Field planting method for efficiently mating maize diallel crossing combinations
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A technology of double row hybridization and planting method, which is applied in the fields of botanical equipment and methods, plant genetic improvement, application, etc., can solve the problems of pollen hybrid field listing and harvesting, low preparation efficiency, and error-prone, and reduce pollen hybridization. probability, less error-prone, and the effect of reducing errors
Inactive Publication Date: 2019-09-17
CHONGQING ACAD OF AGRI SCI
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[0006] Aiming at the deficiencies of the above-mentioned existing design methods, the object of the present invention is to provide a field planting method for efficiently combining corn dialle hybrid combinations to solve the problems of low preparation efficiency, cumbersome operation, easy pollen mixing and field It is easy to make mistakes in the listing and harvesting process
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Embodiment 1
[0031] In the winter of 2016, a 13×8 incomplete diallel hybrid was prepared in Xishuangbanna, Yunnan ( Figure 4 ).
[0032] 1) Female parent group: 13 maize inbred lines newly selected by Chongqing Academy of Agricultural Sciences (number: 16Y118-16Y130); male parent group: backbone lines Yu 1193, Yu 213, YA8201, LH8012 and 4 Chongqing Academy of Agricultural Sciences Newly selected maize inbred line (number: 16Y114-16Y117).
[0033] 2) Take the respective inbred lines in the male parent group, plant 1 row for each male parent inbred line, a total of 8 rows, with a row length of 7 meters, and plant 16 litters in two phases, the first phase is the same as the female parent inbred line Plant 8 litters, and plant the second period 3 days later.
[0034] 3) Take the respective inbred lines in the female parent group, and plant 1 column for each female parent inbred line, making it 13 female parent columns, and the first row and the first row of male parent selfing of all female p...
Embodiment 2
[0039] In 2017, this method was used to assemble a 16×8 diallel hybrid combination, and the specific experimental steps were the same as in Example 1.
Embodiment 3
[0041] In 2018, this method was used to assemble a 18×8 diallel hybrid combination, and the specific experimental steps were the same as in Example 1.
[0042] In conclusion, using this method for the preparation of diallel hybrid combinations, the advantages of simplicity, time saving, and difficulty in mixing pollen are more obvious in the actual operation.
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Abstract
The invention discloses a field planting method for efficiently mating maize diallel crossing combinations. Male parents are planted in rows, wherein the male parents of the same row are the same male parent selfing line; female parents are planted in lines, wherein the female parents of the same line are the same female parent selfing line, the number of rows of each line of the female parents is equal to that of the types of the male parent selfing lines, the first row of the male parent selfing lines and the first row of all the lines of the female parent selfing lines are planted into one row, the second row of the male parent selfing lines and the second row of all the lines of the female parent selfing lines are planted into one row, and the rest can be done in the same manner until the last row of the male selfing lines and the last row of all the lines of the female parent selfing lines are planted into one row. During pollination, the female parent selfing lines are subjected to castration, the male parent selfing lines pollinate corresponding rows of female parent selfing lines by row in correspondence to the lines, and after the selfing lines are ripe, harvesting is sequentially conducted by row and by line. According to the field planting method, the workload and complicated degree in the preparing links of existing diallel crossing combinations are lowered, the probability of pollen mixing is lowered, the pollination and seed clearing efficiency is improved, errors caused by human negligence are reduced, and the field planting method is suitable for application and popularization of maize breeding within a large range.
Description
technical field [0001] The invention relates to the technical field of hybrid breeding, in particular to a field planting method for efficiently assembling a corn dialle hybrid combination. Background technique [0002] Heterosis is the phenomenon in which a hybrid is superior to its two parents in one or more traits. Utilization of heterosis is the basic idea of maize breeding. Its approach is to breed homozygous parental inbred lines first, and then cross the parental inbred lines to breed strong dominant hybrids. Therefore, maize breeding is "difficult in selecting lines, but focusing on testing and matching". Combining ability refers to the combination ability of various traits between different parents. It is an important index for judging maize inbred lines and a decisive factor related to the use of an inbred line in hybrid combination. [0003] In 1942, Sprague and Taturn proposed the concept of general combining ability and special combining ability. General Co...
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