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Inoculation method suitable for medical bombyx batryticatus production

A kind of silkworm, suitable technology, applied in the field of animal medicine breeding, can solve the problems of unfavorable standardized production, inconsistent inoculation time, uncontrolled temperature and humidity, etc., and achieve the effect of easy standardized operation, guaranteed morbidity rate and reliable quality

Inactive Publication Date: 2019-01-18
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Currently reported silkworm inoculation methods are mostly based on the experience of technicians, with various inoculation methods, random handling of silkworms, different inoculation times, and uncontrolled temperature and humidity after inoculation, resulting in unsatisfactory inoculation effects of silkworms, uneven dead time of silkworms, and low yield. , the quality is unstable, which is not conducive to standardized production, affecting the income of farmers and the safety of drug use

Method used

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  • Inoculation method suitable for medical bombyx batryticatus production
  • Inoculation method suitable for medical bombyx batryticatus production
  • Inoculation method suitable for medical bombyx batryticatus production

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Feeding method before embodiment 1 dead silkworm inoculation

[0022] 1. Experimental materials

[0023] Bombyx mori Linnaeus 5th instar larva of Bombyx mori insects, Beauveria assiana (Bals.) Vuillant, Tween-80.

[0024] 2. Experimental design

[0025] Prepare the stock solution of Beauveria bassiana with sterile water containing 0.05% Tween 80 (23-28°C), measure the concentration of spores with a hemocytometer, and dilute the stock solution of Beauveria bassiana to 1×10 8 pieces / mm2. Set 4 feeding times, feeding 0 times before inoculation, feeding 1 time, feeding 2 times, feeding 3 times, 3 repetitions in each group, a total of 12 treatment areas. The feeding temperature is 26±2°C, the humidity is 70±5%RH, light / dark (10:14) environment, and the amount of mulberry feeding is based on full satiety.

[0026] 3. Results and analysis

[0027] 3.1 Effect of feeding before inoculation on silkworm growth indicators

[0028] Feeding times increase, the dead time prolong...

Embodiment 2

[0042] Embodiment 2 Bombyx mori inoculation method

[0043] 1. Experimental materials

[0044] Bombyx mori Linnaeus 5th instar larva of Bombyx mori insects, Beauveria assiana (Bals.) Vuillant, Tween-80.

[0045] 2. Experimental design

[0046] 2.1 Screening of inoculation methods

[0047] Healthy fifth-instar silkworm larvae were selected for inoculation. Prepare the stock solution of Beauveria bassiana with sterile water containing 0.05% Tween 80 (23-28°C), measure the spore concentration with a hemocytometer, and dilute the stock solution of Beauveria bassiana to 1×10 8pieces / mm2. Set up a blank group and an experimental group. The blank group is not inoculated, and the experimental group adopts 3 inoculation methods, method 1 (infiltration), method 2 (spray), and method 3 (feeding), with 3 repetitions in each group, a total of 12 treatment areas . After inoculation, the ambient temperature is 24-26°C, and the humidity is 90±5%RH. The amount of mulberries given after 1...

Embodiment 3

[0075] Embodiment 3 Bombyx mori inoculation time selection method

[0076] 1. Experimental materials

[0077] Bombyx mori Linnaeus 5th instar larva of Bombyx mori insects, Beauveria assiana (Bals.) Vuillant, Tween-80.

[0078] 2. Experimental design

[0079] According to the result of Example 1, because the time used for feeding silkworms once is different, it is not easy to control in actual production, so the experiment is carried out without feeding before inoculation.

[0080] 2.1 Preliminary study on inoculation time

[0081] Prepare the stock solution of Beauveria bassiana with sterile water containing 0.05% Tween 80 (23-28°C), measure the concentration of spores with a hemocytometer, and dilute the stock solution of Beauveria bassiana to 1×10 8 pieces / mm2. The working solution of Beauveria bassiana was inoculated at the 0h, 2h, 4h, and 6h of silkworm initiation respectively, and each group had 3 replicates, with a total of 12 treatment areas. The feeding temperatur...

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Abstract

The invention provides an inoculation method suitable for medical bombyx batryticatus production. The inoculation method comprises the following steps of a, early breeding, wherein mulberry leaves areused as food, and bombyx mori linnaeus is artificially raised; regular feeding with the mulberry leaves, wherein the bombyx mori linnaeus is fed with the mulberry leaves 3-4 times every day; screen arrangement for litter cleaning; treatment before sleep; b, inoculation, wherein a beauveria bassiana working solution (1*10<6>-1*10<8> heads / m<2>) is prepared by using sterile water containing 0.05% of Tween 80 at the temperature of 23-28 DEG C, and inoculation is conducted. By means of the inoculation method suitable for medical bombyx batryticatus production, the deadweight of the bombyx batryticatus is high, the living number of the bombyx batryticatus is small, the yield is high, standard operation is facilitated, and the inoculation method is suitable for industrial mass production.

Description

technical field [0001] The invention relates to an inoculation method suitable for the production of medicinal silkworms, and belongs to the field of animal medicinal cultivation. Background technique [0002] Bombyx mori is derived from the dead body of Bombyx mori Linnaeus 4-5 instar larvae infected with Beauveria assiana (Bals.) Vuillant. Phlegm, convulsions, epilepsy, convulsions in children, tetanus and other diseases. The traditional medical silkworm comes from the by-products produced by a small amount of silkworms infected with Beauveria bassiana during the silk cocoon breeding process. However, for silkworm cocoon production, Beauveria bassiana is the main control object, which leads to limited production of dead silkworms, and it is difficult to meet the demand for stiff silkworms in the Chinese herbal medicine market. To meet the needs of silkworms, the implementation of artificial large-scale production is an effective way to solve the source of dead silkworms, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/04
CPCA01K67/04
Inventor 李敏陈辉陈蓉敬勇
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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