Bacterium agent for renovation of organic pollution aquifer, producing and using method of the same

A technology of organic pollution and bacterial agent, applied in the field of microorganisms, can solve the problems of low cost and less nitrobenzene polluted water, and achieve the effects of low cost, continuous strengthening of water purification, and a good society.

A technology of organic pollution and bacterial agent, applied in the field of microorganisms, can solve the problems of low cost and less nitrobenzene polluted water, and achieve the effects of low cost, continuous strengthening of water purification, and a good society.

CN101153271AInactive Publication Date: 2008-04-02SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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Experimental program
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Effect test

Embodiment 1

[0020] The bacterial agent for repairing organically polluted water bodies is composed of facultative anaerobic microorganisms, and the facultative anaerobic microorganisms can be: Pseudomonas stutzeri, Alealigenesfaecalis, Bacillus megaterium ), Enterobacter cloacae (the above strains can be purchased from the market), the ratio of parts by weight is 1:1.5:2.5:5, and the balance is the culture solution containing nitrobenzene.

[0021] The preparation method of bacterial agent: the facultative anaerobic microorganism of 10% by weight is inoculated in the anaerobic bottle that 400ml sterilized culture solution is housed, and the initial concentration of nitrobenzene reaches 30mg / L in the culture solution, seals, and places in 30 Cultivate in an incubator at ℃ for 5 days, then take out 1 / 3 of the culture solution, add the same amount of culture solution and add nitrobenzene solution at the same time, so that the nitrobenzene content in the medium reaches the initial concentratio...

Embodiment 2

[0028] The bacterial agent for repairing organically polluted water bodies is composed of facultative anaerobic microorganisms, and the facultative anaerobic microorganisms can be: Pseudomonas stutzeri, Alealigenesfaecalis, Bacillus megaterium ), Enterobacter cloacae (Enterobacter cloacae), the ratio of parts by weight is 1:1:2:6, and the balance is the culture solution containing nitrobenzene.

[0029] The preparation method of bacterium agent: the facultative anaerobic microorganism that is 5% by weight is inoculated in the anaerobic bottle that contains the sterilized culture fluid of 5ml nitrobenzene that 400ml is housed, and the initial concentration of nitrobenzene reaches 80mg in the culture medium / L, sealed, and placed in a 30°C incubator for 4 days, then take out 1 / 5 of the culture solution, add the same amount of culture solution and add nitrobenzene solution at the same time, so that the content of nitrobenzene in the medium reaches the initial concentration , and ...

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Abstract

The invention relates to the microbial field, in particular to a microorganism agent for recovering the organic polluted water and preparation process and using method. The microorganism agent consists of facultative anaerobics including Pseudomonas, Alcaligenes, Bacillus and Enterbacter with the weight ratio of 1:1-3:1-4:2-7, and the residual is the culture liquid containing nitrobenzene; the degradation rate can reach between 97.7 percent and 99.6 percent when the invention is implemented in the polluted water containing nitrobenzene. The microorganism agent of the invention is made by that the nitryl aromatic hydrocarbon compound and the nutrition compound with relatively high concentration are acclimatized and sorted, and has strong growing and reproductive ability as well as adaptability; in addition, the invention can assure a higher biomass in the environment at low cost, and does not cause the secondary pollution, and has the effect of continuously strengthening the purification of the water, thereby having good social, economic and ecological benefits.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a bacterial agent for repairing organically polluted water bodies and its preparation and use methods. Background technique [0002] The pollution of natural water bodies (including sediment) with cities as the center and rivers as the link caused by the rapid development of industry and agriculture is an environmental problem that the world expects to solve. A large amount of industrial waste and accidents produced by my country's chemical and petrochemical industries can easily cause pollution of drinking water sources along rivers, lakes and seas. The nitrobenzene pollution accident in Jihua's double benzene workshop seriously polluted the water body of Songhua River and caused huge international impact. A chemical group in Liaoning discharges more than 7 million tons of waste water containing nitrobenzene compounds every year, and its pollution level far exceeds the pollution c...

Claims

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Application Information

Patent Timeline
02 Apr 2008
Publication
CN101153271A
IPC
C12N1/20; C02F3/34; C12R1/07; C12R1/05
Inventors
李培军; 许华夏