Aquifer amendment containing multiple active microorganisms and method of preparing the same
The technology of a water body improver and active microorganism is applied in the field of water body improver containing a variety of active microorganisms and its preparation field, which can solve the problems of insufficient adaptability of active microorganisms to the environment, poor storage stability and poor survivability of live bacteria, and the like. Well-stabilized, adaptable and viable, well-tolerated effects
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Embodiment 1
[0020] A water body improver containing multiple active microorganisms and a preparation method thereof. A strain of Bacillus subtilis and a strain of Bacillus licheniformis with excellent performance are obtained through screening;
[0021] First, carry out the fermentation culture of the strain: the microbial strain adopts the method of liquid submerged fermentation culture to carry out single-strain fermentation culture to obtain the fermentation liquid; the culture of the bacteria has no special requirements for the nutrient source in the medium, and can be used commonly used in microbial culture. carbon source, nitrogen source, inorganic salt and other growth factors; the carbon source can be glucose, sucrose, corn flour, etc., the nitrogen source can be peptone, beef extract, yeast extract, soybean meal, etc., and the inorganic salt can be dihydrogen phosphate Potassium, dipotassium hydrogen phosphate, magnesium sulfate heptahydrate, etc., growth factors can be vitamin B ...
Embodiment 2
[0029] A method for preparing a water body improver containing multiple active microorganisms. Microbial strains with excellent performance are obtained through screening: a strain of Bacillus subtilis and a strain of Bacillus licheniformis are fermented and cultured by a single strain to obtain a fermentation liquid; Bacillus subtilis The total number of viable bacteria in the fermentation broth 10 6 ~10 10 pcs / ml, the total number of live bacteria in Bacillus licheniformis fermentation broth 10 6 ~10 10 Individual / ml; then mix 50% of Bacillus subtilis fermented bacterium liquid and 50% of Bacillus licheniformis fermented bacterium liquid by volume percentage to obtain mixed bacterium liquid; Add auxiliary agent sodium benzoate and yellow pigment, after mixing evenly, obtain liquid preparation, this liquid preparation contains the total number of viable bacteria to be 10 6 ~10 10 per milliliter, and the composition of the bacterial population ratio is relatively stable. ...
Embodiment 3
[0035] A method for preparing a water body improver containing multiple active microorganisms. Microbial strains with excellent performance are obtained through screening: a strain of Bacillus subtilis and a strain of Bacillus licheniformis are fermented and cultured by a single strain to obtain a fermentation liquid; Bacillus subtilis The total number of viable bacteria in the fermentation broth 10 6 ~10 10 pcs / ml, the total number of live bacteria in Bacillus licheniformis fermentation broth 10 6 ~10 10 Individual / ml; Mix 50% of Bacillus subtilis fermented bacterium liquid and 50% of Bacillus licheniformis fermented bacterium liquid by volume percentage again, obtain mixed bacterium liquid; Then by mass-volume ratio rice bran powder: mixed bacterium liquid is 2: 1 mixes Adsorption and flash drying to obtain solid bacterial powder; then add auxiliary material zeolite powder to the solid bacterial powder, and after mixing evenly, obtain a solid preparation, which contains a ...
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