Immunohistochemical quality control product constructed based on animal organs or tissues

A technology of immunohistochemistry and quality control products, which is applied in the field of pathological examination, can solve the problems of non-standard production process, lack of standards, unknown application data and effect, and achieve the effect of standardized operation and easy acquisition

Pending Publication Date: 2021-11-30
吴鸿雁
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] ③Organoids: Cellular components and connective tissue intermediate filament components similar to human organs, but without in vivo environment and body fluid regulation, and the application data and effects are unknown
[0022] 2. Ethical limitations
[0023] 3. The production process is not standardized
The current production method comes from reference documents, and the industry has not yet published production specifications and standards (the production process and effect need to be evaluated); due to the lack of a unified production process, it is difficult to become a real quality control product
[0024] 4. Lack of standards
[0025] 5. Poor implementation
Therefore, when any one of the slices shows a negative staining result, it is still impossible to confirm the accuracy of the result (whether it is true negative)

Method used

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  • Immunohistochemical quality control product constructed based on animal organs or tissues
  • Immunohistochemical quality control product constructed based on animal organs or tissues
  • Immunohistochemical quality control product constructed based on animal organs or tissues

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0136] Example 1 Design of a single type of control materials. Porcine QC and QC sheet paraffin embodiment

[0137] (1) source material and get: Contact meat processing factory, get healthy pigs source of organs.

[0138] (2) Fixation: Organ acquired fresh pig is divided at a low temperature, and then immediately placed in 10% neutral buffered formalin fixed 24h. In order to ensure adequate fixation fixative, 1cm cut fixed to ensure the continued penetration of fixative every organ along the long axis.

[0139] (3) Production QC paraffin blocks: single pig organs, according 2x2x0.2cm 3 After the split, the processing apparatus into the tissue. Automated tissue was dehydrated program (gradient 70% to 100% dehydrated alcohol to 8h, transparent 2h, waxing 2H), and then embedded in paraffin, made into paraffin tissue blocks. Specific steps in front of the program has to add, need to add to the mix

[0140] (4) Quality Control sheets: Using a rotary microtome paraffin, sectioned accord...

Embodiment 2

[0141] 2 universal design / porcine hybrid multiple organ Examples paraffin / "sandwich volume" sheet production method

[0142] (1) drawn segmentation: Take adequate tissue fixation, the different organs of pigs' liver, kidney, pancreas, spleen, lung, intestine, heart, brain "and other organizations, in the case of the board drawn in accordance with 2x0.5x0.2cm 3 Size into cord-like tissue, select pig casing (commercial), porcine afterbirth cut 5x3cm 3 Microscope lens cleaning paper or a "sandwich roll" mode, different organs of the cord-like tissue wrapped into a mixing about 3x1.5x0.5cm 3 size. Wrapping formed similar to the "elongated candy" shape after.

[0143] Note that, in order to avoid deformation of the shape during post-production, the stapler Zhakou ends. When necessary, the central wire knot "elongated candy."

[0144] (2) Production QC wax block: set the wrapped tissue into specialized pathology tissue treatment instrument, automate dried (program ibid., 70%, 80%, 9...

Embodiment 3

[0146] Example 3 Dyeing Effect of Pig Quality Controls

[0147] (1) Sample product: Take the sample in the middle and lower 1 / 3 region with a control slide with a pig tissue organ ingredient. Pigs paids with the same slides in the same slogan to ensure in-slide control.

[0148] (2) Immunohistochemical testing process: immunohistochemistry with industry standard procedures. The main steps are: 1 dewaxing into the water, 23% hydrogen peroxide block, 3 antigen repair, 4 antecate incubation, 5 secondary anti-binding, 6DAB color, 7 lining sealing.

[0149] (3) Quality control sheet evaluation: Before observing the results of the specimen to observe whether the dye of pig tissue organs satisfies signal requirements:, such as the marker of the dyed smooth muscle connective tissue (such as MHC), it can be seen that the obvious pig flat muscle connective tissue is present. Dab tan signals, rather than smoothing muscular connective tissue completely no signal. Controlling the control of an...

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Abstract

The invention relates to the field of pathological examination, in particular to an immunohistochemical quality control product constructed based on animal organs or tissues. In the scheme provided by the invention, the tissues and organs of the pig can effectively replace human organs and tissues to serve as a source of a reference substance in the aspect of partial immunohistochemical detection. The material is convenient to obtain in the market, and commercialized production of quality control products based on tissue morphology and immunohistochemical inspection in the future is facilitated. According to the basic histological manufacturing requirements, such as fixation by using 10% neutral buffer formalin, an automatic tissue dehydration treatment instrument and the like, the manufacturing of the quality control product wax block can be completed. And standardized operation can be realized. The method is simple, convenient and easy to implement without special methods and change of inspection paths.

Description

Technical field [0001] The present invention relates to the field of pathology, in particular, it relates to a construct based on animal organ or tissue immunohistochemistry control materials. Background technique [0002] Clinical immunohistochemistry (immunohistochemistry, immunohistochemistry) may assist in the diagnosis of benign and malignant tumors and to guide treatment. Commercial use of known antibodies to detect tumor antigens. Immunohistochemical clinical pathology irreplaceable as diagnostic tools, which will directly affect the accuracy of the detection result of the pathological diagnosis. [0003] However, due to commercial confidentiality antibody sequences, antibody quality (good and bad mixed) can not achieve unity. Thus the need for clinical immunohistochemistry (anti-human) antibodies using quality control materials confirmation purposes. Meanwhile immunohistochemistry (antigen-antibody reaction) is affected by many factors (including room temperature and humi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30G01N1/36G01N33/53G01N33/531
CPCG01N1/30G01N1/36G01N33/53G01N33/531G01N2001/366
Inventor 吴鸿雁
Owner 吴鸿雁
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