Microbial inoculum for repairing amino benzenes polluted water and its preparation method and using method

A bacterial agent, aniline technology, applied in the field of microorganisms, can solve the problems of the reduction of the total amount of degrading bacteria, the slow degradation of environmental pollutants, the inability to continue to degrade pollutants, etc., and to meet the requirements of environmental conditions that are not harsh, reproductive capacity and adaptability. Strong and continuous enhancement of the effect of water purification

Inactive Publication Date: 2008-04-23
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it cannot fully meet the actual needs. This is because the pollutants scattered in the natural environment cannot be treated centrally and uniformly like industrial wastewater; the composition of the compounds in the polluted environment changes greatly, and the pH fluctuation is also large, which may inhibit degradation. The growth of degrading bacteria; the degradation rate of degrading bacteria to environmental pollutants is slow, which cannot meet the requirements of actual needs or often mutates and cannot continue to degrade pollutants; the polluted environment cannot effectively maintain the biomass of degrading bacteria. A reduction in the total amount of degrading bacteria often occurs when bacteria

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The bacterial agent is composed of aerobic microorganisms, the aerobic microorganisms are: Pseudomonas fluorescens, Alealigenes eutrophus, Zoogloea ramigera, Aeromonas hydrophila (Aeromonas hydrophila), simple knot bacteria (Arthrobacter simplex) and gigantic bacillus (Bacillus megaterium), its weight ratio is 10:3:2:1:1:1, and the balance is the culture solution containing aniline.

[0022] The preparation method of bacterium agent: the aerobic microorganism bacterium liquid that is 10% by weight is inoculated in the Erlenmeyer flask that 150ml sterilized culture solution is housed, and the aniline initial concentration reaches 30mg / L in the culture medium, gauze pad seals, and places 28 ℃ in a constant temperature shaking incubator at a frequency of 115r / min for 3 days, then take out 1 / 3 of the culture solution, and then add the same amount of culture solution to make the initial concentration of aniline in the medium reach 30mg / L, and continue in the incubator Cultiv...

Embodiment 2

[0029] The bacterial agent is composed of aerobic microorganisms, and the aerobic microorganisms are:

[0030] Pseudomonas aeruginosa, Alealigenesfaecalis, Zoogloea ramigera, Aeromonas hydrophila, Arthrobacter simplex and Bacillus megaterium ( Bacillus megaterium), the weight ratio is 8:4:1:3:2:2, and the balance is the culture solution containing nitrobenzene.

[0031] The culture solution is calculated by weight percentage: glucose 0.05%, peptone 0.02%, beef extract 0.03%, NaCl 0.3%, NaCl 2 HPO 40 0.3%, KH2 PO 4 0.1%, MgSO 4 .7H 2 O0.02%, pH7.5.

[0032] The preparation method of the bacterial agent: the bacterial agent of 5% by weight is inoculated in a conical flask equipped with 150ml of sterilized culture solution, the initial concentration of aniline in the culture solution is 80mg / L, sealed with a gauze pad, and placed at a constant temperature of 28°C Shake culture at a frequency of 140r / min in a shaking incubator for 4 days, then take out 1 / 4 of the culture sol...

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Abstract

The present invention relates to microbiology technology, and is especially microbial preparation for repairing aniline contaminated water and its preparation process and usage. The microbial preparation consists of aerobes Pseudomonas, Alealigenes, Zoogloea, Aeromonas, Arthrobacter and Bacillus in the weight ratio of 8-10 to 3-4 to 1-2 to 1-3 to 1-2 to 1-2, as well as culture liquid containing aniline. It is applied into aniline contaminated water to reach aniline degrading rate up to 80-100 %. It is prepared through domestication in relatively high concentration nitro arene compound and nutritious compound and screening. It has high growth and reproduction capacity, powerful adaptability, high biomass, low cost, powerful water purifying effect and no secondary pollution.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a bacterial agent for repairing aniline-polluted water bodies and a preparation method thereof. Background technique [0002] Aniline is a widely used chemical raw material, mainly used in national defense, printing and dyeing, rubber, paint, pesticide and pharmaceutical industries, etc. It is a harmful substance that seriously pollutes the environment and endangers human health, and is carcinogenic. Due to the toxicity of aniline compounds to organisms, people have always paid attention to them. In my country, aniline compounds are listed as key pollutants under priority control in the environment, and industrial drainage is strictly managed, and the highest allowable discharge standard has been formulated, with a concentration of 5mg / L. The drinking water standard is only 0.2mg / L. [0003] The treatment of water bodies polluted by aniline compounds is difficult, and there are fe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/38C12R1/05C12R1/07
Inventor 李培军许华夏张春桂张海荣尹炜鞠京丽
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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