Aquifer amendment containing multiple active microorganisms and method of preparing the same

The technology of a water body improver and active microorganism is applied in the field of water body improver containing a variety of active microorganisms and its preparation field, which can solve the problems of insufficient adaptability of active microorganisms to the environment, poor storage stability and poor survivability of live bacteria, and the like. Well-stabilized, adaptable and viable, well-tolerated effects

Inactive Publication Date: 2008-01-23
SHANGHAI SIJI BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 1) The active microorganisms in the product have insufficient ability to adapt to the environment after entering the natural water body, and their survival ability in the natural water body is poor. Successful microbial water body improvement products must test and screen the microbial strains that make up the preparation, and are currently widely used in the market Most of the products have not carried out targeted strain screening research on the microorganisms that make up the products and their adaptability to natural water bodies;
[0005] 2) Poor storage stability of live bacteria at room temperature is a common problem in this type of product, how to maintain the stability of live bacteria at room temperature is an urgent technical problem to be solved
[0006] 3) The function of the product is single and the effect is unstable, so it is not suitable for various water bodies

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A water body improver containing multiple active microorganisms and a preparation method thereof. A strain of Bacillus subtilis and a strain of Bacillus licheniformis with excellent performance are obtained through screening;

[0021] First, carry out the fermentation culture of the strain: the microbial strain adopts the method of liquid submerged fermentation culture to carry out single-strain fermentation culture to obtain the fermentation liquid; the culture of the bacteria has no special requirements for the nutrient source in the medium, and can be used commonly used in microbial culture. carbon source, nitrogen source, inorganic salt and other growth factors; the carbon source can be glucose, sucrose, corn flour, etc., the nitrogen source can be peptone, beef extract, yeast extract, soybean meal, etc., and the inorganic salt can be dihydrogen phosphate Potassium, dipotassium hydrogen phosphate, magnesium sulfate heptahydrate, etc., growth factors can be vitamin B ...

Embodiment 2

[0029] A method for preparing a water body improver containing multiple active microorganisms. Microbial strains with excellent performance are obtained through screening: a strain of Bacillus subtilis and a strain of Bacillus licheniformis are fermented and cultured by a single strain to obtain a fermentation liquid; Bacillus subtilis The total number of viable bacteria in the fermentation broth 10 6 ~10 10 pcs / ml, the total number of live bacteria in Bacillus licheniformis fermentation broth 10 6 ~10 10 Individual / ml; then mix 50% of Bacillus subtilis fermented bacterium liquid and 50% of Bacillus licheniformis fermented bacterium liquid by volume percentage to obtain mixed bacterium liquid; Add auxiliary agent sodium benzoate and yellow pigment, after mixing evenly, obtain liquid preparation, this liquid preparation contains the total number of viable bacteria to be 10 6 ~10 10 per milliliter, and the composition of the bacterial population ratio is relatively stable. ...

Embodiment 3

[0035] A method for preparing a water body improver containing multiple active microorganisms. Microbial strains with excellent performance are obtained through screening: a strain of Bacillus subtilis and a strain of Bacillus licheniformis are fermented and cultured by a single strain to obtain a fermentation liquid; Bacillus subtilis The total number of viable bacteria in the fermentation broth 10 6 ~10 10 pcs / ml, the total number of live bacteria in Bacillus licheniformis fermentation broth 10 6 ~10 10 Individual / ml; Mix 50% of Bacillus subtilis fermented bacterium liquid and 50% of Bacillus licheniformis fermented bacterium liquid by volume percentage again, obtain mixed bacterium liquid; Then by mass-volume ratio rice bran powder: mixed bacterium liquid is 2: 1 mixes Adsorption and flash drying to obtain solid bacterial powder; then add auxiliary material zeolite powder to the solid bacterial powder, and after mixing evenly, obtain a solid preparation, which contains a ...

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PUM

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Abstract

The invention relates to a water improver with multiple active microbes and a preparation method thereof, which adopts two or a plurality of following microbial strains, comprising a bacillus licheniformis, a bacillus subtilis, a bacillus megaterium, a lactobacillus plantarum, a saccharomyces cerevisiae, a candida lipolytica, bacillus pumilus, bacillus cereus, a rhodopsedomnas palustris, an alcaligenes faecalis and an arthrobacter globiformis. After the femination of the microbial strains, the preparation of the liquid is that the single microzyme zymocyte liquid is mixed and stirred in proportion; or the mixed bacteria solution is concentrated; or the concentrated single microzyme zymocyte liquid is mixed and stirred according to the proportion of the single microzyme zymocyte liquid, and is prepared into a mix bacteria solution. The mix bacteria solution is mixed homogeniously with an added assistant agent. The total amount of the live bacteria within the warrenty period is 106 to 1010 per milliliter. The preparation of the solid inoculant is that the zymocyte liquid is dried after the absoption of the adsorbent on the zymocyte liquid, is prepared into a solid bacterial powder and mixed evenly with the added assistant agent. The total amount of the live bacteria within the warreny period ranges between 106 and 1011 per gram. The invention has multi-function and stable effect and is suitable for treating various water bodies.

Description

technical field [0001] The invention relates to a water body improver composition containing a variety of active microorganisms and suitable for treating various water bodies such as aquaculture water body, landscape water body and river (lake) channel and a preparation method thereof. Background technique [0002] Chinese Patent Application No. 02145249.0 "Microbial Aquaculture Water Improver and Preparation Method" discloses a microbial aquaculture water improver, which is composed of composite bacteria, microalgae, culture medium, molasses, trace element solution, corn flour, and solidified materials; The method of compound culture of strains enables all strains to be cultivated in the same culture medium; the composite solidification method of compound strains and microalgae is adopted; Its manufacturing process" discloses a living bacteria microbial water-improving agent equipped with an activated medium and its manufacturing method, which is used for water quality regu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C02F3/34C12R1/10C12R1/125C12R1/11C12R1/25C12R1/865C12R1/73C12R1/08C12R1/085C12R1/38C12R1/05C12R1/06
Inventor 宋士良傅利剑黄循一顾宗濂
Owner SHANGHAI SIJI BIOLOGICAL TECH
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