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Method for producing glycyrrhetinic acid monoglucuronide by fermentation, and application

A technology of glycyrrhizic acid and glycyrrhizic acid, applied in the field of bioengineering, can solve the problems of inability to meet industrial production requirements, many by-products, low yield and the like, and achieve the effects of low cost, short production cycle and high product purity

Active Publication Date: 2017-05-10
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The microbial fermentation method has the defects of low yield of the target product and many by-products, which makes the fermentation unable to meet the domestic industrial production needs

Method used

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  • Method for producing glycyrrhetinic acid monoglucuronide by fermentation, and application
  • Method for producing glycyrrhetinic acid monoglucuronide by fermentation, and application

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Embodiment 1

[0019] Each L of seed culture medium contains: peptone 5g, glucose 10g, potassium dihydrogen phosphate 1g, magnesium sulfate 0.4g.

[0020] Each L of fermentation medium contains: glycyrrhizic acid 5g, sodium nitrate 3g, potassium dihydrogen phosphate 2g, magnesium sulfate 1g, ferrous sulfate 0.01g.

[0021] The basket-shaped fungus (Talaromyces sp) was inoculated into the seed medium, and cultured at 25-40° C. for 36-48 hours. Transfer to the fermentation medium with sodium nitrate as the nitrogen source according to the volume, control the initial pH 4.5-6.5, ferment at 25-37°C for 120-144h, control the final concentration of 5g / L-glycyrrhizic acid as a carbon source and inducer.

[0022] Detect the output, purity and conversion rate of the target product in the fermentation broth after the fermentation is finished, and the results are as follows: figure 1 Shown, retention time is that the product GAMG output of 16.91 reaches 4.05g / L, and productive rate is 88.00%, and th...

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Abstract

The invention discloses a method for producing glycyrrhetinic acid monoglucuronide by fermentation, and application, and belongs to the technical field of bioengineering. According to the invention, glycyrrhizinic acid is taken as a carbon source, a talaromyces fungus is introduced to produce beta-glucuronidase, and glycyrrhetinic acid monoglucuronide can be prepared in large scale on the premise of being friendly to the environment. The method is simple in process and low in cost, the GAMG yield reaches 4.05g / L, the productivity is 88.00%, the purity is 80% or higher than 80% after purification by macroporous resin, the product purity is high, no structural alienation occurs, and the method has the advantages of being short in production cycle, easy to operate, low in energy consumption and the like, thereby being suitable for characteristics of later industrial production.

Description

technical field [0001] The invention relates to a method and application for fermenting and producing monoglucuronic acid glycyrrhetinic acid, belonging to the technical field of bioengineering. Background technique [0002] Glycyrrhiza licorice is a leguminous plant of the genus Glycyrrhizae. It is a traditional Chinese herbal medicine, and its medicinal parts are dried roots and rhizomes. Glycyrrhizin (GL), a triterpenoid compound, is the main medicinal ingredient in licorice, and it is the focus of current research on licorice. Scholars at home and abroad have conducted in-depth research on it. Glycyrrhizin (GL) has certain anti-inflammatory, hepatoprotective, Virus, anti-allergic, lipid-lowering, anti-atherosclerosis, anti-tumor, detoxification and other aspects. [0003] Glycyrrhetinic acid monoglucuronide (GAMG) is composed of one molecule of glucuronic acid and one molecule of glycyrrhetinic acid linked by glycosidic bonds. Its chemical formula is C 36 h 54 o 10 ...

Claims

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Application Information

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IPC IPC(8): C12P33/20C12R1/645
CPCC12P33/20
Inventor 赵伟郭立纯杨瑞金
Owner JIANGNAN UNIV
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