Method for increasing production of astaxanthin in haematococcus pluvialis by mature spore inoculation and iron ion-mediated Harber-Weiss reaction at high temperature

A technology of Haematococcus pluvialis and astaxanthin, applied in biochemical equipment and methods, microorganisms, single-cell algae, etc., can solve problems such as high energy costs

Active Publication Date: 2016-08-31
KOREA UNIV RES & BUSINESS FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in this case, there are disadvantages in that a reactor with a special configuration is required, and high energy costs are required to irradiate a large amount of light
In addition, KR2010-0105193A discloses a method of producing astaxanthin by irradiation, but there is a problem that irradiation costs are significant

Method used

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  • Method for increasing production of astaxanthin in haematococcus pluvialis by mature spore inoculation and iron ion-mediated Harber-Weiss reaction at high temperature
  • Method for increasing production of astaxanthin in haematococcus pluvialis by mature spore inoculation and iron ion-mediated Harber-Weiss reaction at high temperature
  • Method for increasing production of astaxanthin in haematococcus pluvialis by mature spore inoculation and iron ion-mediated Harber-Weiss reaction at high temperature

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1: Effects of various high-temperature culture conditions and iron ion-mediated Haber-Weiss reaction on the accumulation of astaxanthin and biomass in Haematococcus pluvialis under autotrophic conditions

[0063] To examine the effects of various high-temperature culture conditions and iron ion-mediated Haber-Weiss reaction on the accumulation of astaxanthin and biomass in Haematococcus pluvialis under autotrophic conditions, the following experiments were performed.

[0064] In NIES-C medium lacking an organic carbon source, low-intensity light (20 μE / m 2 / s) and only carbon dioxide as a single carbon source for culturing cells. When the exponential phase was reached, the cells in the medium (OD 680 = about 0.8) was transferred to NIES-N medium to inhibit the growth of cells, and the amount of biomass and the degree of accumulation of astaxanthin were analyzed for 18 days under each of the following conditions: general room temperature culture conditions (23 °...

Embodiment 2

[0072] Example 2: Intracellular reactive oxygen species content, SOD in the initial stage of astaxanthin synthesis in Haematococcus pluvialis under various high temperature culture conditions and iron ion-mediated Haber-Weiss reaction under autotrophic conditions Effects of Activity, Lipid Oxidation and Carotenoid Content

[0073] In order to examine the inhibition of astaxanthin biosynthesis in Haematococcus pluvialis at high temperature under autotrophic conditions, the following experiments were performed.

[0074] First, low-intensity light (20 μE / m 2 / s) and only carbon dioxide as a single carbon source for culturing cells. When the exponential phase was reached, the cells in the medium (OD 680 = about 0.8) was transferred to NIES-N medium to inhibit the growth of cells, and intracellular reactive oxygen species content (DCF content), SOD activity was analyzed during 2 days of culture in each of the following astaxanthin synthesis conditions , lipid oxidation degree (M...

Embodiment 3

[0078] Example 3: O at room temperature (23°C) under autotrophic conditions 2 - Effects of the artificial generation and iron-mediated Haber-Weiss reaction on intracellular astaxanthin accumulation in the initial stage of astaxanthin synthesis in Haematococcus pluvialis

[0079] To examine LROS (O 2 - and H 2 o 2 ) to increase the inhibition of astaxanthin synthesis, the following experiments were carried out. In NIES-C medium lacking an organic carbon source, low-intensity light (20 μE / m 2 / s) and only carbon dioxide as a single carbon source for culturing cells. When the exponential phase was reached, the cells in the medium (OD 680 = about 0.8) was transferred to NIES-N medium to inhibit cell growth, and various concentrations (10 -11 to 10 -7 M) methyl viologen together with strong light (150μE / m 2 / s) added to cells to artificially generate O 2 - . In addition, the degree of astaxanthin accumulation in the cells was analyzed at 4 days of culture under each of...

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Abstract

The present invention relates to a method for increasing the production of astaxanthin in Haematococcus pluvialis by a two-stage light cultivation process and, more specifically, to a method for increasing the production of astaxanthin in Haematococcus pluvialis cells by inoculating mature spores (cyst) and adding iron ions under high-temperature autotrophic conditions. The two-stage light cultivation process for the production of astaxanthin under autotrophic conditions using the sun during outdoor cultivation comprising inoculating mature spore (cyst) cells and adding iron ions, according to the present invention, can solve the problem that synthesis of astaxanthin is inhibited by excess generation of LROS(O2-, H2O2) by effectively converting LROS(O2-, H2O2) that is generated in a large amount under high-temperature conditions to MROS(O2, OH.) and amplifying an intracellular lipid oxidation signal, thereby more economically increasing the production of astaxanthin. Such astaxanthin is useful in a variety of industrial fields as a potent antioxidant.

Description

technical field [0001] The present invention relates to a method for increasing astaxanthin production in Haematococcus pluvialis by a two-stage photocultivation method, and more specifically, to A method for increasing astaxanthin production in Haematococcus pluvialis by adding iron ions. Background technique [0002] Generally, the red ketocarotenoid astaxanthin (3,3'-dihydroxy-β,β'-carotene-4,4'-dione) is a kind of A carotenoid pigment of chemical structure equivalent to that of a carotenoid, and is an antioxidant substance having the ability to remove toxic reactive oxygen species. Astaxanthin has significantly higher antioxidant activity than those of existing antioxidant substances because it has a unique molecular structure compared to β-carotene, which has an extra hydroxyl group at both ends group (-OH) and ketone group (═O). The antioxidant activity of astaxanthin is about 500 times that of vitamin E, a representative antioxidant, and about 20 times that of β-ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P23/00C12N1/12C12N13/00
CPCC12N1/12C12N13/00C12P23/00
Inventor 沈相俊洪珉义
Owner KOREA UNIV RES & BUSINESS FOUND
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