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Dry powder biological demulsifying agent and Bacillus alcaligenes applied to petroleum extraction

A biological demulsifier, Alcaligenes technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effects of volume reduction, ease of sale, and wide application range

Inactive Publication Date: 2011-01-19
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The application of chemical demulsifiers has the following problems: ① chemical demulsifiers mainly rely on their surface activity to demulsify, and have strong selectivity to different reservoir types and oil production methods, etc., and poor applicability; ② chemical demulsifiers are generally insoluble Macromolecular organic matter requires organic solvents as additives when used, and long-term exposure will have a certain impact on the health of workers; ③Chemical demulsifiers are refractory substances, once added to the emulsion, it is difficult to remove them from the system After the liquid discharge has become the development trend of produced water treatment, the addition of chemical demulsifiers increases the difficulty of the subsequent treatment process and increases the treatment cost
Although the use of the whole culture solution as a demulsifier in laboratory research can save the extraction process of bacteria or products, there are many problems when the whole culture solution is actually applied to the field, such as other substances in the whole culture solution, especially Hydrocarbons tend to remain in the separated oil phase and water phase, affecting the quality of oil and the effect of water treatment
At the same time, the storage and deterioration of the whole culture medium are also two major problems affecting its field application.

Method used

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  • Dry powder biological demulsifying agent and Bacillus alcaligenes applied to petroleum extraction
  • Dry powder biological demulsifying agent and Bacillus alcaligenes applied to petroleum extraction
  • Dry powder biological demulsifying agent and Bacillus alcaligenes applied to petroleum extraction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Alcaligenes screening:

[0023] Take 3g of oil-contaminated Karamay Oilfield soil and add it to 125mL of the following medium (L -1 ): NaNO 3 8.0g, K 2 HPO 4 2.0g, KH 2 PO 4 3.0g, MgSO 4 ·7H 2 O 0.2g, crude oil 4% (V / V), CaCl 2 2H 2 O 1.0g, MnSO 4 1.0 g, EDTA 1.4 g, pH=7.0. Cultivate in a shaker with a temperature of 30-35°C and a rotation speed of 130-150rpm, with a cycle of 5-7 days, and after 3 cycles, a strain that can be used for petroleum is successfully isolated from it by dilution coating method. Emulsion-breaking Alcaligenes (see figure 1 , the magnification of the sample in the figure is 10,000).

Embodiment 2

[0025] Preparation and use of dry powder biodemulsifier:

[0026] The whole culture solution of Alcaligenes was centrifuged at a high speed of 10000rpm to obtain a wet bacterial culture mixture, which was baked at 105°C for 24 hours and ground to obtain a dry powder (see figure 2 ). The dry powder biological demulsifier can be used at a temperature of 35-75°C, the dosage is 100-200mg / L, shake vigorously for 200 times, and stand for 2.5 hours to observe the demulsification effect.

Embodiment 3

[0028] Changes of surface tension and dehydration rate of Alcaligenes whole culture solution with culture time:

[0029] Use a surface tensiometer to measure the surface tension of the Alcaligenes full culture solution for different culture times, and add the full culture solution to the emulsion at a dosage of 10% (V / V), shake it vigorously for 200 times, and heat it at 35°C. Let it stand for 2.5 hours, observe its demulsification effect, the results are shown in image 3 .

[0030] Depend on image 3 It can be seen that the surface tension of the whole culture solution of Alcaligenes dropped from the initial 56.3mN / m to the lowest value of 29.2mN / m on the 6th day during the cultivation process, which shows that it has generated a certain biological surface activity during the cultivation process. agent. The demulsification effect also changed with the culture time. At the beginning of inoculation, the whole culture solution had almost no demulsification ability, and then...

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Abstract

The invention relates to a dry biological demulsifier powder and an alcaligenes sp. that are used for demulsification in oil exploiting industries. The alcaligenes sp. in oil-polluted oilfield soil is selected, a whole liquid culture medium of the alcaligenes sp. is centrifugalized at the speed of 10000rpm, and a wet mycelium is obtained, baked at the temperature of 105 DEG C for 24 hours and grinded, thus obtaining the dry biological demulsifier powder. From the field tests in oilfield spots, the dry biological demulsifier powder can achieve the dehydration rate of 66.7 to 96.5 percent in 120 to 150 minutes when the addition amount is 100 to 200 milligrams per liter, thus overmatching chemical demulsifiers that are used on the spot. The dry biological demulsifier powder is produced by parching the strain culture medium with demulsification effect, thus solving the inconvenience of the biological demulsifiers with a whole liquid culture medium in the aspects of sale, transportation, storage and the like, and lowering the possibilities that the metamorphic whole liquid culture medium causes the reduction of demulsification effect and other components in the whole liquid culture medium affect oil products and subsequent water processing effect.

Description

technical field [0001] The invention relates to a demulsifier which can be used for oil and water separation, in particular to a method for screening and preparing a dry powder biological demulsifier. Background technique [0002] The demulsification of crude oil emulsion is an important link affecting oil product and oil quantity. At present, the crude oil dehydration agents used in most oilfields in my country are chemical demulsifiers. The chemical demulsifiers mainly include block copolymers or random copolymers of polyoxyethylene and polyoxypropylene, and their relative molecular weights can range from thousands to millions. The application of chemical demulsifiers has the following problems: ① chemical demulsifiers mainly rely on their surface activity to demulsify, and have strong selectivity to different reservoir types and oil production methods, etc., and poor applicability; ② chemical demulsifiers are generally insoluble Macromolecular organic matter requires or...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/05
Inventor 黄翔峰闻岳陆丽君刘佳周琪徐竞成费晓明关炜
Owner TONGJI UNIV
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