Alcaligenes faecalis YS302 and application thereof

A technology of Alcaligenes faecalis, YS302, applied in bacteria, biochemical equipment and methods, microorganisms, etc., can solve the problems of not being able to remove ammonia nitrogen and hydrogen sulfide at the same time, polluting groundwater sources, etc., to achieve good methods and technical support, reduce cost, cost reduction effect

Active Publication Date: 2017-05-31
BEIJING UNIV OF AGRI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the landfill will produce a large amount of leachate during the period of use until it is scrapped, and the ammonia nitrogen content in the landfill leachate is getting higher and higher. If it is not treated, the landfill leachate will continue to infiltrate and pollute the groundwater source
[0005] At present, in the traditional sense, the same strain cannot simultaneously remove ammonia nitrogen and hydrogen sulfide in sewage and odor

Method used

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  • Alcaligenes faecalis YS302 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Isolation and identification of Alcaligenes faecalis YS302

[0022] 1. Isolation and purification of Alcaligenes faecalis YS302

[0023] The landfill leachate was obtained from Nangong Food Waste Treatment Plant, and the landfill leachate was added to the enrichment medium. The preparation of the enrichment medium was as follows: beef extract 5.0g, egg white 10.0g, sodium thiosulfate pentahydrate 10.0 g, ammonium chloride 4g, water 1000ml, pH value 6.0-8.0, prepare 100ml of separation medium, add 10ml of landfill leachate, and then enrich, add 0.3g of sodium sulfide nonahydrate every other day, enrich for 1 week .

[0024] The enriched culture medium is diluted up to 10,000 times by the gradient dilution method, and then inserted into the separation medium, and then a single colony is picked out from the separation plate for streak purification, and the purification is repeated 4 to 5 times, and the obtained pure The colonies of species were drawn and cultu...

Embodiment 2

[0028] Example 2: Experimental verification of simultaneous degradation of ammonia nitrogen and hydrogen sulfide by Alcaligenes faecalis YS302

[0029]Pick the purified YS302 single colony and inoculate it into the enrichment medium for fermentation. The enrichment medium is prepared as follows: 5.0g of beef extract, 5.0g of egg white, 10.0g of sodium thiosulfate pentahydrate, 4.0g of ammonium chloride, Water 1000ml, pH value 6.0-8.0; control temperature 25-30°C, pH 6-8, rotating speed 150-200rpm, fermentation time 36-72 hours, to obtain the mother liquor of biological bacteria degradation agent; The proportion of the ammonia nitrogen in the screening medium was added to the screening medium, wherein the initial concentration of ammonia nitrogen in the screening medium was prepared as three gradients of 500mg / L, 1000mg / L, and 1500mg / L respectively, and the screening medium of the three gradients was prepared as follows: Ammonium chloride 1.5 / 3 / 4.5g, sodium thiosulfate pentahy...

Embodiment 3

[0031] Embodiment 3: the preparation of biological bacteria degradation agent

[0032] (1) Inoculate the purified Alcaligenes faecalis YS302 into the enrichment medium for fermentation and culture, control the temperature at 25-30°C, pH 6-8, rotation speed at 150-200rpm, and enrichment time for 36-72 hours to obtain biological Bacteria degrading agent mother liquor; the fermentation medium is prepared as follows: beef extract 5.0-10.0g, egg white 5.0-10.0g, sodium thiosulfate pentahydrate 4.0-10.0g, ammonium chloride 0.4-4.0g, water 1000ml, pH value 6.0~8.0.

[0033] (2) Then, the biological bacteria degradation agent mother liquor is added to the fermentation medium according to the ratio of 0.5% for fermentation and cultivation, the temperature is controlled at 25-30°C, the pH is 6-8, the rotation speed is 150-200rpm, and the fermentation time is 36-72 hours, and the obtained Biodegradant, fermentation medium prepared as follows: ammonium chloride 1.5-4.5g, sodium thiosulfa...

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Abstract

The invention discloses alcaligenes faecalis (Alcaligenes faecalis) YS302. The alcaligenes faecalis YS302 is collected in China General Microbiological Culture Collection Center (called CGMCC for short) on December 26, 2016 and has a collection number of CGMCC NO. 13502. The invention further discloses application of the alcaligenes faecalis. The invention has the advantages that only the alcaligenes faecalis YS302 needs to be added, extra packing does not need to be added, ammonia nitrogen and hydrogen sulfide in sewage and stench can be simultaneously degraded, the sewage treatment cost is reduced, the cost and space and time needed for treatment can be further reduced, and a good method and technical support can be provided for environmental workers.

Description

technical field [0001] The invention relates to Alcaligenes faecalis YS302 and its application. Background technique [0002] At present, the main methods of centralized disposal of urban waste in my country are urban waste landfill and composting. A large amount of ammonia and hydrogen sulfide are produced in the process of landfill and composting, and the petroleum industry and coal industry will produce high concentrations of hydrogen sulfide and other pollutants, and these high concentrations of ammonia and hydrogen sulfide have a pungent smell And corrosive, not only cause serious harm to the human body, but also pollute the environment, pollute groundwater sources. [0003] Hydrogen sulfide is a kind of nerve poison, which has the smell of rotten eggs. It is the main component of malodorous gas and one of the most toxic pollutants in air pollutants. of good health. Low concentration of hydrogen sulfide will produce more obvious stimulating effects, such as tearing, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B01D53/84B01D53/52B01D53/54C02F11/02C12R1/05
CPCB01D53/52B01D53/54B01D53/84C02F11/02C12N1/20Y02A50/20
Inventor 靳永胜秦文靳静晨郝芳华罗胜南杨祎尚润东张闻天
Owner BEIJING UNIV OF AGRI
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