Selenium-rich combination azotobacter and preparation method for freezing dry powder, liquid fertilizer and solid fertilizer of the same

Abstract

The present invention provides a method for producing selenium-enriched combined nitrogen-fixing bacteria agent and its freeze-dry powder, liquid fertilizer and solid fertilizer. The culture medium of selenium-enriched combined nitrogen-fixing bacteria is composed of Na2SeO3, peptone, yeast, rare earth aqueous solution, NaCl, water etc. Three strains are respectively inoculated in flasks. Alcaligenes faecalis is represented by A15, nitrogen-fixing giant bacillus is represented by N13, Enterobacter Cloacae is represented by E26. Liquid bacterial fertilizer is obtained via rocking bottle enlarged cultivation, seed tank culture, liquid ferment in ferment tank, preservation and adjusting pH. If it is processed with vacuum freeze-dry, the freeze-dry powder is obtained. The solid fertilizer culture medium comprises grass charcoal, chicken dung, gypsum, rare earth, KH2PO4,Na2SeO3 5H2O. Fermented bacteria strain in ferment tank is inoculated at solid fertilizer culture medium and the final product is obtained after compost. The present inventive process is practical and suitable for industrial production.

Description

technical field [0001] The invention relates to the technical field of combined nitrogen-fixing engineering bacteria, in particular to a method for preparing selenium-enriched combined nitrogen-fixing bacteria and freeze-dried powder, liquid and solid fertilizers. Background technique [0002] The development of nitrogen-fixing bacterial fertilizer is a scientific and technological revolution with far-reaching influence on the construction of new agriculture. While promoting the growth of crops, improving their stress resistance and increasing production, it can also overcome the compaction and desertification caused by chemical fertilizers on the soil. , environmental protection, green food and other fields will have a positive impact. With the acceleration of the implementation of the great project of building modern agriculture, the development of the joint solid-state nitrogen bacteria fertilizer presents a bright prospect. However, due to the cond...

AI Technical Summary

Problems solved by technology

[0002] The development of nitrogen-fixing bacterial fertilizer is a scientific and technological revolution with far-reaching influence on the construction of new agriculture. While promoting the growth of crops, improving their stress resistance and increasing production, it can also overcome the compaction and desertification caused by chemical fertilizers on the soil. , environmental protection, green food and other fields will have a positive impact. With the acceleration of the implementation of the great project of building modern agriculture, the development of the joint solid-state nitrogen bacteria fertilizer presents a bright prospect. However, due to the conditions of the joint nitrogen-fixing bacteria fertilizer itself Due to the limitation, it has obvious effects on the stress resistance, growth promotion and yield increase of crops, but it is inferior to improving the quality of crops such as fitness and health care functions. With the continuous improvement of people's quality of life, people's requirements for crops are increasing. While satisfying their basic physiological needs, they hope to receive the effect of physical fitness and health care