Copper metal organic framework nanoparticle functionalized hydrogel, preparation method and application thereof

An organic framework and nanoparticle technology, applied in the field of medical devices, can solve the problems of unfavorable wound healing, low hydrogel strength and water swelling rate, and easy breakage, so as to protect from external damage, promote wound healing, and have good anti-aging properties. Oxidative effect

Active Publication Date: 2021-01-22
SOUTH CHINA UNIV OF TECH
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  • Claims
  • Application Information

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Problems solved by technology

[0003] Chinese invention patent application CN201610219873.0 discloses a preparation method of a medical temperature-sensitive hydrogel containing nano-silver and its application in the field of wound healing. It is composed of nano-silver and chitosan, but due to the strong cytotoxicity of nano-silver, In the process of sterilization, normal cells will be damaged, and the temperature-sensitive hydrogel has poor drug slow-release performance, which will easily cause the burst release of nano-silver, which is not conducive to wound healing; prior art (Copper-nanoparticle-embedded hydrogel forkilling bacteria and promoting wound healing with photothermal therapy, Tao B, Lin C, Deng Y, et al. Copper-nanoparticle-embedded hydrogel for killing bacteria and promoting wound healing with photothermal therapy [J]. Journal of Materials Chemistry B, 2019, 7.) disclosed a Copper nanoparticles (Cu NPs) chelated with methacrylate-modified gelatin (Gel-MA) and N,N-bis(acryloyl)cystamine (BACA) chelated by free-radical polymerization with photoinitiator to prepare composite water Gel, used in medical dressings to promote the healing of infected wounds, this composite hydrogel showed significant antibacterial effects on both Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria, but due to water The gel strength and water swelling rate are low, and it is easily broken after absorbing wound exudate, so it cannot meet the requirements of wound care

Method used

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  • Copper metal organic framework nanoparticle functionalized hydrogel, preparation method and application thereof
  • Copper metal organic framework nanoparticle functionalized hydrogel, preparation method and application thereof
  • Copper metal organic framework nanoparticle functionalized hydrogel, preparation method and application thereof

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Experimental program
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Embodiment 1

[0040] 1) Preparation of glutathione-modified carboxymethyl chitosan (CMCS-GSH): Dissolve 0.5 g carboxymethyl chitosan ( CMCS) to make a homogeneous solution; 0g reduced glutathione (GSH) and 0.0006g N-hydroxysuccinimide (NHS) were dissolved in 10mL deionized water, stirred evenly at 250rpm for 30min, and GSH and NHS The mixed solution was added into the flask; 0.048g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) was dissolved in 10mL of deionized water, after stirring evenly, it was Add in the flask; at room temperature, adjust the reaction mixture in the flask to pH 4.0-5.8 with 0.5mol / mL HCl solution, and react for 6 hours under uniform stirring at a speed of 300rpm; then the product is placed in a dialysis bag (molecular weight cut-off value : 8~12kD), soaked in deionized water for 3 days (changing deionized water twice every day), to eliminate unreacted reagents; finally, the mixture solution was freeze-dried at -80°C to obtain the degree of graftin...

Embodiment 2

[0044]1) Preparation of glutathione-modified carboxymethyl chitosan (CMCS-GSH): Dissolve 0.5 g carboxymethyl chitosan ( CMCS) was made into a homogeneous solution; 0.10 g of reduced glutathione (GSH) and 0.011 g of N-hydroxysuccinimide (NHS) were dissolved in 10 mL of deionized water, stirred evenly at 250 rpm for 30 min, and GSH and The mixed solution of NHS was added in the flask; 0.096g 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) was dissolved in 10mL deionized water, after stirring evenly, the It is added in the flask; at room temperature, adjust the reaction mixture in the flask to pH 4.0-5.8 with 0.6mol / mL HCl solution, and react for 4 hours under uniform stirring at a speed of 300rpm; then the product is placed in a dialysis bag (molecular weight cut-off Value: 8-12kD), soaked in deionized water for 3 days (change the deionized water twice a day) to eliminate unreacted reagents; finally, the mixture solution was freeze-dried at -80°C to obtain the ...

Embodiment 3

[0047] 1) Preparation of glutathione-modified carboxymethyl chitosan (CMCS-GSH): Dissolve 0.5 g carboxymethyl chitosan ( CMCS) to make a homogeneous solution; 0.19g reduced glutathione (GSH) and 0.011g N-hydroxysuccinimide (NHS) were dissolved in 10mL deionized water, stirred evenly at 250rpm for 30min, and GSH and The mixed solution of NHS was added in the flask; 0.096g 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) was dissolved in 10mL deionized water, after stirring evenly, the It is added in the flask; at room temperature, adjust the reaction mixture in the flask to pH 4.0-5.8 with 0.5mol / mL HCl solution, and react for 5 hours under uniform stirring at a speed of 300rpm; then the product is placed in a dialysis bag (molecular weight cutoff Value: 8-12kD), soaked in deionized water for 3 days (change the deionized water twice a day) to eliminate unreacted reagents; finally, the mixture solution was freeze-dried at -80°C to obtain the degree of grafting (...

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Abstract

The invention discloses a copper metal organic framework nanoparticle functionalized hydrogel, a preparation method and application thereof. The preparation method comprises the following steps: dissolving carboxymethyl chitosan, and adding reduced glutathione and N-hydroxysuccinimide which are mixed and dissolved in deionized water; adding 1-(3-dimethylaminopropyl)-3-ethyl carbodiimide hydrochloride to prepare glutathione modified carboxymethyl chitosan; preparing copper metal organic framework nanoparticles; then mixing the copper metal organic framework nanoparticles with deionized water; and adding the glutathione modified carboxymethyl chitosan, acrylamide, polyethylene glycol diacrylate, polyethylene glycol diglycidyl ether and an initiator to prepare the copper metal organic framework functionalized hydrogel. According to the invention, the obtained hydrogel has the capabilities of slowly and controllably releasing copper ions, removing active oxygen of a wound surface, always keeping the wound surface free of hydrops and a wet 3D microenvironment, protecting the wound from external injury and promoting wound healing.

Description

technical field [0001] The invention relates to a functionalized hydrogel, in particular to a copper metal organic framework nanoparticle functionalized hydrogel and its preparation method and application; it belongs to the field of medical devices. Background technique [0002] According to statistics, every year tens of millions of people in China have skin trauma caused by accidents and operations, and hundreds of thousands of people die due to wound infection and failure to heal. Among them, chronic wounds are the most difficult type of wounds to heal. Chronic wound sites produce high concentrations of the by-product H 2 o 2 and other reactive oxygen species (ROS) and pro-inflammatory mediators. Excessive production of ROS will lead to oxidative stress, resulting in impaired function of skin fibroblasts and keratinocytes; a large amount of exudate from chronic wounds is easily infected by pathogenic microorganisms, Cause physiological dysfunction of human tissues. Tr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08J3/24A61L26/00C08L5/08C08L87/00C08L71/02C08B37/08C08F283/06C08F220/56C08G83/00
CPCC08J3/075C08J3/246C08J3/243A61L26/008A61L26/0066A61L26/0023A61L26/0019C08B37/003C08F283/065C08G83/008A61L2300/102A61L2300/404C08J2305/08C08J2487/00C08J2471/02C08J2387/00C08J2405/08C08L5/08C08L71/02C08F220/56
Inventor 彭晓宏马晓峰
Owner SOUTH CHINA UNIV OF TECH
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