The invention belongs to the technical field of onion breeding and molecular detection, and particularly relates to an
InDel marker for identifying onion
cytoplasm genotypes based on an HRM technology and application of the
InDel marker. Specifically, the
InDel molecular marker primer provided by the invention can be used for rapidly and efficiently detecting the onion
cytoplasm genotype at high
throughput, the detection speed is remarkably increased through a high-
throughput PCR technology, and the time and labor cost is reduced. Therefore, high-
throughput genotyping screening of onion field breeding materials is facilitated, and the method is particularly suitable for large-scale sample treatment. Meanwhile, an onion material with a known
cytoplasm type is verified, the identification result is completely consistent with the
phenotype, compared with an SCAR marker, the
screening method is simpler and more convenient,
electrophoresis is not needed after the PCR reaction is finished, the type of the onion cytoplasm can be determined only by analyzing a melting curve, and the method is efficient, accurate and low in cost. The technology can realize batch, automatic and standardized operation, and has good practical application value.