Alcaligenes faecalis and application thereof

A technology of Alcaligenes faecalis and microbial strains, applied to a strain of Alcaligenes faecalis and its application fields, can solve the problems of increased manpower and capital investment, long reactor start-up time, and increased water treatment costs, and achieve shortening The effect of starting time, reducing construction funds, and reducing floor space

Inactive Publication Date: 2013-09-11
CHONGQING UNIV
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  • Abstract
  • Description
  • Claims
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Benefits of technology

The Alcaligenes faecalis strain described in this patent has several advantages for wastewater treatment: 1. It can remove both nitrogen and organic carbon simultaneously. It can convert organic nitrogen or NH4+ into gaseous products like N2O and N2 under aerobic conditions. It can also effectively remove organic carbon in wastewater. This means that only one stage of aerobic treatment is needed to remove both nitrogen and organic carbon, with removal rates of around 80-90% for organic carbon and 78-88% for TN. 2. Using this strain eliminates the need for separate aerobic nitrification and anoxic denitrification processes, reducing space and costs. 3. The denitrification process is carried out by a single heterotrophic bacteria, avoiding the need for a combination of autotrophic nitrifying bacteria and heterotrophic denitrifying bacteria. This strain has a fast growth rate, shortening system startup time and improving treatment efficiency. 4. The strain can tolerate high concentrations of organic carbon and nitrogen sources, making it suitable for treating high C/N ratio organic wastewater. It can achieve 100% removal of ammonia nitrogen and over 80% removal of TOC in high ammonia nitrogen and high organic carbon wastewater. In summary, the application of this Alcaligenes faecalis strain can improve the efficiency and economic benefits of traditional wastewater treatment processes, with broad application potential and positive social and environmental benefits.

Problems solved by technology

Technology Problem addressed in this patented technical problem relates to developing effective methods or agents capable of effectively degradating harmful substances in aquatic systems containing excessive amounts of organics called nitroorganilbons, including butterferon nitrosamines, cyclohexane diazotinitrilum salts, etc., without causing secondary pollution. Current separation techniques involve physical separators like settling tanks and membranes, leading to complexity and cost issues associated with existing purifications.

Method used

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  • Alcaligenes faecalis and application thereof
  • Alcaligenes faecalis and application thereof
  • Alcaligenes faecalis and application thereof

Examples

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Embodiment approach 1

[0049] Embodiment 1: Alcaligenes faecalis ( Alcaligenes faecalis ) NR on the removal effect of total organic carbon (TOC) and total nitrogen (TN) in wastewater from different organic carbon sources

[0050] Alcaligenes faecalis NR were placed in simulated wastewater containing different organic carbon sources. The initial organic carbon concentration (TOC) in each wastewater was 800 mg / L, and the total nitrogen (TN) concentration was about 80 mg / L. At 30°C, Treated for 72h under the condition of pH 7. Depend on figure 2 It can be seen that the strain NR can use a variety of organic carbon sources, and the removal rate of organic carbon is about 80% to 90%. Among them, the removal effect on malic acid is the best, and the removal rate of TOC reaches more than 93%. While removing organic carbon, it has a good removal effect on nitrogen in various wastewaters, and the removal rate of TN can reach about 78%~88%. It shows that the strain NR has a good simultaneous removal effec...

Embodiment approach 2

[0051] Embodiment 2: Alcaligenes faecalis ( Alcaligenes faecalis ) NR for ammonia nitrogen organic wastewater treatment

[0052] Place Alcaligenes faecalis NR in NH 4 + The removal efficiency of the strain NR for ammonia nitrogen and total nitrogen was investigated in the artificially distributed water as the only nitrogen source. Artificial water distribution is (per liter): NH 4 Cl 0.688 g, glucose 4.5 g, NaCl 4 g, NaCl 2 HPO 4 2.66 g, KH 2 PO 4 1 g, trace element solution 3 mL. Each liter of trace element solution contains MgSO 4 ·7H 2 O 3 g, MnSO 4 3 g, ZnSO 4 ·7H 2 O 3 g, H 3 BO 3 1.12 g, FeSO 4 ·7H 2 O 0.3 g, CaCl 2 0.6 g. The initial concentration of ammonia nitrogen in artificial water distribution was 180 mg / L, and the C / N ratio was 10. The culture conditions were 30°C and 120 rpm. Growth of strains NR, NH 4 + , TN and TOC removal such as image 3 shown. Strain NR grew well in the artificial water distribution, reaching the maximum gro...

Embodiment approach 3

[0053] Embodiment 3: Alcaligenes faecalis ( Alcaligenes faecalis ) NR applied to urea-containing wastewater

[0054] In order to investigate the removal of organic nitrogen source by Alcaligenes faecalis NR, the strain NR was added to the artificial water containing urea. The water quality is (per liter): urea 0.17 g, glucose 2 g, NaCl 4 g, Na 2 HPO 4 2.66 g, KH 2 PO 4 1 g, trace element solution 3 mL. Each liter of trace element solution contains MgSO 4 ·7H 2 O 3 g, MnSO 4 3 g, ZnSO 4 ·7H 2 O 3 g, H 3 BO 3 1.12 g, FeSO 4 ·7H 2 O 0.3 g, CaCl 2 0.6 g. Cultured at 30°C and 120 rpm for 72 h, the growth of strain NR in urea-containing wastewater and the removal of TN and TOC are shown in Figure 4 . It can be seen from the figure that Alcaligenes faecalis NR can grow in urea-containing wastewater, the removal rate of TN is 86.9%, and the removal rate of TOC is 92.5%. It shows that Alcaligenes faecalis NR not only has efficient removal ability for inorganic n...

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Abstract

The invention discloses an alcaligenes faecalis and application thereof. The alcaligenes faecalis is named alcaligenes faecalis (Alcaligenesfaecalis) NR and is stored in the China General Microbiological Culture Collection Center (CGMCC) with CGMCCNo.7543 on April 28, 2013. The alcaligenes faecalis is applicable to the treatment of nitrogen-containing organic wastewater and can be used for synchronously removing nitrogen and organic carbon in the wastewater. The alcaligenesfaecalis NR has both functions of denitriding and removing the organic carbon synchronously, only needs one aerobic stage to realize the synchronous removal of the organic carbon and nitrogen, and has the removal rate of about 80 to 90% for the organic ratio, and the removal rate of about 78 to 88% for TN (Total Nitrogen).

Description

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Claims

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Application Information

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Owner CHONGQING UNIV
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