The invention discloses an instrument for perpendicularly assembling a chemical mixer and a penicillin bottle. The instrument for perpendicularly assembling the chemical mixer and the penicillin bottle comprises a base, a rotary shaft, a disk I, a disk II, a support, a guide plate, a connecting rod, a push rod and a curved bar, wherein the rotary shaft is vertically arranged on the base, the rotary shaft is fixedly sleeved with the disk I and the disk II, the guide plate is arranged above the disk II, the curved bar is arranged on the top of the support, an outwards protruding section I of the curved bar is sleeved with one end of the connecting rod, the other end of the connecting rod is hinged to one end of the push rod, the push rod penetrates through the guide plate, a plurality of clamping grooves are formed in the disk I in the circumferential direction of the disk I, the outer sides of the clamping grooves are open, round holes which correspond to the clamping grooves in a one-to-one mode are formed in the disk II, and the other end of the push rod is arranged above a rotation track of the round holes. The instrument for perpendicularly assembling the chemical mixer and the penicillin bottle is integrally designed, and is fixed in occupied space and good in stability; due to the fact that synchronous rotation is formed between the two disks, continuous operation of loading, puncture assembly and unloading can be achieved, and handling capacity is flexible and adjustable; due to the fact that puncture assembly between penicillin bottles of different specifications and infusion bags of different specifications can be achieved through switching between the disks of different specifications, and the instrument for perpendicularly assembling the chemical mixer and the penicillin bottle is wide in range of application.