Method for producing fibrous protein-removed original blood from newborn calf blood

Abstract

The invention discloses a method for producing fibrous protein-removed original blood from newborn calf blood. The method comprises the following steps: (1) receiving the blood of a newborn calf by a blood bottle and starting to stir the blood by a glass rod with a bulge at the stirring end at a speed of 110-140 rpm when the blood flows to 1 / 6 to 1 / 4 of the holding volume of the blood bottle; (2) stopping the stirring when the blood achieves the holding volume of the blood bottle and sealing the blood bottle; (3) stirring at a speed of 160-200 rpm for 2-4 min, stopping the stirring, standing for 3-4 min, stirring at 110-140 rpm for 5-8 min, stopping the stirring, and taking out the glass rod and the fibrous protein wound on the stirring end. The method provided by the invention has the advantages that the stability is high and the homogenization degree is high; the automatic stirring is realized so as to avoid the interferences of human factors; the standard operation and industrial production can be realized; the fibrous protein can be removed to a relatively complete extent without filtration, so that the time and the labor are saved; the bottle does not need to change and the blood bottle is a storage bottle for the non-solidified original blood; the recovery rate of the original blood is high and has an average of 93%.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for producing newborn bovine blood defibrinogen blood. Background technique [0002] In the existing production process, there are some methods for removing fibrin in blood, but no large-scale and automated production has been formed. The methods are roughly divided into two types: [0003] The first is to use mechanical stirring to drive the glass beads and blood to mix and rotate, so that the glass beads and blood can fully contact to activate the precipitation of fibrin. The general process is as follows: before blood collection, add a certain amount of glass beads to a clean glass container, The diameter is between 0.5 and 0.7 cm, and then the blood is collected through the carotid artery of the living animal. After a certain amount of blood is inserted into the glass container, the container is manually rotated and mixed upside down to make the blood and the glass beads...

AI Technical Summary

Problems solved by technology

[0005] 1. A large number of glass beads will be used in the mass production process, and the glass beads will produce glass powder and fragments due to mutual friction and collision during long-term use. If the filtration is not complete, the blood quality will be affected.

[0006] 2. This method has not yet been automated, and it mainly relies on manual shaking and vibration to ensure stability and uniformity in the mass production process, and it consumes a lot of labor

[0007] 3. During the artificial shaking and shaking process, if the strength and speed are not properly controlled, the precipitated fibrin can easily embed the glass beads, and finally form a large clot, reducing the yield of the original blood

[0008] 4. Fibrin, glass beads and non-coagulated blood can only be effectively separated by filtration, and the operation is cumbersome

[0011] 1. This method is not suitable for mass or large-scale production

[0012] 2. During the stirring process, the beaker or the triangular flask is not easy to seal or the seal is not tight, which is easy to cause pollution

[0013] 3. Long-term manual stirring is difficult to ensure the uniformity of stirring, and it is prone to incomplete defibrin

[0014] 4. This method is relatively random and there is no unified standard. In the actual operation process, the stability of the results will be directly affected due to the differences in individual operations and techniques.

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