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Composition for treating colitis, medicine and preparing method thereof

A technology of colitis, composition, applied in the field of medicine

Active Publication Date: 2016-02-24
大庆仕合源乳业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are reports in the literature that probiotics can treat ulcerative colitis, there is no report on the combination of probiotics and bovine colostrum growth factor in the treatment of colitis

Method used

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  • Composition for treating colitis, medicine and preparing method thereof
  • Composition for treating colitis, medicine and preparing method thereof
  • Composition for treating colitis, medicine and preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0057] (4) Preparation of Bovine Colostrum Growth Factor Crude Extract

[0058] The bovine colostrum growth factor crude extract obtained by centrifugal defatting of bovine colostrum, acid precipitation of casein, secondary ultrafiltration, dialysis and freeze-drying; the processing methods such as centrifugation, ultrafiltration, dialysis and drying referred to in the present invention , can use any method known to those of ordinary skill in the art, as long as it can maintain the efficacy of growth factors, preferably, centrifugation at 4°C, and freeze-drying for drying.

[0059] (5) preparation of medicine for the treatment of colitis

[0060] Crude extract of bovine colostrum growth factor, intragastric dosage is 5.4mg / 20g (quantity of crude extract of bovine colostrum growth factor / mass of mouse); the form of infusion of crude extract of bovine colostrum growth factor is, taken out and dissolved in Lactobacillus acidophilus in 0.1 mL of sterile PBS solution, Lactobacillu...

Embodiment 1

[0086] A medicine for treating colitis, comprising 12 parts by volume of probiotics and 64 parts by mass of bovine colostrum growth factor. The bovine colostrum growth factor is a crude extract of growth factors extracted from bovine colostrum; the probiotics are Lactobacillus acidophilus CGMCC10436 and Lactobacillus plantarum CGMCC9961, and the quantitative ratio of the two is 1:1, And all are in the form of live bacteria. The living bacteria cells are respectively centrifuged and collected bacteria sludge from liquid cultures of Lactobacillus in the initial stage of stability. Wherein, parts by volume / parts by mass=ml / mg, and the following examples are the same.

[0087] And, the preparation method of the above-mentioned medicine for the treatment of colitis, comprises the following steps:

[0088] (1) respectively culture Lactobacillus acidophilus CGMCC10436 and Lactobacillus plantarum CGMCC9961 in MRS medium;

[0089] (2) Lactobacillus acidophilus CGMCC10436 and Lactoba...

Embodiment 2

[0094] A medicine for treating colitis, comprising 16 parts by volume of probiotics and 44 parts by mass of bovine colostrum growth factor. Wherein the bovine colostrum growth factor is a crude extract of growth factors extracted from bovine colostrum; the probiotics are Lactobacillus acidophilus CGMCC10436 and Lactobacillus plantarum CGMCC9961, and the quantitative ratio of the two is 1:1 , and are in the form of live bacteria. The living bacteria cells are respectively centrifuged and collected bacteria sludge from liquid cultures of Lactobacillus in the initial stage of stability.

[0095] Wherein, the preparation method of the medicine for the treatment of colitis comprises the following steps:

[0096] (1) respectively culture Lactobacillus acidophilus CGMCC10436 and Lactobacillus plantarum CGMCC9961 in MRS medium;

[0097] (2) Lactobacillus acidophilus CGMCC10436 and Lactobacillus plantarum CGMCC9961 were respectively selected and cultured for 16 hours;

[0098] (3) T...

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Abstract

The invention discloses a composition for treating colitis, medicine and a preparing method thereof. According to the composition for treating colitis, probiotics and bovine coloctrum growth factors are used in cooperation. Balb / c mice are used as test objects, a colitis model is set up, and the colitis resisting effect of the medicine is studied from the aspects of the apparent state, the body weight change, the colon length, the pathology state and the like of the test mice. The test results show that the effect of the medicine is better than that of medicine singly using the probiotics or the bovine coloctrum growth factors; the the probiotics and the bovine coloctrum growth factors have a cooperative synergism effect, the medicine has a good colitis resistant effect, colitis will not relapse within a short term, and intestinal mucosa injuries caused by colitis can be well repaired.

Description

technical field [0001] The invention relates to a composition for treating colitis, a medicine and a preparation method thereof, that is, a medicine used in combination with two strains of probiotics and bovine colostrum growth factor, and belongs to the technical field of medicine. Background technique [0002] Colitis, also known as non-specific ulcerative colitis, has a wide variety of causes, and the onset is slow and the severity of the disease varies. The main clinical manifestations are diarrhea, abdominal pain, mucus and bloody stools, tenesmus, and even constipation , Can not pass stool within a few days, sometimes diarrhea and sometimes constipation, often accompanied by weight loss and fatigue, etc., often recurrent. Abdominal pain is generally dull pain or colic, often located in the left lower abdomen or lower abdomen. Other manifestations include loss of appetite, abdominal distension, nausea, vomiting, and hepatomegaly. There may be tenderness in the left low...

Claims

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Application Information

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IPC IPC(8): A61K35/747A61P1/00A61P1/04A61K35/20
CPCA61K35/20A61K35/747A61K2300/00
Inventor 霍贵成徐敏靳妲
Owner 大庆仕合源乳业有限公司
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