Screening method of alcaligenes faecalis Y311 and application
A technology of Alcaligenes faecalis and strains, applied in the field of Alcaligenes faecalis and its screening, which can solve the problems of few biological species, environmental pollution, weak self-regulation ability, etc.
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Embodiment 1
[0052] The screening of embodiment 1 bacterial strain
[0053] 1 Enrichment and separation and purification of strains
[0054] 1) Enrichment of Alcaligenes faecalis
[0055] (1) Take 4.5g of tilapia culture pond bottom sludge in 100mL sterilized enrichment medium, place it in a shaking table at 27°C, and cultivate it at 140rpm / min for 5 days;
[0056] (2) Take 10ml of the supernatant in (1) and add it into 100mL sterilized enrichment medium, and culture under the same conditions for 5 days;
[0057] (3) Take 10ml of the supernatant in (2) and add it into 100mL sterilized enrichment medium, and culture under the same conditions for 5 days.
[0058] 2) Isolation and purification of Alcaligenes faecalis
[0059] (1) Take 100ul of the supernatant in step (3) during the enrichment process, and serially dilute from 10 times to 10 6 times, respectively spread onto the separation medium, and incubate in a biochemical incubator at a constant temperature of 30°C for 2 days;
[006...
Embodiment 2
[0084] Take 1ml 1.02×10 10 CFU / ml of Y311 physiological saline suspension, inoculated in 180ml rich medium [acetamide (2.0g), NaOH (0.6g), MgSO 4 ·7H 2 O (0.05g), K 2 HPO 4 (8.2g), KCl (0.5g), CuSO 4 ·5H 2 O (0.5mg), CaSO 4 2H 2 O (0.5mg), ZnSO 4 ·7H 2 O (0.5mg), FeCl 3 (0.5mg), distilled water 1000ml, pH7.0], the blank control was added an equal volume of physiological saline (Control). After 7 days, the contents of ammonia nitrogen, nitrate nitrogen and COD in each group were measured. Among them, NH 4 + -N content was determined by Nessler's reagent method; NO 3 - -N content was determined by the phenol disulfonic acid method; COD was determined by the potassium dichromate method.
[0085] After 7d of treatment, Y311 to NH 4 + -N removal rate reached 60.3%, for NO 3 - -N removal rate reached 74.7%, to COD reached 36.7%, the results are shown in Table 1 and Figures 5 to 7 shown.
[0086] Table 1. Alcaligenes faecalis Y311 on NH 4+ -N, NO 3 - Effect o...
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