Compound antifreeze agent and application thereof
An anti-freezing agent and anti-freezing polypeptide technology, which is applied in the preservation of meat/fish with chemicals, etc., can solve the problems of reduced gel performance, poor flavor, and quality deterioration, and achieves increased added value, prevention of oxidative loss, The effect of increasing the absorption of calcium
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Embodiment 1
[0032] The raw materials and formula of a kind of fish ball added with compound antifreeze agent are as follows: fish meat: 1kg, potato starch: 50g, salt: 20g, monosodium glutamate: 4g, ginger juice: 4g, pepper powder: 2g, antifreeze polypeptide: 40g , Trehalose: 28g, polyphosphate 2.5g (tripolyphosphate / pyrophosphate=1:1).
[0033] The production steps are as follows:
[0034] (1) Preparation of antifreeze polypeptide: take food-source pigskin collagen as raw material, soak collagen and distilled water for 3 hours at a ratio of 1:100 to make the collagen fully absorb water, add enzyme and substrate at a ratio of 1:50 Alkaline protease, adjust the pH of the enzymatic hydrolysis environment to pH9.0, heat and hydrolyze the collagen in a water bath shaker at 37°C, and the enzymatic hydrolysis time is 3h. After enzymatic hydrolysis, put it in a boiling water bath for 10 minutes to completely inactivate the protease. After cooling to room temperature, use a high-speed refrigerate...
Embodiment 2
[0049] The raw materials and formula of a simulated crab meat added with compound antifreeze agent are as follows: fish meat: 1kg, crab meat 0.2kg, potato starch: 65g, salt: 25g, monosodium glutamate: 2g, ginger juice: 5g, pepper powder: 2g , Antifreeze polypeptide: 40g, trehalose: 30g, polyphosphate 3g (tripolyphosphate / pyrophosphate=1:1).
[0050] The production steps are as follows:
[0051] (1) Preparation of antifreeze polypeptide: take food-source pigskin collagen as raw material, soak collagen and distilled water for 3 hours at a ratio of 1:100 to make the collagen fully absorb water, add enzyme and substrate at a ratio of 1:50 Alkaline protease, adjust the pH of the enzymatic hydrolysis environment to pH9.0, heat and hydrolyze the collagen in a water bath shaker at 37°C, and the enzymatic hydrolysis time is 3h. After enzymatic hydrolysis, put it in a boiling water bath for 10 minutes to completely inactivate the protease. After cooling to room temperature, use a high-...
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