Composition for treating colitis, medicine and preparing method thereof

A technology of colitis, composition, applied in the field of medicine

Active Publication Date: 2016-02-24
大庆仕合源乳业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are reports in the literature that probiotics can treat ulcerative colitis, there is no

Method used

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  • Composition for treating colitis, medicine and preparing method thereof
  • Composition for treating colitis, medicine and preparing method thereof
  • Composition for treating colitis, medicine and preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0057] (4) Preparation of crude extract of bovine colostrum growth factor

[0058] A crude extract of bovine colostrum growth factor prepared by centrifugal degreasing, acid precipitation of casein, secondary ultrafiltration, dialysis and freeze-drying of bovine colostrum; processing methods such as centrifugation, ultrafiltration, dialysis, and drying referred to in the present invention Any method known to those of ordinary skill in the art can be used as long as it can maintain the effect of growth factors. Preferably, centrifugation is performed by centrifugation at 4°C, and freeze-drying is used for drying.

[0059] (5) Preparation of drugs for treating colitis

[0060] The gavage dose of crude bovine colostrum growth factor extract is 5.4mg / 20g (mass of bovine colostrum growth factor crude extract / mouse mass); the gavage form of crude bovine colostrum growth factor extract is, take out and dissolve Lactobacillus acidophilus and Lactobacillus plantarum sludge tubules in 0.1 mL ...

Example Embodiment

[0085] Example 1

[0086] A medicine for treating colitis, comprising 12 parts by volume of probiotics and 64 parts by mass of bovine colostrum growth factor. The bovine colostrum growth factor is a crude growth factor extract extracted from bovine colostrum; the probiotics are Lactobacillus acidophilus CGMCC10436 and Lactobacillus plantarum CGMCC9961, and the quantity ratio of the two is 1:1, And are in the form of live bacteria. Said live bacterial cells are bacterial sludge collected by centrifugation of Lactobacillus liquid cultures that are all in the initial stage of stability. Wherein, parts by volume / parts by mass=ml / mg, and the following examples are the same.

[0087] Moreover, the preparation method of the above-mentioned medicine for treating colitis includes the following steps:

[0088] (1) Culture Lactobacillus acidophilus CGMCC10436 and Lactobacillus plantarum CGMCC9961 in MRS medium respectively;

[0089] (2) Select Lactobacillus acidophilus CGMCC10436 and Lactobac...

Example Embodiment

[0093] Example 2

[0094] A medicine for treating colitis, comprising 16 parts by volume of probiotics and 44 parts by mass of bovine colostrum growth factor. The bovine colostrum growth factor is a crude growth factor extracted from bovine colostrum; the probiotics are Lactobacillus acidophilus CGMCC10436 and Lactobacillus plantarum CGMCC9961, and the quantity ratio of the two is 1:1 , And are in the form of live bacteria. Said live bacterial cells are bacterial sludge collected by centrifugation of Lactobacillus liquid cultures that are all in the initial stage of stability.

[0095] Wherein, the preparation method of the medicine for treating colitis includes the following steps:

[0096] (1) Culture Lactobacillus acidophilus CGMCC10436 and Lactobacillus plantarum CGMCC9961 in MRS medium respectively;

[0097] (2) Select Lactobacillus acidophilus CGMCC10436 and Lactobacillus plantarum CGMCC9961 cultured for 16 hours;

[0098] (3) Take 11 volume parts of Lactobacillus acidophilus C...

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Abstract

The invention discloses a composition for treating colitis, medicine and a preparing method thereof. According to the composition for treating colitis, probiotics and bovine coloctrum growth factors are used in cooperation. Balb/c mice are used as test objects, a colitis model is set up, and the colitis resisting effect of the medicine is studied from the aspects of the apparent state, the body weight change, the colon length, the pathology state and the like of the test mice. The test results show that the effect of the medicine is better than that of medicine singly using the probiotics or the bovine coloctrum growth factors; the the probiotics and the bovine coloctrum growth factors have a cooperative synergism effect, the medicine has a good colitis resistant effect, colitis will not relapse within a short term, and intestinal mucosa injuries caused by colitis can be well repaired.

Description

technical field [0001] The invention relates to a composition for treating colitis, a medicine and a preparation method thereof, that is, a medicine used in combination with two strains of probiotics and bovine colostrum growth factor, and belongs to the technical field of medicine. Background technique [0002] Colitis, also known as non-specific ulcerative colitis, has a wide variety of causes, and the onset is slow and the severity of the disease varies. The main clinical manifestations are diarrhea, abdominal pain, mucus and bloody stools, tenesmus, and even constipation , Can not pass stool within a few days, sometimes diarrhea and sometimes constipation, often accompanied by weight loss and fatigue, etc., often recurrent. Abdominal pain is generally dull pain or colic, often located in the left lower abdomen or lower abdomen. Other manifestations include loss of appetite, abdominal distension, nausea, vomiting, and hepatomegaly. There may be tenderness in the left low...

Claims

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Application Information

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IPC IPC(8): A61K35/747A61P1/00A61P1/04A61K35/20
CPCA61K35/20A61K35/747A61K2300/00
Inventor 霍贵成徐敏靳妲
Owner 大庆仕合源乳业有限公司
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