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Spirulina gamma-linolenic acid extractive and preparation method thereof

A technology of extract and spirulina, which is applied in the field of spirulina gamma-linolenic acid extract and its preparation, can solve the problems of high equipment requirements, urea residue, and no significant increase in extraction rate

Active Publication Date: 2012-08-15
ORDOS XINYULI SPIRULINA IND GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the former has high requirements on equipment, and the extraction rate has not been significantly improved; the latter has urea residue, which is easy to form suspected carcinogen urethane, which is not ideal for direct use in food and health products

Method used

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  • Spirulina gamma-linolenic acid extractive and preparation method thereof
  • Spirulina gamma-linolenic acid extractive and preparation method thereof
  • Spirulina gamma-linolenic acid extractive and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Mix 5g of dried spirulina powder with 60mL of ethanol thoroughly, and first use ultrasonic waves in an ice-water bath to break the cells, with a power of 400W and a break time of 10 minutes. The crushed algae powder-ethanol suspension was continuously leached in a constant temperature water bath in the dark, kept at 70°C, oscillated for 90min, and rotated at 150r / min. The γ-linolenic acid extract mixture solution obtained by leaching was first vacuum filtered to remove the residue, and the clarified solution was transferred to a rotary evaporator (175Mbar, 40°C) for vacuum concentration to remove excess solvent. The concentrated extract is vacuum freeze-dried to finally obtain the white powdery Spirulina gamma-linolenic acid extract.

[0019] Take 10mL of pure extract and methylate it with concentrated sulfuric acid-methanol (1:9, v / v) in a constant temperature water bath at 70°C for 10min, add 1mL of n-hexane after cooling and distilled water to make up volume, mix wel...

Embodiment 2

[0021] Fully mix 20Kg of dried spirulina powder with 250L of ethanol, and first use ultrasonic waves in a water bath at 0-40°C to disrupt cells with a power of 400W and a crushing time of 20 minutes (broken in four stages, 5 minutes each time). The crushed algae powder-ethanol suspension was continued to be extracted at a constant temperature and protected from light, keeping the temperature at 70°C, shaking for 90min, and rotating at a speed of 150r / min. The γ-linolenic acid extract mixed solution obtained by leaching is first vacuum filtered to remove the residue, and the clarified liquid is transferred to a double-effect low-temperature vacuum concentration tank for vacuum concentration, thereby removing excess solvent. The concentrated extract is vacuum freeze-dried to finally obtain the white powdery Spirulina gamma-linolenic acid extract.

[0022] Take 10mL of pure extract and methylate it with concentrated sulfuric acid-methanol (1:9, v / v) in a constant temperature wate...

Embodiment 3

[0024] The extracts in Example 1 were subjected to DPPH free radical scavenging test, lipid oxidation inhibition test and platelet aggregation inhibition test respectively.

[0025] DPPH free radical scavenging experiment: Add 50 μL of samples with different concentrations to 5 mL of 0.004% DPPH (dissolved in methanol), measure the absorbance value at 517 nm after 30 min at room temperature, and select Vc as a positive control. The results are shown in Table 1.

[0026] Lipid Oxidation Inhibition Assay: Lecithin for Fe 2+ The mediated oxidation reaction provides a lipid system, and the absorbance value is measured at 535nm with thiobarbituric acid. When the GLA extract is added to the reaction environment, if it exhibits antioxidant activity, the corresponding absorbance changes linearly with the concentration. The results are shown in Table 2.

[0027] Platelet aggregation inhibition test: Fresh pig blood was centrifuged to prepare platelet-rich plasma (PRP) and platelet-po...

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Abstract

The invention discloses a spirulina gamma-linolenic acid extractive and a preparation method of the spirulina gamma-linolenic acid extractive. The spirulina gamma-linolenic acid extractive has high antioxidation activity and antiplatelet activity. According to the spirulina gamma-linolenic acid extractive, spirulina powder is taken as a raw material; and by ultrasonic processing, organic solvent extraction, vacuum filtration, vacuum concentration, vacuum freezing drying and gas chromatograph-mass spectrometer measurement, the powdered spirulina gamma-linolenic acid extractive can be finally obtained. A DPPH (1.1-diphenyl-2-1picryhydrazyl radial) scavenging test, a lipid oxidation inhibition test and a platelet aggregation inhibition test show that the spirulina gamma-linolenic acid extractive has high antioxidation activity and antiplatelet activity, and finally prove that the mode of extracting gamma-linolenic acid from spirulina is an economic and effective mode. The method disclosed by the invention can be applied to the field of development of health-care food.

Description

technical field [0001] The invention relates to a spirulina gamma-linolenic acid extract with high antioxidant activity and antiplatelet aggregation activity and a preparation method thereof, belonging to the fields of modern food processing and biotechnology. Background technique [0002] Gamma-linolenic acid is known as "the protagonist of functional food in the 21st century", and it is an indispensable polyunsaturated fatty acid in the human body. γ-linolenic acid, as the structural material of biological membranes of various tissues, is also the precursor of prostaglandins synthesized by the human body. It has various important physiological activities such as anti-lipid oxidation, anti-atherosclerosis, lowering serum cholesterol concentration, and enhancing immune function. Function. [0003] Recent studies have found that "the most ideal food in the 21st century" recommended by the Food and Agriculture Organization of the United Nations - Spirulina is the only organis...

Claims

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Application Information

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IPC IPC(8): A23L1/30A23L1/337A61K36/02A61P7/02A61P39/06A23L17/60
Inventor 任迪峰苗苗鲁军王菲孙冰洁
Owner ORDOS XINYULI SPIRULINA IND GRP
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