Novel coronavirus recombinant antigen coating solution and pretreatment method and application thereof and product

Abstract

The invention relates to the technical field of biology, and particularly provides a novel coronavirus recombinant antigen coating solution, a pretreatment method, application and a product. The coronavirus recombinant antigen coating solution is composed of a PBS buffer solution, acidic amino acid, BSA, mannitol, trehalose, Proclin300 and water, the stability of the novel coronavirus recombinantantigen in the detection reagent can be effectively improved through mutual coordination and cooperation of all the components, and the sensitivity and specificity of the novel coronavirus antibody detection reagent are effectively improved. Besides, the inventor treats the novel coronavirus recombinant antigen by utilizing DNA hydrolase, randomly decomposes a host DNA fragment in the novel coronavirus recombinant antigen to generate oligonucleotide, eliminates the interference of the DNA fragment on a later antigen detection antibody, and has a remarkable result.

Description

technical field [0001] The present invention relates to the field of biotechnology, in particular, to a coating solution, pretreatment method, application and product of a novel coronavirus recombinant antigen. Background technique [0002] Beginning in December 2019, a novel coronavirus pneumonia (COVID-19), an acute respiratory infectious disease caused by a new type of coronavirus (SARS-CoV-2) infection, emerged. [0003] Coronaviruses are non-segmented single-stranded positive-sense RNA viruses belonging to the Orthocoronavirinae subfamily of the Coronaviridae family of the order Nidovirales. There are 6 coronaviruses known to infect humans, including 229E and NL63 of the genus α, OC43 and HKU1 of the genus β, Middle East respiratory syndrome-associated coronavirus (MERSr-CoV) and severe acute respiratory syndrome-associated coronavirus (SARSr-CoV). CoV). The coronavirus that emerged this time is a new type of coronavirus belonging to the genus Beta. Coronaviruses are...

AI Technical Summary

Problems solved by technology

[0008] Existing detection reagents for the detection of novel coronavirus antibodies directly use recombinant antigens for coating. In order to improve the sensitivity and accuracy of detection, the curre

Images