CPCC12N1/20A23C9/123A23L19/20A23L27/50A23L17/65A23L13/45C12R2001/25C12N1/205A23V2400/169
Inventor 索化夷曾维友周於强
Owner SOUTHWEST UNIV
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