HPLC measuring method for three ingredients of trinary mycin for injection

A technique for triple mycin and injection, applied in the field of analysis, can solve the problems of long measurement time and complicated measurement steps, and achieve the effects of strong accuracy, convenient standardized operation and high sensitivity

Inactive Publication Date: 2015-11-18
陈汝红
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Problems solved by technology

At present, the detection methods of benzathine penicillin, procaine penicillin and penicillin sodium in triplemycin for injection are mainly determined separately by HPLC method, which has the defects of cumbersome determination steps and long determination time

Method used

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  • HPLC measuring method for three ingredients of trinary mycin for injection
  • HPLC measuring method for three ingredients of trinary mycin for injection
  • HPLC measuring method for three ingredients of trinary mycin for injection

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Embodiment 1

[0038] A kind of HPLC assay method of three components in triplemycin for injection, it specifically comprises the steps:

[0039] 1) Preparation of reference substance solution Precisely weigh about 12 mg of procaine working reference substance, about 20 mg of dibenzylethylenediamine reference substance and about 80 mg of penicillin working product, put them in a 100ml measuring bottle, add 10ml of acetonitrile and shake to make the mixture evenly After dispersion, add 10ml of methanol and shake well to dissolve, immediately dilute to the mark with phosphate buffer, shake well, and use it as the reference solution.

[0040] 2) Preparation of the test solution Precisely weigh about 50 mg of the test product, put it in a 50 ml measuring bottle, add 10 ml of acetonitrile and shake to make it evenly dispersed, add 10 ml of methanol and shake to dissolve it, and immediately use phosphate buffer (take Potassium dihydrogen phosphate 6.8g and dipotassium hydrogen phosphate 1.14g in 1...

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Abstract

The invention relates to an HPLC measuring method for three ingredients of trinary mycin for injection. The HPLC measuring method comprises the following steps: 1), preparation of a reference solution; 2), preparation of a test solution; 3), chromatographic conditions, wherein the chromatographic column is a C18 reversed-phase chromatographic column, the mobile phase is a buffer salt solution-acetonitrile of which the volume ratio is 81:19, the buffer salt solution contains 0.21 mol/L of monopotassium phosphate and 0.16 percent of triethylamine, the pH value is 5.6, the flow velocity is 1.0 ml/min, the detection wavelength is 215 nm, and the sample size is 10 [mu]l; 4), content calculation, wherein the content calculation method adopts the external standard method, and the contents of procaine, dibenzyl ethylene diamine and penicillin in a test sample are calculated according to peak areas. According to the HPLC measuring method, the contents of the three ingredients of the trinary mycin can be measured at the same time, and the HPLC measuring method has the advantages of high repeatability, sensitivity and accuracy, and facilitates standard operation.

Description

technical field [0001] The invention relates to an analysis method, in particular to an HPLC assay method for three components in triplemycin for injection. Background technique [0002] Triplemycin for injection, also known as fortified benzathine penicillin, is a sterile powder mixed with benzathine penicillin, procaine penicillin, and penicillin sodium according to the international unit ratio of 2:1:1. Benzathine penicillin is the dibenzylethylenediamine salt of penicillin, and procaine penicillin is the procaine salt of penicillin, both of which are long-acting penicillins, and the antibacterial active ingredient is penicillin, which slowly dissociates after intramuscular injection Penicillin prolongs the maintenance time of blood concentration and shows long-acting effect. The combination of the three makes it have the advantages of long-term, medium-term and immediate effect, and can maintain the effective concentration in the blood for 1-4 weeks. There is no domest...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 陈汝红
Owner 陈汝红
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