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Preparation method of Beauveria sp. extract with functions of tyrosinase inhibitor and antioxidant

An antioxidant and tyrosinase technology, applied in the direction of microorganism-based methods, preparation of sugar derivatives, chemical instruments and methods, etc., can solve the problems of application limitations, unstable properties, high production costs, etc., and achieve a wide range of uses, The effect of low product cost and high success rate

Inactive Publication Date: 2012-01-25
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the fact that some of the natural antioxidants found so far have high production costs and some are not stable

Method used

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  • Preparation method of Beauveria sp. extract with functions of tyrosinase inhibitor and antioxidant
  • Preparation method of Beauveria sp. extract with functions of tyrosinase inhibitor and antioxidant
  • Preparation method of Beauveria sp. extract with functions of tyrosinase inhibitor and antioxidant

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] The operation steps of the preparation method of the Beauveria bassiana extract with tyrosinase inhibitor and antioxidant effects are as follows:

[0058] 1.1. Selection and cultivation of Beauveria bassiana strains

[0059] Beauveria bassiana used was Beauveria bassiana ( Beauveria bassiana );

[0060] The specific cultivation method is as follows:

[0061] 1.1.1 Inclined seed culture

[0062] The preserved Beauveria bassiana strains were inoculated on SDAY slant medium, kept at a constant temperature of 20°C, and cultured for 4 days to obtain the first-grade strains;

[0063] 1.1.2 Secondary seed cultivation

[0064] Inoculate the primary strains into the liquid shake flask culture medium,

[0065] Liquid shake flask medium: glucose 40 g / L, peptone 10 g / L, yeast extract powder 10 g / L, distilled water to volume;

[0066] Add 30% of the volume of the Erlenmeyer flask into a liquid medium larger than 100 ml, inoculate each Erlenmeyer flask with 1 slant strain, an...

Embodiment 2

[0094] The operation steps of the preparation method of the Beauveria bassiana extract with tyrosinase inhibitor and antioxidant effects are as follows:

[0095] 1.1. Selection and cultivation of Beauveria bassiana strains

[0096] The Beauveria bassiana used was Beauveria bassiana ( Beauveria brongniartii );

[0097] The specific cultivation method is as follows:

[0098] 1.1.1 Inclined seed culture

[0099] The preserved Beauveria bassiana strains were inoculated on SDAY slant medium, kept at a constant temperature of 30°C, and cultured for 7 days to obtain the first-grade strains;

[0100] 1.1.2 Secondary seed cultivation

[0101] Inoculate the primary strains into the liquid shake flask culture medium,

[0102] Liquid shake flask medium: glucose 40 g / L, peptone 10 g / L, yeast extract powder 10 g / L, distilled water to volume;

[0103] Add 50% of the volume of the liquid medium in the 1000 ml Erlenmeyer flask, inoculate 3 slant strains into each Erlenmeyer flask, place...

Embodiment 3

[0131] The operation steps of the preparation method of the Beauveria bassiana extract with tyrosinase inhibitor and antioxidant effects are as follows:

[0132] 1.1. Selection and cultivation of Beauveria bassiana strains

[0133] The Beauveria bassiana used was Beauveria bassiana ( Beauveria amorpha );

[0134] The specific cultivation method is as follows:

[0135] 1.1.1 Inclined seed culture

[0136] The preserved Beauveria bassiana strains were inoculated on SDAY slant medium, kept at a constant temperature of 25°C, and cultured for 5 days to obtain the first-grade strains;

[0137] 1.1.2 Secondary seed cultivation

[0138] Inoculate the primary strains into the liquid shake flask culture medium,

[0139] Liquid shake flask medium: glucose 40 g / L, peptone 10 g / L, yeast extract powder 10 g / L, distilled water to volume;

[0140] Add 40% of the volume of the Erlenmeyer flask into a 250 ml Erlenmeyer flask, and inoculate each Erlenmeyer flask with 1 slant strain. After...

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Abstract

The invention relates to a preparation method of Beauveria sp. extract with functiosn of a tyrosinase inhibitor and an antioxidant. The preparation method comprises the following operation steps: 1. selecting and culturing Beauveria sp. Strain; and 2. extracting and refining active components from a fermented product, wherein the refining process comprises primary refining and chromatographic refining. The Beauveria sp. and Beauveria sp. extract provided by the invention have tyrosinase inhibiting and antioxidation active effects, and the refined product has the minimum IC50 (50% inhibiting concentration) of 134.5 mu g/ml to tyrosinase and the minimum IC50 of 87.2 mu g/ml to rat liver cell lipid oxidation; the Beauveria sp. extract can be used as articles for daily use to take into effects of whitening, antioxidation, antiaging, preserving, sterilizing, anti-inflammatory and the like; because the Beauveria sp. extract is produced by microorganism fermentation, the production of the Beauveria sp. extract is not influenced by environment and natural resources, and is easy to industrialize and automatize; the process method has low production cost, simple process, stable process, and high success rate and is easy to adjust and control; and the preparation method is suitable for investment of enterprises in various scales.

Description

technical field [0001] The invention belongs to the technical field of extraction and preparation of biological products, and relates to a preparation method of a Beauveria bassiana extract with functions of tyrosinase inhibitor and antioxidant. Background technique [0002] Beauveria bassiana ( Beauveria sp. ) classification: Hyphospora ( Hyphomycetales ) Beauveria bassiana ( Beauveria Vuillemin) fungi; there are three common types of this genus in my country, such as: Beauveria bassiana ( Beauveria bassiana ), Beauveria bassiana ( Beauveria brongniartii ) and Beauveria bassiana ( Beauveria amorpha ), etc., are important entomopathogenic fungi, and are currently mainly used for biological control of pests. Wherein the silkworm is called Beauveria bassiana after being infected with Beauveria bassiana. The role of white silkworm in traditional Chinese medicine in my country is mainly "expelling wind and relieving spasm, resolving phlegm and resolving stagnation"....

Claims

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Application Information

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IPC IPC(8): C12P19/44C07H15/203C07H1/06C12R1/645
Inventor 胡丰林陆瑞利黄勃李增智
Owner ANHUI AGRICULTURAL UNIVERSITY
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