Culture media for hepatocyte culture and liver organ preparation

A technology of culture medium and basal medium, applied in cell culture active agent, hepatocyte, culture process, etc., can solve problems such as genome instability

Active Publication Date: 2019-10-11
CENT FOR EXCELLENCE IN MOLECULAR CELL SCI CHINESE ACAD OF SCI
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  • Abstract
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Problems solved by technology

However, recent studies have shown that this in vitro reprogramming may lead to genetic and epigenetic changes, induce genome instability and gene mutations, and the transplantation of genetically modified biological materials into the human body will face great ethical issues. Transplantation of hES- and hiPS-derived "hepatocyte-like" cells poses enormous risks

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  • Culture media for hepatocyte culture and liver organ preparation
  • Culture media for hepatocyte culture and liver organ preparation
  • Culture media for hepatocyte culture and liver organ preparation

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Embodiment Construction

[0078] It should be understood that within the scope of the present invention, the above-mentioned technical features of the present invention and the technical features specifically described in the following (such as embodiments) can be combined with each other to form a preferred technical solution.

[0079] It is well known in the art that mature hepatocytes usually do not survive for more than 1 week under in vitro culture conditions, and cannot proliferate under ordinary in vitro culture conditions. To solve this problem, here is provided a method for culturing liver cells, which includes the step of bringing a cell culture solution containing isolated liver cells into contact with an extracellular matrix (ECM). The inventors found that cultivating hepatocytes by using the method of the present invention can increase the survival time of hepatocytes, maintain the differentiation characteristics of mature hepatocytes, and realize the persistence of differentiated hepatocyt...

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Abstract

The invention provides a proliferation culture medium and differentiation culture medium for hepatocyte culture and liver organ preparation. The proliferation culture medium and the differentiation medium both take a culture medium for growth of mammalian cells as a basic culture medium, and an agent for supplementing L-glutamine, a pH value modifier for maintaining the pH values of the culture media stable, a primary cell culture antibiotic, a serum substitute, N-acetylcysteine, arbitrary nicotinamide and any one or more of a BMP inhibitor, a Wnt agonist, a growth factor, a Rock signaling pathway inhibitor, a P38 signal path inhibitor, a Notch signal path inhibitor, dexamethasone, BMP7 and a cAMP activator are added into the culture media. By using the culture media for culturing liver cells, functional liver organs can be obtained.

Description

technical field [0001] The present invention relates to the cultivation of liver cells and the establishment of organoids, in particular, the present invention relates to a medium for the cultivation of hepatocytes and the preparation of liver organoids. Background technique [0002] The liver is the largest detoxification organ in the human body, the largest digestive gland in the human body, and the center of the body's material and energy metabolism. The liver is mainly composed of two types of epithelial cells, hepatocytes and cholangiocytes. The main functions of the liver include: storing glycogen, synthesizing basic secretory proteins, removing exogenous compounds, regulating body metabolism, transporting drugs, and excreting bile. It is precisely because the liver has important physiological functions that many diseases related to the liver, such as hepatitis, liver cancer, liver fibrosis, liver cirrhosis, acute and chronic liver failure, etc., have high morbidity a...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071
CPCC12N5/0671C12N2500/32C12N2500/30C12N2501/155C12N2501/415C12N2501/10C12N2501/01C12N2501/999C12N5/067
Inventor 高栋刘文明何娟
Owner CENT FOR EXCELLENCE IN MOLECULAR CELL SCI CHINESE ACAD OF SCI
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