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Alcaligenes faecalis and application thereof

A technology of Alcaligenes faecalis and microbial strains, applied to Alcaligenes faecalis and its application fields, can solve problems such as physical harm, pollution of groundwater sources, pollution of the environment and the like

Active Publication Date: 2018-12-11
SHAANXI SCI TECH UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although biological desulfurization technology has many benefits, there are very few bacterial strains that can destroy benzothiophenes, and it is still in the initial stage of research and development
[0004] At the same time, with the continuous improvement of people's living standards, livestock manure and domestic garbage are also increasing day by day, and the odor pollutants produced are increasing. A large amount of ammonia and hydrogen sulfide will be produced in the process of landfill and composting, which is not only harmful to people cause serious harm to the body, pollute the environment, pollute groundwater sources, etc.

Method used

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  • Alcaligenes faecalis and application thereof
  • Alcaligenes faecalis and application thereof
  • Alcaligenes faecalis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment one: collection and identification of bacterial strain

[0036] 1. Collection: Take the sludge from the secondary settling tank of a sewage treatment plant in Sichuan and mix it with compost samples. The collected activated sludge is mixed with the compost sample, and the volume is 10% of the enriched medium, inoculated in the enriched medium, and acclimatized with the enriched medium at 30°C under aerobic conditions, and acclimatized for 5 days per generation , a total of 4 generations of domestication for a total of 20 days, followed by serial dilution, from 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 、10 -8 Draw 0.25mL at the dilution, spread it on the isolation medium plate, and cultivate it at a constant temperature of 30°C. After multiple isolations, several strains are finally obtained.

[0037] The compost sample specifically refers to the direct stacking of common crop by-products (such as straw, rice husk, etc.) for 150 days or more.

[0038] 2...

Embodiment 2

[0049] Embodiment two: the optimal condition of bacteria strain degrading ammonia nitrogen substance

[0050] The inventors found in experiments that the strain has excellent performance in degrading ammonia nitrogen, and can be practically applied to the treatment of ammonia nitrogen substances. In this example, all the culture media used were adjusted accordingly on the basis of the separation culture medium.

[0051] Inoculate the isolated and purified bacterial strain into beef extract peptone medium according to the inoculum size as one inoculation loop, and culture at 35° C. for 24 hours to obtain the seed solution.

[0052] 1. Effect of C / N on the degradation of ammonia nitrogen

[0053] C / N refers to the mass ratio of C and N in the medium.

[0054] Inoculate the seed liquid into the culture medium with C / N ratios of 2, 5, 10, 15 and 20 respectively according to the volume of 1% of the medium volume, and measure the degradation rate and OD of the strain to ammonia ni...

Embodiment 3

[0066] Example 3: Degradation of high ammonia nitrogen wastewater by bacterial strains

[0067] 1. The seed solution prepared in Example 2 was inoculated into the liquid expansion fermentation medium according to the inoculation amount of 1% of the volume of the liquid expansion fermentation medium, and shaken at 35° C. and 160 rpm for 24 hours to obtain the inoculum.

[0068] 2. Inoculate 5ml of the inoculation solution into a 1L beaker (inoculum size is 1.0%) with 500ml biogas slurry high ammonia nitrogen wastewater (initial ammonia nitrogen concentration is 416.2mg / L), and add an equal amount of distilled water as a control group. Three repetitions were set for each group, the temperature was kept at 30°C, and the concentration of ammonia nitrogen was measured every 6h. The results were as follows: Figure 5 shown.

[0069] 3. The results show that: under the condition that the initial ammonia nitrogen concentration is 416.2mg / L, the strain can degrade ammonia nitrogen to ...

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Abstract

The invention belongs to the field of microbial application, and particularly relates to alcaligenes faecalis and application thereof. According to the specific technical scheme, the alcaligenes faecalis is preserved in China General Microbiological Culture Collection Center on June 11, 2018, the strain name is alcaligenes faecalis DFN2, and the preservation number is CGMCC No.15926. The alcaligenes faecalis has an excellent degradation effect on common malodorous substances such as ammonia gas and hydrogen sulfide, and the maximum degradation rate of the ammonia gas and the hydrogen sulfide can reach 100%; and the degradation rate of an ammonia nitrogen substance in high-ammonia-nitrogen wastewater can reach up to 99.5%. The alcaligenes faecalis can also carry out biological desulfurization treatment on thiophene sulfide, and the desulfurization effect is obviously superior to a universal hydrodesulfurization effect.

Description

technical field [0001] The invention belongs to the field of microorganism application, and in particular relates to a strain of Alcaligenes faecalis and its application. Background technique [0002] With the increasing demand for diesel oil, my country has continuously increased the conversion capacity of residual oil, and the sulfur content of diesel oil has also increased. At present, the method of hydrodesulfurization is mainly used. Although this process can increase the desulfurization efficiency, some naphthalene-based aromatics are hydrogenated to form tetrahydronaphthalene, which makes the oil unstable and increases the cost of desulfurization. At the same time, the desulfurization method requires relatively low reaction conditions. higher, and the cost is relatively higher. [0003] At present, many countries have begun to devote themselves to the research of biological desulfurization technology. Biological desulfurization technology can be carried out at norma...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02B01D53/84B01D53/52B01D53/58C02F3/34C12R1/05C02F101/16
CPCB01D53/52B01D53/58B01D53/84C02F3/34C02F2101/16C12N1/02C12N1/205C12R2001/05Y02A50/20
Inventor 梁引库闫志英宦臣臣
Owner SHAANXI SCI TECH UNIV
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