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Propagation method of Diglyphus isaea by use of Chromatomyia horticola

A technology of Liriomys grandiflorum and Liriomyza solani, which is applied to the application of Liriomyza pea in the propagation of Liriomyza solani, a new use field of Liriomyza pea, which can solve the problems of low lifespan and small host individuals, etc. Achieve the effect of high space utilization, low cost and easy standardized operation

Active Publication Date: 2012-11-14
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At home and abroad, Liriomyza sativae Blanchard is mainly used as the host insect to breed Liriomyza sativae Blanchard, and good results have been achieved, but there are many limitations at the same time, such as small host individuals, low life expectancy

Method used

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  • Propagation method of Diglyphus isaea by use of Chromatomyia horticola
  • Propagation method of Diglyphus isaea by use of Chromatomyia horticola
  • Propagation method of Diglyphus isaea by use of Chromatomyia horticola

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1, the comparative test of different hosts to multiply Liriomyza pisa

[0050] In this embodiment, Liriomyza pea and Liriomyza americanum were respectively used as hosts to multiply Liriomyza pipae, and under the same conditions, the parasitoids obtained by multiplying two different hosts were used in terms of morphology, lifespan, egg production, The differences in the sex ratio of male and female were analyzed and compared.

[0051] 1. Determination of the developmental duration of two kinds of leaf miners

[0052] The developmental stages of two species of leaf miners (Liriomyza pea and Liriomyza americanum) in different stages were measured at a temperature of 24°C, a relative air humidity of 65%-70% and sufficient light conditions, specifically involving egg-larvae-pupa - Determination of the duration of adult development, and determination of adult lifespan.

[0053] Determination of egg-larva-pupa-adult developmental period: put one unmated female fl...

Embodiment 2

[0096] Embodiment 2, utilize Liriomyza pea to rapidly multiply Liriomyza adena

[0097] 1. Cultivation of seedlings

[0098] 1. Selection of seedling varieties

[0099] Choose kidney beans for seedlings, the variety is British red kidney beans, and choose seeds with positive color, large particles, plump, regular shape, and uniform texture.

[0100] 2. Rapid propagation of seedlings

[0101] Rapid propagation conditions: The best environmental conditions for seedling growth: temperature 27°C±0.5°C, relative air humidity 65%-70%, photoperiod L:D=14h:10h.

[0102] 1) Germination and sowing: In a closed and isolated seedling cultivation room, put vermiculite in a plastic tray (60cm×30cm), about 2 / 3 of the pot volume, and moisten the vermiculite evenly (do not water too much , to prevent the bean from rot), then press the bean seeds into the vermiculite, just submerged in the vermiculite, cultivate for about 5 days, and get the bean sprouts with germinated seeds and stems growi...

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Abstract

The invention discloses a new application of Chromatomyia horticola in propagation of Diglyphus isaea. The new application of Chromatomyia horticola provided by the invention is the application of Chromatomyia horticola in propagation of Diglyphus isaea. The application comprises the following steps: inoculating spawning female Diglyphus isaea on plant seedling infected with larva of Chromatomyia horticola, performing cultivation, and collecting Diglyphus isaea to realize propagation. The application takes Chromatomyia horticola as host; repeatedly researches and improves in technical links of optimal inoculation proportion, key inoculation time, adult Diglyphus isaea collection and storage, etc.; and forms a high-efficiency, economic and simple parasitic wasp propagation technology, to rapidly propagate Diglyphus isaea in large scale, thereby realizing bio-control of pests.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a new application of Liriomyza pisa, in particular to an application of Liriomyza pisa in the expansion and propagation of Liriomyza pisa. Background technique [0002] In addition to harming the lawn, leafminer pests are the most serious damage to rapeseed, Chinese cabbage, potherb mustard, etc. in the family Fabaceae, peas and broad beans in the family Fabaceae, Chrysanthemum chrysanthemum in the Asteraceae family and celery in the Umbelliferae family. Leafminer pests have hidden hazards, strong fecundity, and easy to produce drug resistance. It is difficult to completely control them with conventional chemical control methods, resulting in a vicious circle of increased control costs, reduced effects, and environmental pollution. According to data, the economic loss caused by the harm of leafminer pests in my country is as high as 2 billion yuan every year. Therefore, people are pay...

Claims

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Application Information

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IPC IPC(8): A01K67/033A01G31/00
Inventor 张礼生陈红印顾新丽
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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