Alcaligenes faecalis, method for preparation of desulfurization deodorant from the same and application

A technology of Alcaligenes faecalis and deodorant, which is applied in the field of Alcaligenes faecalis and its application in environmental deodorization, and can solve the problems of difficulty in obtaining high-density bacteria, increasing the cost of deodorization reaction, and long metabolic time of strains, etc. no secondary pollution, solve the problem of odor, and quickly deodorize

Active Publication Date: 2013-03-13
BEIJING AGRO BIOTECH RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In biological deodorization, autotrophic sulfur-oxidizing bacteria are often used in the existing technology to remove hydrogen sulfide. The disadvantages are that the strains have long metabolism time, slow growth, and it is difficult to obtain high-density bacteria. The existing heterotrophic sulfur-oxidizing bacteria need regular Nutrients are added, which increases the cost o

Method used

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  • Alcaligenes faecalis, method for preparation of desulfurization deodorant from the same and application
  • Alcaligenes faecalis, method for preparation of desulfurization deodorant from the same and application
  • Alcaligenes faecalis, method for preparation of desulfurization deodorant from the same and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Isolation and identification of Alcaligenes faecalis M50-B1 strain

[0036] 1. Isolation of strains

[0037]The source of isolation comes from the sawdust on the surface of the compost biofilter. The sample was inserted into 100ml heterotrophic sulfur oxidizing bacteria screening medium according to the inoculation amount of 5% (V / V), and placed in a constant temperature shaker for cultivation. The temperature was controlled at 30°C, 120r / min, regularly (7 days) transferred to fresh medium to continue enrichment. After 21 days of acclimatization, the sulfur oxidation efficiency of the samples after 3 consecutive passages was measured, and it was obtained that the metabolic efficiency reached more than 90%. The obtained enriched culture solution was serially diluted and spread onto a solid plate of heterotrophic sulfur oxidizing bacteria culture medium, cultured at 30°C for 7 days, and single colonies were picked and streaked for separation and purification t...

Embodiment 2

[0047] Embodiment 2 Preparation and use of liquid desulfurization and deodorant

[0048] (1) Under aseptic conditions, pick 1-2 rings of Alcaligenes faecalis M50-B1 strain stored in a glycerol tube at -80°C, and streak on a fresh heterotrophic sulfur-oxidizing bacteria solid medium plate. Lines were cultured at 28°C for 3 days.

[0049] (2) Pick one ring of the culture from (1) and place it in 150 ml of heterotrophic sulfur-oxidizing liquid bacterial culture medium at 28°C for shaking culture on a shaker, and culture for 26 hours to obtain a seed liquid. Add 5L of water to a 7L small fermenter, and add 15g of beef extract, 45g of peptone, 15g of yeast extract, Na 2 HPO 4 12H 2 O 10g, NaCl 15g, 1mol / L hydrochloric acid to adjust the pH to 7.0, sterilize at 121°C for 20min. When the temperature of the culture medium drops to 28°C, inoculate the seed liquid in the fermenter at a volume ratio of 2%, at a temperature of 28°C, with an air flow of 5 L / min, and a stirring speed of...

Embodiment 3

[0052] Embodiment 3 Preparation and use of solid desulfurization deodorant

[0053] (1) Strain activation: Under sterile conditions, pick 1-2 rings of Alcaligenes faecalis M50-B1 strain stored in a glycerol tube at -80°C, and culture them on fresh heterotrophic sulfur oxidizing bacteria solid Streak on the basal plate and incubate at 30°C for 2 days.

[0054] (2) Pick one ring of the culture from (1) and culture it on a shaker at 30° C. in 50-150 mL of heterotrophic sulfur-oxidizing liquid bacterial culture medium, and culture it for 25 hours to obtain a seed liquid.

[0055] (3) Inoculate the above-mentioned seed liquid into a fermenter containing freshly sterilized heterotrophic sulfur-oxidizing liquid bacterial culture medium at a volume ratio of 5%, and ferment and cultivate at 28°C with a tank pressure of 0.05Mpa and an air flow of 80L / min 1. The stirring speed is 150r / min, add 40mL foam enemy as a defoamer, take a sample every two hours, measure the absorbance at 600nm ...

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Abstract

The invention provides an Alcaligenes faecalis strain M50-B1, which has a preservation number of CGMCC 5219. The invention also provides application of the bacterium in a biological desulfurization deodorant. The bacterium is prepared into a liquid bacterial agent and a solid bacterial agent. During oxidation of hydrogen sulfide, no acid is produced. At the same time, a weak acidic bacterial liquid can absorb the ammonia in the air in the spraying process, thus achieving a good deodorization effect. The activated carbon in solid bacterial agents makes up the delay defect of biological deodorization, and the deodorizing cost is reduced. The Alcaligenes faecalis provided by the invention and the biological desulfurization deodorant prepared from the same have a good deodorization effect. The desulfurization deodorant has the advantages of simple preparation method, low cost, and no secondary pollution, thus being suitable for popularization and application in deodorization of household garbage and livestock and poultry production.

Description

technical field [0001] The invention relates to the field of biological fermentation, in particular to Alcaligenes faecalis and its application in environmental deodorization. Background technique [0002] There are a large number of odor-causing microorganisms in poultry manure and domestic waste, which can degrade sulfur-containing organic matter, especially protein, thereby releasing hydrogen sulfide and producing stench. With the development of large-scale and intensive animal husbandry in rural areas, the pollution of hydrogen sulfide produced in livestock and poultry production to the environment continues to intensify. As one of the main components of odor, hydrogen sulfide can stimulate people's olfactory nerve and trigeminal nerve, and poison the respiratory center. It not only poses a great threat to human health, causes air pollution, but also seriously affects livestock (poultry) ) health and production performance, so the hydrogen sulfide pollution in the odor ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61L9/01C12R1/05A61L101/52
Inventor 仇天雷李焕王旭明孙晓红邢礼军王彦珍韩梅琳
Owner BEIJING AGRO BIOTECH RES CENT
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