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Method for extracting antioxidation activity small peptide nutrient powder from wheat embryo

An antioxidant activity, wheat germ technology, applied in plant protein processing, food preparation, food science and other directions, can solve the problems of high cost of small peptides and high cost of raw materials, and achieve the effect of large development value and low cost

Inactive Publication Date: 2009-08-26
周靖波 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are a series of techniques for extracting small peptides, such as extracting small peptides from heparin slag and extracting small peptides from Pinus tabulaeformis pollen (patent application number 200710058028.0), but the cost of raw materials for these methods is relatively high, and the product small peptides high cost

Method used

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  • Method for extracting antioxidation activity small peptide nutrient powder from wheat embryo

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: (1) superfinely pulverize wheat germ to 400 meshes; (2) use lactic acid as a solvent to adjust the pH value to 3.5, and then use deionized water to prepare a wheat germ suspension with a solid content of 1%; ( 3) the non-protein components in the wheat germ suspension are extracted and removed; (4) the wheat germ protein liquid after the non-protein is removed is put into a container with a stirrer, and the solid content of the wheat germ protein liquid is adjusted with deionized water. The content is 1%, then use ammonia water to adjust the pH value to 6.5, add neutral protease, the amount of neutral protease is 0.003% of the total weight of the mixture before adding, control the temperature at 20°C, stir for 24 hours, the activity of neutral protease ≥200,000 u / g; (5) Add r-transpeptidase, the amount of r-transpeptidase is 0.001% of the total weight of the mixture before adding, stir at 20°C for 10 hours, and then centrifuge to remove the residue, That i...

Embodiment 2

[0017] Example 2: (1) superfinely pulverize wheat germ to 500 mesh; (2) use citric acid as a solvent to adjust the pH value to 3.8, and then use deionized water to prepare a wheat germ suspension with a solid content of 4%; (3) the non-protein components in the wheat germ suspension are extracted and removed; (4) the wheat germ protein liquid after the non-protein is removed is put into a container with a stirrer, and the deionized water is used to regulate the content of the wheat germ protein liquid The solid content is 4%, then adjust the pH value to 4.5 with dodecyldimethylbenzyl ammonium chloride, add acid protease, the amount of acid protease is 0.5% of the total weight of the mixture before it is added, and control the temperature at 40°C , stirred for 15 hours, acid protease activity ≥ 200,000 u / g; (5) add r-transpeptidase, the amount of r-transpeptidase is 0.5% of the total weight of the mixture before it is added, and stir at 40 ° C for 7 hours, and then centrifuged ...

Embodiment 3

[0018] Embodiment 3: (1) superfinely pulverize wheat germ to 600 mesh; (2) use lactic acid as a solvent to adjust the pH value to 4.0, and then use deionized water to prepare a wheat germ suspension with a solid content of 8%; ( 3) the non-protein components in the wheat germ suspension are extracted and removed; (4) the wheat germ protein liquid after the non-protein is removed is put into a container with a stirrer, and the solid content of the wheat germ protein liquid is adjusted with deionized water. The content is 8%, then use ammonia water to adjust the pH value to 7, add compound protease, the dosage of compound protease is 2% of the total weight of the mixture before adding, control the temperature at 50°C, stir for 10 hours, the activity of compound protease is ≥ 400,000 u / g; (5) Add r-transpeptidase again, the consumption of r-transpeptidase is 2% of the total weight of the mixed solution before it is added, stir at 50°C for 4 hours, then centrifuge to remove the sla...

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Abstract

The invention provides a method for extracting antioxidation activity small peptide nutrient powder from wheat embryo. The method comprises the following steps: nonprotein of the wheat embryo is removed by extraction after the wheat embryo is crushed; then, prolease and peptidase are added into the wheat embryo to obtain small peptide nutrient fluid; finally, the small peptide nutrient fluid is concentrated and dried to obtain the peptide nutrient powder. The main nutrient ingredient in the prepared small peptide nutrient powder is antioxidation activity small peptide (karnosin), wherein content of dipeptide is over 56.4 percent; and the small peptide can inhibit lipid oxidation catalyzed by iron, hemoglobin, lipid oxidase and singlet oxygen in vitro, and can reduce autoxidation rate and content of fatty hydrogen peroxide, thereby playing a role of oxidation resistance of the small peptide nutrient powder. The small peptide nutrient powder is a perfect product of resisting age and preventing obese, and is mainly used for foods and medicine additives.

Description

technical field [0001] The invention relates to a method for extracting antioxidant active small peptide nutritional powder, in particular to a method for extracting antioxidant active small peptide nutritional powder from wheat germ. Background technique [0002] Wheat bran and wheat germ are the main by-products of flour mills and have historically been used as feed. Modern research shows that wheat germ is the essence of wheat grains, accounting for 3-5% of the total wheat. Wheat germ contains a variety of nutritional active ingredients, such as protein, amino acid, vitamin E, fat, minerals and dietary fiber It is an important health food raw material and an important source of plant protein nutrition, and has a good development and utilization prospect. With the advancement of technology, the application of wheat germ has been various, such as making wheat germ oil from wheat germ. [0003] Classical nutrition believes that protein cannot be directly absorbed and utili...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J3/18A23L1/185A23L1/305A23L1/28A23L33/00A23L33/185
Inventor 周靖波黄继红杨公明
Owner 周靖波
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