Composite microbial biogen capable of degrading organic waste efficiently and preparation method and applications thereof

A technology for compound microorganisms and live bacteria preparations is applied in the field of compound microorganism live bacteria preparations for efficiently degrading organic wastes and its preparation, which can solve the problems of reduction and large sludge discharge, and achieves inhibition of reproduction, reduction of production volume, and elimination of peculiar smells. Effect

Inactive Publication Date: 2011-05-25
WUXI ZHONGJIA TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Therefore, the technical problem to be solved in the present invention is to provide a kind of compound microbial live bacteria preparation for efficiently degrading organic waste and its preparation method and application in view of the existing sewage treatment technology that has a large amo

Method used

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  • Composite microbial biogen capable of degrading organic waste efficiently and preparation method and applications thereof
  • Composite microbial biogen capable of degrading organic waste efficiently and preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Carry out amplified culture respectively:

[0039] Bacillus circulans (Bacillus circulans) CGMCC 1.554 was activated on a slope, inoculated in a liquid medium, and cultured at 35° C. for 48 hours. Liquid medium formula: glucose 3%, tryptone 1.5%, beef extract 3%, peptone 2%, Tween 800.1%, sodium acetate 0.5%, ammonium citrate 0.2%, magnesium sulfate 0.058%, manganese sulfate 0.25%, pH6 .5, percentage is weight volume percentage, ie g / 100ml.

[0040] Bacillus subtilis (Bacillus subtilis) CGMCC 1.308 and activated slant were inoculated in liquid medium and cultured at 28°C for 48 hours. Liquid medium formula: 1.5% of soybean powder, 1% of starch, 0.2% of yeast extract, 1% of glucose, pH6.5, and the percentages are weight and volume percentages.

[0041] Alcaligenes faecalis (Alcaligenes faecalis) CGMCC 1.924 and Penicillium diversum (Penicillium diversum) ATCC 10437 were activated on the slant, respectively inoculated in liquid medium, and cultured at 30°C for 48 ho...

Embodiment 2

[0045] (1) Carry out amplified culture respectively:

[0046] Bacillus circulans (Bacillus circulans) CGMCC 1.383, ATCC 13403, ATCC15518 and ATCC 21656 were activated on slant planes, inoculated in liquid medium, and cultured at 37°C for 44 hours. Liquid culture medium formula is with embodiment 1.

[0047] Bacillus subtilis (Bacillus subtilis), CGMCC 1.836, CGMCC 1.933, ATCC10783, ATCC 13472, and ATCC 13953 were activated on a slant, inoculated in liquid medium, and cultured at 37°C for 44 hours. Liquid culture medium formula is with embodiment 1.

[0048] Alcaligenes faecalis (Alcaligenes faecalis) CGMCC 1.1799 and CGMCC 1.2006 were activated on a slope, inoculated in a liquid medium, and cultured at 27°C for 52 hours. Liquid culture medium formula is with embodiment 1.

[0049] After activation of Penicillium diversum (Penicillium diversum) ATCC 52210 and ATCC 66706 on the slant, they were inoculated in liquid medium and cultured at 25°C for 96 hours. Liquid culture med...

Embodiment 3

[0053] (1) Carry out amplified culture respectively:

[0054] Bacillus circulans (Bacillus circulans) ATCC 13403 was activated on a slope, inoculated in a liquid medium, and cultured at 36° C. for 50 hours. Liquid culture medium formula is with embodiment 1.

[0055] Bacillus subtilis (Bacillus subtilis) CGMCC 1.933 and activated slant were inoculated in liquid medium and cultured at 30°C for 50 hours. Liquid culture medium formula is with embodiment 1.

[0056] Alcaligenes faecalis (Alcaligenes faecalis) CGMCC 1.2006 was activated on a slope, inoculated in a liquid medium, and cultured at 31° C. for 44 hours. Liquid culture medium formula is with embodiment 1.

[0057] Penicillium diversum (Penicillium diversum) ATCC 66706 was activated on a slant, inoculated in a liquid medium, and cultured at 29° C. for 72 hours. Liquid culture medium formula is with embodiment 1.

[0058] Viable bacteria count, the number of detected bacteria, mixed according to the percentage of the ...

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Abstract

The invention provides a composite microbial biogen capable of degrading organic waste efficiently and a preparation method and applications thereof. The composite microbial biogen contains Bacillus circulans, Bacillus subtilis, Alcaligenes faecalis and Penicillium diversum. The composite microbial biogen can be used with activated sludge for the existing sewage treatment equipment; under the synergetic effect, the yield of excess sludge can be greatly reduced, the aims of emission reduction and energy saving can be realized and the sludge problem which is difficult to overcome for a long time in sludge treatment is effectively solved; and the biogen has importanct economic importance and environmental importance and is suitable for each sewage treatment plant. The composite microbial biogen can also be used to prepare organic wastewater purifying agents, activated sludge enhancers of urban domestic wastewater and deodorants used in tipping sites, public places, agricultural product market, kitchens, bathrooms and the like.

Description

technical field [0001] The invention belongs to the field of microbial environmental protection, and in particular relates to a compound live microbial preparation for efficiently degrading organic waste, a preparation method and application thereof. Background technique [0002] With the continuous popularization of people's awareness of water resource protection, various places have vigorously built and expanded sewage treatment plants, and the urban sewage treatment rate has increased year by year. Although the traditional sewage treatment process can generally meet the COD standard, the ammonia nitrogen treatment effect is poor, and there is residual sludge. However, the commonly used sludge treatment methods are mainly landfill, incineration and agricultural use, but improper treatment will cause secondary pollution to the environment. Random landfilling of wet sludge causes sludge to surround the city, and harmful substances in sludge erode and leak into groundwater wi...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/20C12N1/14C02F3/34A61L9/01C12R1/09C12R1/125C12R1/05C12R1/80A61L101/52
Inventor 朱宝泉胡海峰陈纪文张琴陈建昌赵晓鸣
Owner WUXI ZHONGJIA TECH
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