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Screening method and application of phenol degradation strain

A technology of phenol degradation and screening methods, applied in the field of microorganisms, can solve the problems of high requirements for adsorption materials, narrow application range, large energy consumption, etc., achieve low cost, no secondary pollution, and solve pollution problems

Active Publication Date: 2019-12-31
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The physical method mainly includes the adsorption method, but the adsorption method has high requirements on the adsorption material, which brings about the problem of rising costs, and the regeneration and utilization of the adsorption material is an urgent problem to be solved.
The chemical method mainly includes the oxidation method, but this method consumes a lot of energy, has a narrow scope of application, and is prone to secondary pollution
The removal of phenol from polluted environments by conventional physical and chemical methods is an expensive and environmentally unfriendly process

Method used

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  • Screening method and application of phenol degradation strain
  • Screening method and application of phenol degradation strain
  • Screening method and application of phenol degradation strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Isolation and Identification of Radiation-resistant Acinetobacter APH1

[0040] (1) Take samples

[0041] Soil sample source: Jinneng Science and Technology Factory of Shandong Province.

[0042] (2) Screening and isolation of strains

[0043] The collected soil and mud samples were evenly mixed, 5 grams of the mixture was added to a 250 mL Erlenmeyer flask filled with 50 mL of MSM medium, and phenol was added to make the final concentration 500 mg / L as the sole carbon source. Place the Erlenmeyer flask at 30° C. and culture it on a shaker at 200 rpm for 5 days. Transfer to 50mL fresh MSM medium according to 5% inoculum size, add phenol to make the final concentration 500mg / L, transfer every 5 days, repeat three times, dilute and spread the last culture solution before adding 500mg / L On the solid medium of inorganic salts of phenol, cultivate at 30°C until a single colony grows. Pick a single colony into a fresh inorganic salt liquid medium, select the fas...

Embodiment 2

[0047] Example 2 Growth of Acinetobacter radioresistant APH1 under different culture conditions and optimization of its ability to degrade phenol

[0048] 1. The optimum temperature for the growth and degradation of the strain: inoculate the strain into 50 mL of MSM medium containing 500 mg / L phenol concentration at pH = 7.0, and place the strain at 20°C, 25°C, 30°C, 37°C, and 42°C respectively. Cultivate on a shaking table with a rotation speed of 200 rpm, measure the OD with a spectrophotometer at regular intervals 600 To detect the cell density, the phenol concentration was detected by high performance liquid chromatography (HPLC). Such as image 3 As shown in a and 3b, the most suitable culture temperature for strain growth and phenol degradation is 30 °C.

[0049] 2. Optimal pH for strain growth and degradation: Inoculate the strain into 50 mL of MSM medium containing 500 mg / L phenol concentration, adjust the pH to 5.0, 6.0, 7.0, and 8.0 with hydrochloric acid and NaOH,...

Embodiment 3

[0051] Example 3 Verification of the maximum tolerance to phenol of Acinetobacter radiation-resistant APH1

[0052] The strain was inoculated into 50 mL of MSM medium with different phenol concentrations of 500, 700, 800, 950, 1150, and 1300 mg / L at an optimum pH of 6.0, and cultured on a shaker at an optimum temperature of 30°C and a speed of 200 rpm. Measure OD with a spectrophotometer at regular intervals 600 To detect the cell density, the phenol concentration was detected by high performance liquid chromatography (HPLC). Such as Image 6 As shown in a and 6b, as the concentration increases, the growth and degradation of the strain become worse. When the phenol concentration is above 1150 mg / L, the cells hardly grow and degrade. This may be because the higher the phenol concentration, the greater the toxicity to the cells. The strain APH1 Can tolerate 950mg / L of phenol.

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Abstract

The invention discloses a screening method and application of a phenol degradation strain, and relates to the technical field of microorganisms. Phenol is used as the only carbon source of the strain,the strain is an acinetobacter radioresistens APH1 (Acinetobacter radioresistens APH1), and is preserved in China Center for Type Culture Collection, Wuhan university, China, the preservation numberis CCTCC M 2019462, and the preservation time is 18 June, 2019. The strain provided by the invention has efficient degradation rate and degradation speed on the phenol, can realize biologic repair ofpolluted soil, can bear various antibiotics, and provides a support basis for repairing pollution in actual environment. The strain is extremely high in rate of removing the phenol in water bodies andsoil, has the advantages of being low in cost, free from secondary pollution, capable of saving energy resources and the like, and has great application prospects.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a screening method and application of a phenol-degrading bacterial strain. Background technique [0002] Phenol, also known as carbolic acid, is an important chemical raw material, widely used in papermaking, oil refining, dyes, textiles, leather manufacturing, synthetic resins, synthetic fibers, medicines and fungicides, etc. , fiber factories and other industrial sewage discharged from the more content of substances. With the rapid development of my country's chemical industry, the amount of industrial wastewater has greatly increased, especially when these improperly treated sewage seeps into the soil, phenol is easily adsorbed on the bottom mud, making its removal more difficult. [0003] my country is a big country that produces and uses phenol, and the market demand for phenol continues to rise. my country's phenol consumption has risen from 450,000 tons in 2001 to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12Q1/689C12Q1/04B09C1/10C02F3/34C12R1/01
CPCC12N1/20C12Q1/689B09C1/10C02F3/34C12R2001/01C12N1/205
Inventor 唐鸿志刘一帆柳宁陶飞许平
Owner SHANGHAI JIAO TONG UNIV
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