Screening method of alcaligenes faecalis Y311 and application

A technology of Alcaligenes faecalis and strains, applied in the field of Alcaligenes faecalis and its screening, which can solve the problems of few biological species, environmental pollution, weak self-regulation ability, etc.

Inactive Publication Date: 2018-10-19
PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The aquaculture pond is an autotrophic ecosystem with high organic load, which has few biological species, simple food web, weak self-regulation ability and poor stability
Breeding animals and substrate deposition are the main ways of nitrogen and phosphorus output. During the cultivation of Litopenaeus vannamei, 14% to 53% of the nitrogen in the total input nutrition is deposited in the sediment, and 14% to 28% exists in the culture water, resulting in Huge waste of resources and environmental pollution

Method used

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  • Screening method of alcaligenes faecalis Y311 and application
  • Screening method of alcaligenes faecalis Y311 and application
  • Screening method of alcaligenes faecalis Y311 and application

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Experimental program
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Effect test

Embodiment 1

[0052] The screening of embodiment 1 bacterial strain

[0053] 1 Enrichment and separation and purification of strains

[0054] 1) Enrichment of Alcaligenes faecalis

[0055] (1) Take 4.5g of tilapia culture pond bottom sludge in 100mL sterilized enrichment medium, place it in a shaking table at 27°C, and cultivate it at 140rpm / min for 5 days;

[0056] (2) Take 10ml of the supernatant in (1) and add it into 100mL sterilized enrichment medium, and culture under the same conditions for 5 days;

[0057] (3) Take 10ml of the supernatant in (2) and add it into 100mL sterilized enrichment medium, and culture under the same conditions for 5 days.

[0058] 2) Isolation and purification of Alcaligenes faecalis

[0059] (1) Take 100ul of the supernatant in step (3) during the enrichment process, and serially dilute from 10 times to 10 6 times, respectively spread onto the separation medium, and incubate in a biochemical incubator at a constant temperature of 30°C for 2 days;

[006...

Embodiment 2

[0084] Take 1ml 1.02×10 10 CFU / ml of Y311 physiological saline suspension, inoculated in 180ml rich medium [acetamide (2.0g), NaOH (0.6g), MgSO 4 ·7H 2 O (0.05g), K 2 HPO 4 (8.2g), KCl (0.5g), CuSO 4 ·5H 2 O (0.5mg), CaSO 4 2H 2 O (0.5mg), ZnSO 4 ·7H 2 O (0.5mg), FeCl 3 (0.5mg), distilled water 1000ml, pH7.0], the blank control was added an equal volume of physiological saline (Control). After 7 days, the contents of ammonia nitrogen, nitrate nitrogen and COD in each group were measured. Among them, NH 4 + -N content was determined by Nessler's reagent method; NO 3 - -N content was determined by the phenol disulfonic acid method; COD was determined by the potassium dichromate method.

[0085] After 7d of treatment, Y311 to NH 4 + -N removal rate reached 60.3%, for NO 3 - -N removal rate reached 74.7%, to COD reached 36.7%, the results are shown in Table 1 and Figures 5 to 7 shown.

[0086] Table 1. Alcaligenes faecalis Y311 on NH 4+ -N, NO 3 - Effect o...

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Abstract

The invention provides an alcaligenes faecalis Y311, and a screening method and application thereof. The strain is separated from tilapia culture water environment sediment, and has the preservation number of GDMCC No. 60350. According to the alcaligenes faecalis Y311, the removal rate of ammonia nitrogen in culture wastewater reaches 60.3% within 7 days, the removal rate of nitrate nitrogen reaches 74.7%, and the removal rate of COD reaches 36.7%. Under the aerobic condition, environmental probiotics of ammonia nitrogen, nitrate nitrogen and COD are efficiently degraded, and the alcaligenes faecalis Y311 has a higher application value.

Description

technical field [0001] The invention relates to the technical field of aquatic microorganism application, in particular to Alcaligenes faecalis and its screening method and application. Background technique [0002] Excessive nitrogen has a certain toxic effect on aquatic animals. Therefore, the effective removal of nitrogen in aquaculture water is one of the problems to be solved urgently in the aquaculture industry. Biological denitrification of aquaculture wastewater is a focus of attention and research in the field of water treatment. [0003] The aquaculture pond is an autotrophic ecosystem with high organic load, which has few biological species, simple food web, weak self-regulation ability and poor stability. Breeding animals and substrate deposition are the main ways of nitrogen and phosphorus output. During the cultivation of Litopenaeus vannamei, 14% to 53% of the nitrogen in the total input nutrition is deposited in the sediment, and 14% to 28% exists in the cul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/05C02F103/20
CPCC02F3/34C02F2103/20C12N1/20C12N1/205C12R2001/05
Inventor 衣萌萌王淼卢迈新张德锋刘志刚可小丽高风英曹建萌李忠徽
Owner PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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