Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Double-protein fermented milk beverage rich in active Lactobacillus plantarum and preparation method thereof

A technology of Lactobacillus plantarum and fermented milk, which is applied to Lactobacillus, bacteria used in food preparation, dairy products, etc., can solve the problem of lack of probiotic characteristics and flavor homogeneity, low concentration of Lactobacillus plantarum live bacteria, and influence of dairy flavor. and other problems to achieve the effect of shortening fermentation time, facilitating absorption and utilization, and improving stability

Active Publication Date: 2018-11-30
JIANGNAN UNIV
View PDF5 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] We can try to use Lactobacillus plantarum instead of Streptococcus thermophilus and Lactobacillus bulgaricus to produce double-protein fermented milk to solve the problems of lack of probiotic properties and serious flavor homogeneity in existing double-protein fermented milk and double-protein fermented milk drinks. However, due to the lack of protease genes of Lactobacillus plantarum, the macromolecular protein in milk cannot be effectively used for growth and reproduction, making this attempt unsuccessful.
[0007] Although Guangming Company's "Changyou" milk beverage uses Lactobacillus plantarum for fermented milk fermentation, it mainly ferments by mixing Lactobacillus plantarum and Lactobacillus bulgaricus and other bacteria. The concentration of viable bacteria is very low, and the fermentation time is very long; the patent with the publication number CN102715325A achieves the purpose of proliferating Lactobacillus plantarum by adding yeast powder as a proliferation factor in the double-protein fermentation system, but the fermentation time is still relatively long. Long, and the yeast powder used in it will have a greater impact on the flavor of dairy products

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Double-protein fermented milk beverage rich in active Lactobacillus plantarum and preparation method thereof
  • Double-protein fermented milk beverage rich in active Lactobacillus plantarum and preparation method thereof
  • Double-protein fermented milk beverage rich in active Lactobacillus plantarum and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Specific steps are as follows:

[0060] (1) Accurately weigh 24.00g skimmed milk powder, 4.00g soybean protein isolate, 1.00g soybean peptone, 0.0004g manganese citrate (12% skim milk powder, 2% soybean protein, 0.5% soybean peptone, 0.0002% manganese citrate ), add purified water to it to measure to 200g, after dissolving, homogenize the emulsion under the condition of 4000r / min, sterilize at 90°C for 15min, cool to 37°C, and obtain the double protein fermentation base material;

[0061] (2) The lyophilized powder of Lactobacillus plantarum CGMCC No.6077 was mixed with 1×10 7 The inoculum amount of cfu / g is added to the fermentation base material under aseptic conditions, and the inoculated fermentation base material is placed in a constant temperature incubator at 37°C for fermentation to obtain fermented milk;

[0062] (3) Stabilizers, sweeteners and flavors were mixed according to the ratio in Table 1, added to pure water preheated to 90°C, stirred quickly, mixed e...

Embodiment 2

[0071] Specific steps are as follows:

[0072] (1) Accurately weigh 24.00g skimmed milk powder, 4.00g soybean protein isolate, 1.00g debittered soybean peptide, 0.0004g manganese citrate (skimmed milk powder 12%, soybean protein 2%, debittered soybean peptide 0.5%, lemon Manganese acid manganese acid 0.0002%), add pure water therein and quantify to 200g, after dissolving, the emulsion is homogenized under the condition of 4000r / min, sterilized at 90°C for 15min, cooled to 37°C, to obtain the double protein fermentation base material;

[0073] (2) The lyophilized powder of Lactobacillus plantarum CGMCC No.5494 was mixed with 1×10 7 The inoculum amount of cfu / g is added to the fermentation base material under aseptic conditions, and the inoculated fermentation base material is placed in a constant temperature incubator at 37°C for fermentation to obtain fermented milk;

[0074] (3) Stabilizers, sweeteners and flavors were mixed according to the ratio in Table 3, added to purifi...

Embodiment 3

[0082] Specific steps are as follows:

[0083] (1) Accurately weigh 24.00g skimmed milk powder, 4.00g soybean protein isolate, 1.00g casein hydrolyzate, 0.0004g manganese citrate (skim milk powder 12%, soybean protein 2%, casein hydrolyzate 0.5%, lemon Manganese acid manganese acid 0.0002%), add pure water therein and quantify to 200g, after dissolving, the emulsion is homogenized under the condition of 4000r / min, sterilized at 90°C for 15min, cooled to 37°C, to obtain the double protein fermentation base material;

[0084] (2) The lyophilized powder of Lactobacillus plantarum CCTCCM 206032 was mixed with 6×10 6 cfu / g is added to the fermentation base material under aseptic conditions, and the inoculated fermentation base material is placed in a constant temperature incubator at 37°C for fermentation to obtain fermented milk;

[0085] (3) Stabilizers, sweeteners and flavors were mixed according to the ratio in Table 4, added to purified water preheated to 90°C, stirred quickl...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a double-protein fermented milk beverage rich in active Lactobacillus plantarum and a preparation method thereof, and belongs to the technical field of fermentation engineering. The preparation method includes the following steps: inoculating Lactobacillus plantarum into double-protein base material components with the addition of proliferating factors and inorganic salts for fermentation to obtain fermented milk, and then adding stabilizers, sweeteners and water into the fermented milk for blending to obtain the fermented milk beverage; the preparation method can successfully realize the proliferation and fermentation of the Lactobacillus plantarum in the double-protein base material, and can greatly shorten the fermentation time of the Lactobacillus plantarum in the double-protein base material; the fermented milk beverage obtained by the preparation method is high in probiotic content, and the viable count can reach 5.0 x 10 8(superscript) cfu / mL or above; regarding the fermented milk beverage, the product system is stable, the protein content meets the requirements of national standards, sourness and sweetness are palatable, and the cost can be controlled; and the fermented milk beverage obtained by the preparation method is performed with low temperature storage at 0-10 DEG C for 21 days, and the viable count can be stabilized at 3.0 x 10 7(superscript) cfu / mL or above.

Description

technical field [0001] The invention relates to a double-protein fermented milk drink rich in active Lactobacillus plantarum and a preparation method thereof, belonging to the technical field of fermentation engineering. Background technique [0002] As a kind of milk product, fermented milk drink is deeply loved by people for its unique sweet and sour taste and frankincense flavor, and has been sold well in the market for many years. According to the types of fermented raw materials, fermented milk drinks can be roughly divided into plant protein fermented milk, milk protein fermented milk drinks and double protein fermented milk drinks. [0003] Among them, the double-protein fermented milk drink is obtained by blending raw milk (or reconstituted milk), white sugar, and soybean protein as the main raw materials and fermented by lactic acid bacteria. Since the soybean protein used in the double-protein fermented milk Contains 8 kinds of essential amino acids needed by the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A23C9/13
CPCA23C9/1315A23C9/1322A23V2400/169
Inventor 陈卫崔树茂丁晓栋毛丙永陆文伟翟齐啸赵建新张灏
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products