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Method for preparing rare ginsenoside by hydrolyzing ginsenoside with acidic amino acid

A technology of ginseng rare saponins and acidic amino acids, which is applied in the fields of organic chemistry and steroids, can solve the problems of strong corrosion, poor hydrolysis specificity and low yield of ginsenosides, and achieve low-cost enrichment and overcome hydrolysis specificity. one-off effect

Active Publication Date: 2016-01-27
JILIN YATAI PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The invention discloses a method for preparing rare ginsenosides by using acidic amino acids to catalyze the hydrolysis of ginsenosides, which can efficiently and environmentally friendly hydrolyze ginsenosides to prepare rare ginsenosides, and solve the problem of poor hydrolysis specificity of ginsenosides caused by the use of a large amount of strong acid and strong alkali in the existing preparation process , low yield, strong corrosiveness, environmental pollution and many by-products, etc.

Method used

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  • Method for preparing rare ginsenoside by hydrolyzing ginsenoside with acidic amino acid
  • Method for preparing rare ginsenoside by hydrolyzing ginsenoside with acidic amino acid
  • Method for preparing rare ginsenoside by hydrolyzing ginsenoside with acidic amino acid

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Effect test

Embodiment 1

[0020] Panaxadiol group saponins or triol group saponins are dissolved in water according to the mass volume ratio of 1:1 (kg / liter), and acidic amino acids are added, so that the mass ratio of panaxadiol group saponins or triol group saponins to acidic amino acids is 1:10 (kg / kg); then put it in a pressure cooker and cook at 80°C for 1 hour, take out the cooked hydrolyzate and put it on the macroporous resin column D 101, first washed with water for five column volumes, and then eluted with 60% ethanol. After recovering ethanol, the hydrolyzed product of ginseng diol group saponins obtained rare ginsenoside 20R-Rg 3 、20S-Rg 3 , Rg 5 and Rk1( figure 1 and image 3 ); Panaxatriol group saponin hydrolyzate obtains rare ginsenoside Rg 6 , F4, Rk3 and Rh4 ( figure 2 and Figure 4 ).

Embodiment 2

[0022] Panaxadiol group saponins or triol group saponins are dissolved in water according to the mass volume ratio of 1:10 (kg / liter), and acidic amino acids are added, so that the mass ratio of panaxadiol group saponins or triol group saponins to acidic amino acids is 1:1 (kg / kg); then put it in a pressure cooker and cook at 100°C for 2 hours, take out the cooked hydrolyzate and put it on the macroporous resin column D 101 , first washed with water for five column volumes, and then eluted with 70% ethanol. After recovering ethanol, the hydrolyzed products of ginsenosides of ginseng diol group obtained rare ginsenoside 20R-Rg 3 、20S-Rg 3 , Rg 5 and Rk1( figure 1 and image 3 ); Panaxatriol group saponin hydrolyzate obtains rare ginsenoside Rg 6 , F4, Rk3 and Rh4 ( figure 2 and Figure 4 ).

Embodiment 3

[0024] Panaxadiol group saponins or triol group saponins are dissolved in water according to the mass volume ratio of 1:20 (kg / liter), and acidic amino acids are added, so that the mass ratio of panaxadiol group saponins or triol group saponins to acidic amino acids is 20:1 (kg / kg); then put it in a pressure cooker and cook at 120°C for 4 hours, take out the cooked hydrolyzate and put it on the macroporous resin column D 101 , first washed with water for five column volumes, and then eluted with 80% ethanol. After recovering ethanol, the hydrolyzed products of ginsenosides of ginseng diol group obtained rare ginsenoside 20R-Rg 3 、20S-Rg 3 , Rg 5 and Rk1( figure 1 and image 3 ); Panaxatriol group saponin hydrolyzate obtains rare ginsenoside Rg 6 , F4, Rk3 and Rh4 ( figure 2 and Figure 4 ).

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Abstract

The invention discloses a method for preparing rare ginsenoside by hydrolyzing ginsenoside with acidic amino acid. Panoxadiol saponins are converted to generate 20R-Rg3, 20S-Rg3, Rg5 and Rk1, and panaxatriol saponins are converted to generate Rg6, F4, Rk3 and Rh4. Compared with an existing preparation process, the hydrolysis capacity of acidic amino acid is more than 10 times of that of neutral amino acid and that of basic amino acid and can better meet requirements of industrial preparation of rare ginsenoside, the defects that the hydrolysis specifity of ginsenoside is poor, the yield is low, the corrosion is high, environment pollution is caused, multiple byproducts are produced and the like due to the fact that a large amount of strong acid and strong base are used are overcome, ginseng resources are fully used, rare ginsenoside is extracted and enriched to the largest extent, the purpose of simple, quick, environment-friendly and low-cost enrichment of rare ginsenoside is achieved, and the method guarantees industrial production and preparation of new medicines.

Description

technical field [0001] The invention discloses a method for preparing rare ginsenosides by hydrolyzing ginsenosides with acidic amino acids, and relates to ginsenoside 20R-Rg 3 、20S-Rg 3 , Rk1, Rg 5 , Rg 6 , F4, Rk3 and Rh4 extraction, belonging to the technical field of extraction and separation of effective components of traditional Chinese medicine. Background technique [0002] In the technical field of ginseng preparation, ginsenosides are divided into diol type ginsenosides (PPD) and triol type ginsenosides (PPT) according to the structure of aglycones. Both PPD and PPT ginsenosides belong to dammarane tetracyclic triterpenoids, which account for the majority of ginsenosides. Rare ginsenosides, such as ginsenoside Rg 3 , Rg 5 , F 4 , RK1, Rh 2 、Rh 4 etc., exist or not exist in trace amounts in ginseng. The latest pharmacological studies have shown that rare ginsenosides have a variety of physiological activities, such as anti-tumor, immune regulation, anti-ce...

Claims

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Application Information

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IPC IPC(8): C07J17/00
Inventor 刘志阮长春孙光芝张金秋夏娟
Owner JILIN YATAI PHARM CO LTD
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