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Lactobacillus paracasei SLP16 for producing polysaccharides, application of lactobacillus paracasei SLP16 and feed additive prepared from lactobacillus paracasei SLP16

A lactobacillus and paracheese technology, applied in the directions of lactobacillus, bacteria used in food preparation, application, etc., can solve the problems of low nutritional value, decreased immunity, bacterial drug resistance, etc., and achieve excellent extracellular production of polysaccharides, The effect of improving performance and promoting digestion and absorption

Active Publication Date: 2022-01-04
SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the long-term abuse of antibiotics in feed will lead to excessive veterinary drug residues in animal-sourced foods, resulting in bacterial resistance, decreased animal immunity, and the occurrence of animal and poultry infectious diseases and insect pests.
Therefore, researching feed additives that can replace antibiotics has become the general trend in this field! The unreasonable compatibility of additives used to replace antibiotics in the prior art results in low nutritional value and single efficacy

Method used

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  • Lactobacillus paracasei SLP16 for producing polysaccharides, application of lactobacillus paracasei SLP16 and feed additive prepared from lactobacillus paracasei SLP16
  • Lactobacillus paracasei SLP16 for producing polysaccharides, application of lactobacillus paracasei SLP16 and feed additive prepared from lactobacillus paracasei SLP16
  • Lactobacillus paracasei SLP16 for producing polysaccharides, application of lactobacillus paracasei SLP16 and feed additive prepared from lactobacillus paracasei SLP16

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preparation example Construction

[0036] The present invention also provides a preparation method of the feed additive, comprising the steps of:

[0037] (1) Mix the auxiliary material with water in a weight ratio of 1 to 2:1, preferably 1.5:1, to obtain the material to be inoculated;

[0038] (2) Inoculate the seed culture solution of Lactobacillus paracasei SLP16 in the material to be inoculated, and cultivate it to obtain a feed additive.

[0039] In the present invention, when the seed culture liquid of Lactobacillus paracasei SLP16 is inoculated in the described material to be inoculated, the volume ratio of the seed culture liquid of material to be inoculated and Lactobacillus paracasei SLP16 is 9~10:1, is preferably 9.5: 1;

[0040] The bacterium concentration of Lactobacillus paracasei SLP16 in the seed culture liquid of described Lactobacillus paracasei SLP16 is 0.5~1.5×10 8 CFU / mL, preferably 1×10 8 CFU / mL;

[0041] The temperature of the cultivation is 36-38°C, preferably 37°C;

[0042] The cul...

Embodiment 1

[0075] Passage Stability Study

[0076] The screened Lactobacillus paracasei SLP16 was used as the first-generation strain, and the first-generation strain was used as a seed to inoculate in the MRS solid medium for cultivation to obtain the second-generation strain, and the second-generation strain was used as a seed to inoculate in the MRS solid medium Cultivate the 3rd generation strain, and so on until the 10th generation strain is obtained. The antibacterial test was carried out by double-layer agar plate method. In the mixed bacterial suspension of Escherichia coli and Staphylococcus aureus added during the experiment, the bacterial concentration of Escherichia coli was 10 6 CFU / mL, the bacterial concentration of Staphylococcus aureus is 10 6 CFU / mL. During the experiment, the concentration of bacteria added to each well was 10 8 CFU / mL Lactobacillus paracasei SLP16 culture solution 200 μL, diffused at room temperature for 30 minutes, and cultured at 37° C. for 12 ho...

Embodiment 2

[0082] Antibacterial test

[0083] Adopt the double-layer agar plate method of embodiment 1 to carry out antibacterial test. Observe the inhibitory effect of Lactobacillus paracasei SLP16 on Escherichia coli and Staphylococcus aureus, observe whether there is an inhibition zone around the hole, measure its diameter with a vernier caliper, and record the data. The inhibitory effects of Lactobacillus paracasei SLP16 on Escherichia coli and Staphylococcus aureus were obtained. The results are shown in Table 2.

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Abstract

The invention provides lactobacillus paracasei SLP16 for producing polysaccharides, application of the lactobacillus paracasei SLP16 and a feed additive prepared from the lactobacillus paracasei SLP16, and relates to the technical field of feed additives. The lactobacillus paracasei SLP16 disclosed by the invention is Lactobacillus paracasei SLP16; the Latin name is Lactobacillus paracasei, the name of the preservation unit is Guangdong Microbial Culture Collection Center; the address is Floor 5, Building 59, No. 100 courtyard, Xianlie Middle Road, Guangzhou city; the preservation date is July 5, 2021; and the preservation number is GDMCCNo: 61769. The SLP16 disclosed by the invention is still stable after passage, can inhibit escherichia coli and staphylococcus aureus, has good cholate-resistant and acid-resistant effects, has cohesiveness, hydrophobicity and oxidation resistance, and also can generate exopolysaccharides.

Description

technical field [0001] The invention relates to the technical field of feed additives, in particular to a polysaccharide-producing Lactobacillus paracasei SLP16 and its application and a feed additive prepared by using the strain. Background technique [0002] Adding antibiotics to feed is an important measure for traditional animal husbandry to prevent livestock diseases and improve breeding efficiency. However, the long-term abuse of antibiotics in feed will lead to excessive veterinary drug residues in animal-sourced foods, leading to bacterial resistance, decreased animal immunity, and the occurrence of animal diseases and insect pests. Therefore, researching feed additives that can replace antibiotics has become the general trend in this field! The compatibility of additives used to replace antibiotics in the prior art is unreasonable, resulting in low nutritional value and single efficacy. [0003] Therefore, there is an urgent need for a material that is rich in nut...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
CPCA23K10/12A23K10/37A23V2400/165Y02P60/87
Inventor 赵兴秀胡容何义国赵长青方春玉刘苑皓
Owner SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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