Bacillus foecalis alkaligenes and method for catalysis degradation of phynol wastewater

A technology of Alcaligenes faecalis and catalytic degradation, which is applied in the directions of microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problem of unsatisfactory treatment effect of phenol-containing wastewater, low microbial concentration and large floor space and other problems, to achieve the effect of good catalytic degradation effect, high microbial concentration and not easy to run off

A technology of Alcaligenes faecalis and catalytic degradation, which is applied in the directions of microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problem of unsatisfactory treatment effect of phenol-containing wastewater, low microbial concentration and large floor space and other problems, to achieve the effect of good catalytic degradation effect, high microbial concentration and not easy to run off

CN101245328AInactive Publication Date: 2008-08-20XIANGTAN UNIV
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The Alcaligenes faecalis JH 1013 strain was cultured in the beef extract peptone culture solution for 24 hours, the shaking speed was 100 rpm, the temperature was 35°C, and the pH was 7. centrifuge, take the centrifuge and dissolve in phosphate buffer solution with pH 8.0 to obtain a bacterial suspension containing Alcaligenes faecalis, and absorb the Alcaligenes faecalis with a calcium carbonate carrier to obtain immobilized Alcaligenes faecalis. Add the immobilized Alcaligenes faecalis into the phenol wastewater, and the phenol concentration in the phenol wastewater is 1000mg·L -1 , the amount of immobilized Alcaligenes faecalis is 0.5wt% of the weight of phenol wastewater, the shaking rate is 200rpm, the temperature is 32°C, the pH is 8, the time is 18h, and the degradation rate is 99.4%.

Embodiment 2

[0018] The Alcaligenes faecalis JH 1013 strain was cultured in the beef extract peptone culture solution for 24 hours, the shaking speed was 150 rpm, the temperature was 30°C, and the pH was 8. After centrifugal separation, the centrifuge was taken and dissolved in phosphate buffer solution with pH 7.2 to obtain a solution containing Alcaligenes faecalis, and the Alcaligenes faecalis was adsorbed by Mg-Al hydrotalcite carrier to obtain immobilized Alcaligenes faecalis. Add the immobilized Alcaligenes faecalis into the phenol wastewater, and the phenol concentration in the phenol wastewater is 1800mg·L -1 , the amount of immobilized Alcaligenes faecalis is 1.5wt% of the weight of phenol wastewater, the shaking speed is 300rpm, the temperature is 28°C, the pH is 7, the time is 36h, and the degradation rate is 82.9%.

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Abstract

The invention discloses a microbial strain which can effectively degrade phenol and relates to alcaligenes faecalis JH1013 CCTCC NO: M 208006 and a method for degrading phenol wastewater by using a fixed alcaligenes faecalis that is produced by alcaligenes faecalis. The method has the following advantages that: 1. the alcaligenes faecalis is easy to culture, the fixing technique is simple, the catalytic degradation effect is good, the high-concentration phenol can be resistance, the phenol degradation capacity is strong, which can high efficiently degrade the phenol wastewater with the concentration of as high as 2000mg. L<-1> and be used repeatedly; 2. hydrotalcite, hydrotalcite-like compounds, nano calcium carbonate, Gamma-Al2O3 or the loading solid base are used as carriers for preparing the fixed alcaligenes faecalis, the elasticity is good, the temperature and pH value resistances are strong, the mechanical strength is high, the degradation performance is stable and the half-time is long; 3. the carriers of the hydrotalcite, the hydrotalcite-like compounds, the nano calcium carbonate and the Gamma-Al2O3 of the fixed cell can be used repeatedly by calcinations; 4. the fixed alcaligenes faecalis is used for degrading the phenol, the degradation process is simple, the conditions are moderate, the environment is friendly, the bacterial cells are difficult to loss, the concentration of the microorganisms in the carriers is high, the degradation speed is fast, the degradation efficiency is high and the secondary pollution is not easy to be caused.

Description

technical field [0001] The invention relates to a method for catalytically degrading phenol wastewater by using microorganisms Background technique [0002] Phenol is one of the main pollutants in petrochemical, papermaking, coking and textile industrial wastewater, and many countries have listed it as a key pollutant for control. Biological degradation of phenol wastewater has the advantages of simple process, mild conditions, environmental friendliness, low treatment cost, and less secondary pollution. Generally believed to contain phenol 5-500mg·L -1 The wastewater is suitable for biological treatment, but the treatment effect for higher phenol-containing wastewater is not ideal [Zhang Jie et al., Sichuan Environment. 2006, 25(1): 8-10; Sun Min et al., Chinese Journal of Medical Engineering .2004, 12(3):93-94]. Free microorganisms can also degrade high-concentration phenol wastewater (1000-2000mg L -1 )[Gong Bin et al., Journal of Environmental Science. 2006, 37(6): 2...

Claims

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Application Information

Patent Timeline
20 Aug 2008
Publication
CN101245328A
IPC
C12N1/20; C02F3/34; C12R1/05; C02F101/34
Inventors
曾虹燕; 姜和