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Microbial fertilizer and preparation method thereof

A technology of microbial fertilizers and mixtures, applied in the direction of organic fertilizers, etc., can solve the problems of insufficient research and development of PGPR, and achieve the effects of improving plant disease resistance, short production cycle, and easy promotion

Active Publication Date: 2012-07-25
XIAN UNVERSITY OF ARTS & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But not enough for other PGPR research and development

Method used

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  • Microbial fertilizer and preparation method thereof
  • Microbial fertilizer and preparation method thereof
  • Microbial fertilizer and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] The microbial fertilizer of the present embodiment, the microbial fertilizer is that the content of hydrogen oxidizing bacteria is 1×10 9 each / mL of fermentation broth; the hydrogen oxidizing bacteria is Flavobacterium johnsonii.

[0030] The preparation method of the microbial fertilizer of the present embodiment comprises the following steps:

[0031] Step 1: Inoculate Flavobacterium johnsonii in a solid medium for cultivation to obtain a first-class strain; the solid medium is MSA solid medium, the cultivation temperature is 30°C, and the cultivation time is 36h;

[0032] Step 2, inoculating the first-level strains in step 1 into a liquid medium for cultivation to obtain second-level strains; the liquid medium is MSA liquid medium, the culture temperature is 30°C, and the culture time is 72h;

[0033] Step 3, inoculate the secondary bacterial species described in Step 2 into a 2.5L fermentation medium with an inoculum size of 5% to obtain a fermentation broth of Fla...

Embodiment 2

[0036] This embodiment is the same as Example 1, wherein the difference is that the hydrogen oxidizing bacteria are Pseudomonas, Pseudomonas corrugae, Alcaligenes argus or Burkholderia cepacia, or Flavobacterium johnsonii, At least two of Pseudomonas, Pseudomonas, Alcaligenes, and Burkholderia cepacia.

Embodiment 3

[0038] The microbial fertilizer of the present embodiment, described microbial fertilizer is that hydrogen oxidizing bacteria content is 1 * 10 8 Individual / mL fermentation broth; the hydrogen oxidizing bacteria are Flavobacterium johnsonii and Pseudomonas corrugatum.

[0039] The preparation method of the microbial fertilizer of the present embodiment may further comprise the steps:

[0040] Step 1, respectively inoculating Flavobacterium johnsonii and Pseudomonas versicolor in a solid medium for cultivation to obtain a first-grade strain; the solid medium is an MSA solid medium;

[0041] Step 2, respectively inoculating the primary bacterial strain described in step 1 in a liquid medium for cultivation to obtain a secondary bacterial strain; the liquid medium is an LB liquid medium;

[0042] Step 3, respectively inoculate the secondary bacterial classification described in step 2 in 2.5L fermentation medium and carry out fermentation culture, obtain Flavobacterium johnsonii...

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Abstract

The invention discloses microbial fertilizer and a preparation method thereof. The microbial fertilizer is fermentation liquor with hydrogen-oxidizing bacterium content ranging from 1X108 / mL to 1X1010 / mL or mixture of hydrogen-oxidizing bacterium liquid and sterile grass carbon, the content of hydrogen-oxidizing bacteria in the mixture ranges from 2X108 / g to 2X1010 / g, moisture of the mixture ranges from 20wt% to 40wt%, and the hydrogen-oxidizing bacteria include one type or a plurality of types of a flavobacterium, a pseudomonas, alcaligenes and burkholderia. The invention further provides a preparation method of the microbial fertilizer. The microbial fertilizer realizes a fine application effect for plants, and is free of environmental pollution, low in consumption and easy in popularization, the preparation process of the microbial fertilizer is simple, the production cycle is short, and cost is low.

Description

technical field [0001] The invention belongs to the technical field of microbial fertilizers, and in particular relates to a microbial fertilizer and a preparation method thereof. Background technique [0002] Because of its ability to improve soil, improve soil fertility, improve the absorption and utilization of plant mineral elements, enhance the disease resistance and drought resistance of crops, increase yield, and improve quality, microbial fertilizers are used in the development of green agriculture, ecological agriculture, and the production of safe and pollution-free agricultural products. It shows an irreplaceable role. In particular, Plant Growth Promoting Rhizobacteria (PGPR for short) directly or indirectly promotes plant growth through one or more growth-promoting mechanisms. [0003] At present, microbial fertilizers are mostly concentrated in rhizobia fertilizers, nitrogen-fixing bacteria fertilizers, phosphorus-solubilizing fungi fertilizers, and potassium-...

Claims

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Application Information

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IPC IPC(8): C05F11/08
Inventor 赵银萍董忠民陈克克丁群英梁振荣折小蕊符鹏
Owner XIAN UNVERSITY OF ARTS & SCI
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