Environmental control method suitable for medicinal bombyx batryticatus production
A control method and technology of silkworms, applied in animal husbandry and other fields, can solve problems such as uneven quality of silkworms and thin and small silkworms, and achieve the effects of easy standardized operation, improved yield and quality, and guaranteed success rate
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Embodiment 1
[0024] Embodiment 1 Bombyx mori inoculation environment control method
[0025] 1 Experimental materials
[0026] Silkworm Bombyx mori Linnaeus silkworm eggs (purchased from Langzhong Silkworm Farm, Sichuan Province), Beauveria assiana (Bals.) Vuillant (provided by Chengdu University of Traditional Chinese Medicine), Tween-80.
[0027] 2 Experimental design
[0028] Prepare the stock solution of Beauveria bassiana with sterile water containing 0.05% Tween 80 (23-28°C), measure the concentration of spores with a hemocytometer, and dilute the stock solution of Beauveria bassiana to 1×10 6 ~1×10 8 piece / mm 2 .
[0029] Five-instar silkworms with uniform size and good growth conditions were selected and inoculated with Beauveria bassiana. The inoculation environment was controlled, with 3 replicates in each group, and a total of 12 treatment areas. The environmental control is shown in Table 1. The fifth-instar silkworm is cultured at a temperature of 26±2°C, a humidity of 75...
Embodiment 2
[0047] Embodiment 2 Bombyx mori inoculation method
[0048] 1 Experimental materials
[0049] Silkworm Bombyx mori Linnaeus silkworm eggs (purchased from Langzhong Silkworm Farm, Sichuan Province), Beauveria assiana (Bals.) Vuillant (provided by Chengdu University of Traditional Chinese Medicine), Tween-80.
[0050] 2 Experimental design
[0051] 2.1 Screening of inoculation methods
[0052] Healthy fifth-instar silkworm larvae were selected for inoculation. Set up a blank group and an experimental group. The blank group is not inoculated, and the experimental group adopts 3 inoculation methods, method 1 (infiltration), method 2 (spray), and method 3 (feeding), with 3 repetitions in each group, a total of 12 treatment areas , the operation details are shown in Table 1. After inoculation, the ambient temperature is 24-28°C, the humidity is 90±5%RH, and the amount of mulberry is controlled after 2 hours. The fifth-instar silkworm is cultured at a temperature of 26±2°C, a hu...
Embodiment 3
[0076] Embodiment 3 Bombyx mori inoculation time selection method
[0077] 1 Experimental materials
[0078] Silkworm Bombyx mori Linnaeus silkworm eggs (purchased from Langzhong Silkworm Farm, Sichuan Province), Beauveria assiana (Bals.) Vuillant (provided by Chengdu University of Traditional Chinese Medicine), Tween-80.
[0079] 2 Experimental design
[0080] 2.1 Preliminary study on inoculation time
[0081] The fifth-instar silkworm larvae were selected and inoculated with the working solution of Beauveria bassiana at the 0h, 2h, 4h, and 6h of silkworm emergence, with 3 replicates in each group, and a total of 12 treatment areas. Feeding temperature is 26±2°C, humidity is 75±5%RH, light / dark (10:14) environment, and the amount of mulberry feeding is based on full satiety.
[0082] 2.2 Optimization of inoculation time
[0083] The fifth-instar silkworm larvae were selected and inoculated with the working solution of Beauveria bassiana at the 0h, 8h, 16h, 24h, and 32h of...
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