Environmental control method suitable for medicinal bombyx batryticatus production

A control method and technology of silkworms, applied in animal husbandry and other fields, can solve problems such as uneven quality of silkworms and thin and small silkworms, and achieve the effects of easy standardized operation, improved yield and quality, and guaranteed success rate

Inactive Publication Date: 2019-07-30
SICHUAN GUANGYUAN MINJIANG CHINESE HERBAL MEDICINE PLANTING CO LTD
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In actual production, there are hollow silkworms and thin silkworms, which lead to uneven quality of silkworms in the market

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Environmental control method suitable for medicinal bombyx batryticatus production
  • Environmental control method suitable for medicinal bombyx batryticatus production
  • Environmental control method suitable for medicinal bombyx batryticatus production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1 Bombyx mori inoculation environment control method

[0025] 1 Experimental materials

[0026] Silkworm Bombyx mori Linnaeus silkworm eggs (purchased from Langzhong Silkworm Farm, Sichuan Province), Beauveria assiana (Bals.) Vuillant (provided by Chengdu University of Traditional Chinese Medicine), Tween-80.

[0027] 2 Experimental design

[0028] Prepare the stock solution of Beauveria bassiana with sterile water containing 0.05% Tween 80 (23-28°C), measure the concentration of spores with a hemocytometer, and dilute the stock solution of Beauveria bassiana to 1×10 6 ~1×10 8 piece / mm 2 .

[0029] Five-instar silkworms with uniform size and good growth conditions were selected and inoculated with Beauveria bassiana. The inoculation environment was controlled, with 3 replicates in each group, and a total of 12 treatment areas. The environmental control is shown in Table 1. The fifth-instar silkworm is cultured at a temperature of 26±2°C, a humidity of 75...

Embodiment 2

[0047] Embodiment 2 Bombyx mori inoculation method

[0048] 1 Experimental materials

[0049] Silkworm Bombyx mori Linnaeus silkworm eggs (purchased from Langzhong Silkworm Farm, Sichuan Province), Beauveria assiana (Bals.) Vuillant (provided by Chengdu University of Traditional Chinese Medicine), Tween-80.

[0050] 2 Experimental design

[0051] 2.1 Screening of inoculation methods

[0052] Healthy fifth-instar silkworm larvae were selected for inoculation. Set up a blank group and an experimental group. The blank group is not inoculated, and the experimental group adopts 3 inoculation methods, method 1 (infiltration), method 2 (spray), and method 3 (feeding), with 3 repetitions in each group, a total of 12 treatment areas , the operation details are shown in Table 1. After inoculation, the ambient temperature is 24-28°C, the humidity is 90±5%RH, and the amount of mulberry is controlled after 2 hours. The fifth-instar silkworm is cultured at a temperature of 26±2°C, a hu...

Embodiment 3

[0076] Embodiment 3 Bombyx mori inoculation time selection method

[0077] 1 Experimental materials

[0078] Silkworm Bombyx mori Linnaeus silkworm eggs (purchased from Langzhong Silkworm Farm, Sichuan Province), Beauveria assiana (Bals.) Vuillant (provided by Chengdu University of Traditional Chinese Medicine), Tween-80.

[0079] 2 Experimental design

[0080] 2.1 Preliminary study on inoculation time

[0081] The fifth-instar silkworm larvae were selected and inoculated with the working solution of Beauveria bassiana at the 0h, 2h, 4h, and 6h of silkworm emergence, with 3 replicates in each group, and a total of 12 treatment areas. Feeding temperature is 26±2°C, humidity is 75±5%RH, light / dark (10:14) environment, and the amount of mulberry feeding is based on full satiety.

[0082] 2.2 Optimization of inoculation time

[0083] The fifth-instar silkworm larvae were selected and inoculated with the working solution of Beauveria bassiana at the 0h, 8h, 16h, 24h, and 32h of...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for controlling temperature and humidity for a culture environment. According to the method, the inoculation environment is controlled at 24-28 DEG C and 85-95% RH, a rearing shelf with the area being not less than 30m<2> is needed to be prepared for five-age silkworms per paper, the culture environment is controlled at 24-28 DEG C and 70-80% RH, debris is removed,and ventilation for air exchange is performed once every 6 hours. The invention also provides a breeding method of high-quality and high-yield medicinal bombyx batryticatus. The breeding method comprises the following steps: a, pre-culture: regularly supplying mulberry leaves, screening and removing sand, and performing pre-sleep treatment; b, inoculation environment: adjusting the temperature andhumidity of an inoculation room, and performing ventilation for air exchange; c, inoculation: performing spray inoculation three times within 0 to 16 hours after silkworm forming; d, spacing: spacingday by day, wherein the area of the rearing shelf is not less than 30m<2>. The method provided by the invention is suitable for artificially large-scale breeding of medicinal bombyx batryticatus, theyield is high and the quality is good.

Description

technical field [0001] The invention relates to an environment control method suitable for the production of medicinal silkworms. Background technique [0002] Bombyx mori is derived from the dry body of Bombyx mori Linnaeus 4-5 instar larvae infected with Beauveria assiana (Bals.) Vuillant, which is a typical animal drug. Fibrinolytic, anti-cancer, lipid-lowering, cosmetic and other pharmacological effects. With the in-depth research on the pharmacological components and functions of Bombyx mori, the application will become more and more extensive. The quality of Bombyx mori can greatly affect its research and development, so it is very important to study the breeding methods of Bombyx mori with high quality and high yield. [0003] Application number: 201710507501.2, Invention name: A method for applying bacteria to large-scale cultured Bombyx mori, which discloses a method for applying bacteria to large-scale cultured Bombyx mori, in which the humidity in the breeding r...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/04
CPCA01K67/04
Inventor 何刚李永川肖禾刘文武
Owner SICHUAN GUANGYUAN MINJIANG CHINESE HERBAL MEDICINE PLANTING CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap